Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF847 and GFF848 are separated by 15 nucleotides GFF848 and GFF849 are separated by 9 nucleotides GFF849 and GFF850 are separated by 13 nucleotides
GFF847 - Ascorbate-specific PTS system, EIIC component, at 28,582 to 29,979
GFF847
GFF848 - Ascorbate-specific PTS system, EIIB component (EC 2.7.1.69), at 29,995 to 30,300
GFF848
GFF849 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 30,310 to 30,774
GFF849
GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438
GFF850
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 29.026 kb on + strand, within GFF847 at 29.026 kb on + strand, within GFF847 at 29.058 kb on + strand, within GFF847 at 29.098 kb on - strand, within GFF847 at 29.113 kb on + strand, within GFF847 at 29.113 kb on + strand, within GFF847 at 29.114 kb on - strand, within GFF847 at 29.114 kb on - strand, within GFF847 at 29.191 kb on + strand, within GFF847 at 29.192 kb on - strand, within GFF847 at 29.194 kb on - strand, within GFF847 at 29.297 kb on + strand, within GFF847 at 29.308 kb on + strand, within GFF847 at 29.386 kb on - strand, within GFF847 at 29.445 kb on + strand, within GFF847 at 29.446 kb on - strand, within GFF847 at 29.446 kb on - strand, within GFF847 at 29.446 kb on - strand, within GFF847 at 29.533 kb on - strand, within GFF847 at 29.572 kb on - strand, within GFF847 at 29.641 kb on - strand, within GFF847 at 29.641 kb on - strand, within GFF847 at 29.907 kb on + strand at 29.908 kb on - strand at 29.908 kb on - strand at 29.908 kb on - strand at 30.002 kb on + strand at 30.002 kb on + strand at 30.006 kb on + strand at 30.006 kb on + strand at 30.007 kb on - strand at 30.007 kb on - strand at 30.172 kb on - strand, within GFF848 at 30.205 kb on + strand, within GFF848 at 30.298 kb on + strand at 30.369 kb on + strand, within GFF849 at 30.772 kb on + strand at 31.009 kb on + strand, within GFF850 at 31.010 kb on - strand, within GFF850 at 31.191 kb on - strand, within GFF850
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 29,026 + GFF847 0.32 +0.1 29,026 + GFF847 0.32 -0.7 29,058 + GFF847 0.34 -0.1 29,098 - GFF847 0.37 +0.6 29,113 + GFF847 0.38 -0.1 29,113 + GFF847 0.38 +0.9 29,114 - GFF847 0.38 -0.5 29,114 - GFF847 0.38 +0.8 29,191 + GFF847 0.44 -0.1 29,192 - GFF847 0.44 +0.3 29,194 - GFF847 0.44 +1.0 29,297 + GFF847 0.51 +1.3 29,308 + GFF847 0.52 -0.8 29,386 - GFF847 0.58 -0.3 29,445 + GFF847 0.62 +0.1 29,446 - GFF847 0.62 +0.5 29,446 - GFF847 0.62 -0.2 29,446 - GFF847 0.62 -0.4 29,533 - GFF847 0.68 +0.4 29,572 - GFF847 0.71 +0.3 29,641 - GFF847 0.76 -3.2 29,641 - GFF847 0.76 -0.4 29,907 + +0.4 29,908 - +0.3 29,908 - -0.4 29,908 - -0.3 30,002 + +0.2 30,002 + +0.8 30,006 + +0.4 30,006 + -0.8 30,007 - +0.2 30,007 - +0.8 30,172 - GFF848 0.58 +0.3 30,205 + GFF848 0.69 -0.5 30,298 + -0.1 30,369 + GFF849 0.13 -0.0 30,772 + -0.3 31,009 + GFF850 0.34 +0.2 31,010 - GFF850 0.34 -0.2 31,191 - GFF850 0.62 +0.2
Or see this region's nucleotide sequence