Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF544 and GFF545 are separated by 321 nucleotides GFF545 and GFF546 are separated by 82 nucleotides GFF546 and GFF547 overlap by 23 nucleotides
GFF544 - RNA polymerase sigma factor RpoH, at 276,297 to 277,151
GFF544
GFF545 - High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1), at 277,473 to 278,576
GFF545
GFF546 - hypothetical protein, at 278,659 to 278,793
GFF546
GFF547 - probable acetyltransferase YPO3809, at 278,771 to 279,154
GFF547
Position (kb) 277
278
279 Strain fitness (log2 ratio) -1
0
1
2 at 277.244 kb on - strand at 277.325 kb on - strand at 277.427 kb on + strand at 277.478 kb on + strand at 277.479 kb on - strand at 277.655 kb on + strand, within GFF545 at 277.668 kb on - strand, within GFF545 at 277.686 kb on + strand, within GFF545 at 277.686 kb on + strand, within GFF545 at 277.755 kb on + strand, within GFF545 at 277.755 kb on + strand, within GFF545 at 277.771 kb on + strand, within GFF545 at 277.772 kb on - strand, within GFF545 at 277.772 kb on - strand, within GFF545 at 277.772 kb on - strand, within GFF545 at 277.803 kb on - strand, within GFF545 at 277.806 kb on + strand, within GFF545 at 277.806 kb on + strand, within GFF545 at 277.807 kb on - strand, within GFF545 at 277.807 kb on - strand, within GFF545 at 277.807 kb on - strand, within GFF545 at 277.807 kb on - strand, within GFF545 at 277.807 kb on - strand, within GFF545 at 277.831 kb on + strand, within GFF545 at 277.932 kb on + strand, within GFF545 at 277.932 kb on + strand, within GFF545 at 277.933 kb on - strand, within GFF545 at 278.137 kb on - strand, within GFF545 at 278.272 kb on + strand, within GFF545 at 278.272 kb on + strand, within GFF545 at 278.273 kb on - strand, within GFF545 at 278.383 kb on - strand, within GFF545 at 278.448 kb on + strand, within GFF545 at 278.612 kb on + strand at 278.635 kb on + strand at 278.635 kb on + strand at 278.635 kb on + strand at 278.635 kb on + strand at 278.639 kb on + strand at 278.640 kb on - strand at 278.640 kb on - strand at 278.640 kb on - strand at 278.643 kb on + strand at 278.643 kb on + strand at 278.644 kb on - strand at 278.644 kb on - strand at 278.803 kb on + strand at 278.909 kb on + strand, within GFF547 at 278.909 kb on + strand, within GFF547 at 278.917 kb on - strand, within GFF547 at 279.000 kb on - strand, within GFF547 at 279.057 kb on - strand, within GFF547 at 279.131 kb on + strand at 279.283 kb on + strand at 279.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 277,244 - +0.8 277,325 - +0.5 277,427 + -0.6 277,478 + -0.3 277,479 - -0.3 277,655 + GFF545 0.16 -0.9 277,668 - GFF545 0.18 -0.4 277,686 + GFF545 0.19 +0.0 277,686 + GFF545 0.19 +0.4 277,755 + GFF545 0.26 -0.4 277,755 + GFF545 0.26 -0.1 277,771 + GFF545 0.27 -0.1 277,772 - GFF545 0.27 +0.8 277,772 - GFF545 0.27 +0.8 277,772 - GFF545 0.27 +0.3 277,803 - GFF545 0.30 +2.5 277,806 + GFF545 0.30 -0.5 277,806 + GFF545 0.30 +0.0 277,807 - GFF545 0.30 +0.3 277,807 - GFF545 0.30 -0.1 277,807 - GFF545 0.30 -0.3 277,807 - GFF545 0.30 +0.1 277,807 - GFF545 0.30 -0.4 277,831 + GFF545 0.32 +0.3 277,932 + GFF545 0.42 -0.1 277,932 + GFF545 0.42 -0.6 277,933 - GFF545 0.42 +0.1 278,137 - GFF545 0.60 +0.1 278,272 + GFF545 0.72 -0.2 278,272 + GFF545 0.72 +0.7 278,273 - GFF545 0.72 -0.1 278,383 - GFF545 0.82 -0.1 278,448 + GFF545 0.88 +0.2 278,612 + -0.9 278,635 + +0.0 278,635 + +0.4 278,635 + +0.1 278,635 + +0.3 278,639 + +0.3 278,640 - -0.6 278,640 - -0.5 278,640 - -0.2 278,643 + +0.3 278,643 + +0.3 278,644 - -0.5 278,644 - +0.5 278,803 + -0.3 278,909 + GFF547 0.36 +0.4 278,909 + GFF547 0.36 -1.3 278,917 - GFF547 0.38 -0.1 279,000 - GFF547 0.60 -1.1 279,057 - GFF547 0.74 +0.2 279,131 + -0.1 279,283 + +1.4 279,547 + -0.2
Or see this region's nucleotide sequence