Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF684 and GFF685 are separated by 32 nucleotides GFF685 and GFF686 are separated by 7 nucleotides GFF686 and GFF687 are separated by 2 nucleotides GFF687 and GFF688 overlap by 1 nucleotides
GFF684 - Putative membrane protein, at 41,490 to 42,011
GFF684
GFF685 - Putative inner membrane protein, at 42,044 to 42,643
GFF685
GFF686 - Putative inner membrane protein, at 42,651 to 43,211
GFF686
GFF687 - Inner membrane protein YbhQ, at 43,214 to 44,176
GFF687
GFF688 - Cardiolipin synthetase (EC 2.7.8.-), at 44,176 to 45,417
GFF688
Position (kb) 42
43
44 Strain fitness (log2 ratio) -1
0
1 at 41.804 kb on - strand, within GFF684 at 41.830 kb on + strand, within GFF684 at 42.595 kb on - strand at 42.782 kb on + strand, within GFF686 at 42.858 kb on - strand, within GFF686 at 42.881 kb on + strand, within GFF686 at 43.094 kb on + strand, within GFF686 at 43.095 kb on - strand, within GFF686 at 43.153 kb on - strand, within GFF686 at 43.153 kb on - strand, within GFF686 at 43.153 kb on - strand, within GFF686 at 43.363 kb on + strand, within GFF687 at 43.363 kb on + strand, within GFF687 at 43.609 kb on + strand, within GFF687 at 43.610 kb on - strand, within GFF687 at 43.644 kb on + strand, within GFF687 at 43.645 kb on - strand, within GFF687 at 43.645 kb on - strand, within GFF687 at 43.654 kb on + strand, within GFF687 at 43.738 kb on + strand, within GFF687 at 43.763 kb on - strand, within GFF687 at 43.783 kb on + strand, within GFF687 at 43.810 kb on + strand, within GFF687 at 43.813 kb on + strand, within GFF687 at 43.814 kb on - strand, within GFF687 at 43.839 kb on + strand, within GFF687 at 43.900 kb on - strand, within GFF687 at 43.996 kb on + strand, within GFF687 at 44.022 kb on - strand, within GFF687 at 44.171 kb on - strand at 44.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 41,804 - GFF684 0.60 +0.4 41,830 + GFF684 0.65 +0.1 42,595 - -0.0 42,782 + GFF686 0.23 -0.4 42,858 - GFF686 0.37 +0.0 42,881 + GFF686 0.41 -1.0 43,094 + GFF686 0.79 -0.2 43,095 - GFF686 0.79 -0.1 43,153 - GFF686 0.89 +0.3 43,153 - GFF686 0.89 -0.4 43,153 - GFF686 0.89 -0.0 43,363 + GFF687 0.15 -0.3 43,363 + GFF687 0.15 -0.0 43,609 + GFF687 0.41 +0.9 43,610 - GFF687 0.41 +0.1 43,644 + GFF687 0.45 +0.5 43,645 - GFF687 0.45 -0.2 43,645 - GFF687 0.45 +0.3 43,654 + GFF687 0.46 -0.4 43,738 + GFF687 0.54 +0.0 43,763 - GFF687 0.57 -0.4 43,783 + GFF687 0.59 -0.7 43,810 + GFF687 0.62 -0.1 43,813 + GFF687 0.62 -0.6 43,814 - GFF687 0.62 -0.1 43,839 + GFF687 0.65 +0.1 43,900 - GFF687 0.71 -0.7 43,996 + GFF687 0.81 -0.5 44,022 - GFF687 0.84 +0.2 44,171 - +0.1 44,171 - +0.6
Or see this region's nucleotide sequence