Experiment: R2A with Strontium chloride 750 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0193 and Atu0194 overlap by 26 nucleotides Atu0194 and Atu0195 are separated by 99 nucleotides Atu0195 and Atu0196 are separated by 105 nucleotides
Atu0193: Atu0193 - glutamine synthetase, at 196,923 to 198,281
Atu0193
Atu0194: Atu0194 - oxidoreductase, at 198,256 to 199,584
Atu0194
Atu0195: Atu0195 - gluconokinase, at 199,684 to 200,175
Atu0195
Atu0196: Atu0196 - ABC transporter, membrane spanning protein (proline/glycine/betaine), at 200,281 to 201,021
Atu0196
Position (kb) 198
199
200 Strain fitness (log2 ratio) -2
-1
0
1
2 at 197.446 kb on - strand, within Atu0193 at 197.510 kb on + strand, within Atu0193 at 197.518 kb on - strand, within Atu0193 at 197.678 kb on + strand, within Atu0193 at 197.883 kb on + strand, within Atu0193 at 197.888 kb on + strand, within Atu0193 at 197.888 kb on + strand, within Atu0193 at 198.194 kb on + strand at 198.261 kb on + strand at 198.275 kb on + strand at 198.322 kb on + strand at 198.368 kb on + strand at 198.434 kb on + strand, within Atu0194 at 198.455 kb on + strand, within Atu0194 at 198.463 kb on - strand, within Atu0194 at 198.487 kb on - strand, within Atu0194 at 198.499 kb on - strand, within Atu0194 at 198.648 kb on - strand, within Atu0194 at 198.868 kb on - strand, within Atu0194 at 198.868 kb on - strand, within Atu0194 at 198.868 kb on - strand, within Atu0194 at 198.878 kb on - strand, within Atu0194 at 198.967 kb on + strand, within Atu0194 at 198.975 kb on - strand, within Atu0194 at 198.975 kb on - strand, within Atu0194 at 198.985 kb on - strand, within Atu0194 at 199.094 kb on + strand, within Atu0194 at 199.108 kb on - strand, within Atu0194 at 199.204 kb on + strand, within Atu0194 at 199.204 kb on + strand, within Atu0194 at 199.212 kb on - strand, within Atu0194 at 199.213 kb on - strand, within Atu0194 at 199.213 kb on - strand, within Atu0194 at 199.431 kb on + strand, within Atu0194 at 199.439 kb on - strand, within Atu0194 at 199.456 kb on - strand at 199.473 kb on - strand at 199.617 kb on - strand at 199.762 kb on - strand, within Atu0195 at 199.813 kb on - strand, within Atu0195 at 199.813 kb on - strand, within Atu0195 at 199.855 kb on - strand, within Atu0195 at 199.855 kb on - strand, within Atu0195 at 199.855 kb on - strand, within Atu0195 at 199.974 kb on + strand, within Atu0195 at 199.978 kb on - strand, within Atu0195 at 199.995 kb on + strand, within Atu0195 at 200.010 kb on + strand, within Atu0195 at 200.134 kb on + strand at 200.141 kb on + strand at 200.149 kb on - strand at 200.199 kb on + strand at 200.207 kb on - strand at 200.395 kb on + strand, within Atu0196 at 200.481 kb on + strand, within Atu0196 at 200.544 kb on + strand, within Atu0196
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Strontium chloride 750 mM remove 197,446 - Atu0193 0.38 +0.1 197,510 + Atu0193 0.43 +0.5 197,518 - Atu0193 0.44 -0.9 197,678 + Atu0193 0.56 -0.6 197,883 + Atu0193 0.71 -0.3 197,888 + Atu0193 0.71 -0.2 197,888 + Atu0193 0.71 -0.0 198,194 + -0.1 198,261 + -1.1 198,275 + -0.3 198,322 + +0.4 198,368 + -0.1 198,434 + Atu0194 0.13 +0.7 198,455 + Atu0194 0.15 -0.6 198,463 - Atu0194 0.16 -2.7 198,487 - Atu0194 0.17 -0.0 198,499 - Atu0194 0.18 -0.2 198,648 - Atu0194 0.29 +0.4 198,868 - Atu0194 0.46 +0.5 198,868 - Atu0194 0.46 +0.4 198,868 - Atu0194 0.46 -0.2 198,878 - Atu0194 0.47 -0.3 198,967 + Atu0194 0.53 -0.1 198,975 - Atu0194 0.54 +0.6 198,975 - Atu0194 0.54 -1.1 198,985 - Atu0194 0.55 -1.3 199,094 + Atu0194 0.63 -0.1 199,108 - Atu0194 0.64 +0.4 199,204 + Atu0194 0.71 -0.2 199,204 + Atu0194 0.71 +1.1 199,212 - Atu0194 0.72 -0.2 199,213 - Atu0194 0.72 -1.5 199,213 - Atu0194 0.72 -0.0 199,431 + Atu0194 0.88 +1.3 199,439 - Atu0194 0.89 -0.1 199,456 - +0.2 199,473 - +2.5 199,617 - +0.9 199,762 - Atu0195 0.16 +0.3 199,813 - Atu0195 0.26 +1.3 199,813 - Atu0195 0.26 +0.7 199,855 - Atu0195 0.35 +1.1 199,855 - Atu0195 0.35 -0.0 199,855 - Atu0195 0.35 -0.3 199,974 + Atu0195 0.59 +0.1 199,978 - Atu0195 0.60 +0.2 199,995 + Atu0195 0.63 -0.0 200,010 + Atu0195 0.66 -0.0 200,134 + -1.0 200,141 + -0.1 200,149 - -0.1 200,199 + -0.2 200,207 - +0.1 200,395 + Atu0196 0.15 +1.2 200,481 + Atu0196 0.27 -0.0 200,544 + Atu0196 0.35 +0.3
Or see this region's nucleotide sequence