Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF497 and GFF498 are separated by 53 nucleotides GFF498 and GFF499 are separated by 58 nucleotides GFF499 and GFF500 are separated by 109 nucleotides
GFF497 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 224,569 to 225,498
GFF497
GFF498 - hypothetical protein, at 225,552 to 225,668
GFF498
GFF499 - GGDEF/EAL domain protein YhjH, at 225,727 to 226,494
GFF499
GFF500 - Uncharacterized protein YhjG, at 226,604 to 228,664
GFF500
Position (kb) 225
226 Strain fitness (log2 ratio) -1
0
1 at 224.728 kb on + strand, within GFF497 at 224.818 kb on + strand, within GFF497 at 224.949 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 225.090 kb on + strand, within GFF497 at 225.110 kb on + strand, within GFF497 at 225.110 kb on + strand, within GFF497 at 225.197 kb on + strand, within GFF497 at 225.234 kb on - strand, within GFF497 at 225.234 kb on - strand, within GFF497 at 225.236 kb on + strand, within GFF497 at 225.236 kb on + strand, within GFF497 at 225.237 kb on - strand, within GFF497 at 225.280 kb on - strand, within GFF497 at 225.350 kb on + strand, within GFF497 at 225.351 kb on - strand, within GFF497 at 225.390 kb on + strand, within GFF497 at 225.390 kb on + strand, within GFF497 at 225.392 kb on + strand, within GFF497 at 225.392 kb on + strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.498 kb on + strand at 225.499 kb on - strand at 225.732 kb on - strand at 225.732 kb on - strand at 225.752 kb on + strand at 225.771 kb on + strand at 225.820 kb on + strand, within GFF499 at 225.826 kb on + strand, within GFF499 at 225.839 kb on - strand, within GFF499 at 225.839 kb on - strand, within GFF499 at 225.912 kb on + strand, within GFF499 at 226.025 kb on + strand, within GFF499 at 226.025 kb on + strand, within GFF499 at 226.107 kb on - strand, within GFF499 at 226.137 kb on + strand, within GFF499 at 226.137 kb on + strand, within GFF499 at 226.261 kb on - strand, within GFF499 at 226.521 kb on + strand at 226.524 kb on + strand at 226.525 kb on - strand at 226.525 kb on - strand at 226.610 kb on - strand at 226.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 224,728 + GFF497 0.17 +0.3 224,818 + GFF497 0.27 -0.1 224,949 - GFF497 0.41 +0.4 224,998 - GFF497 0.46 -0.3 224,998 - GFF497 0.46 -0.4 224,998 - GFF497 0.46 +0.2 225,090 + GFF497 0.56 +0.4 225,110 + GFF497 0.58 +0.4 225,110 + GFF497 0.58 -0.2 225,197 + GFF497 0.68 -0.2 225,234 - GFF497 0.72 -0.1 225,234 - GFF497 0.72 +1.4 225,236 + GFF497 0.72 +0.1 225,236 + GFF497 0.72 +0.0 225,237 - GFF497 0.72 -0.6 225,280 - GFF497 0.76 +0.9 225,350 + GFF497 0.84 +0.1 225,351 - GFF497 0.84 +0.2 225,390 + GFF497 0.88 -0.2 225,390 + GFF497 0.88 +0.0 225,392 + GFF497 0.88 -0.4 225,392 + GFF497 0.88 -0.3 225,403 - GFF497 0.90 +0.2 225,403 - GFF497 0.90 -0.6 225,403 - GFF497 0.90 +0.0 225,498 + +0.2 225,499 - -0.2 225,732 - +0.4 225,732 - +0.7 225,752 + -0.1 225,771 + +0.1 225,820 + GFF499 0.12 +0.6 225,826 + GFF499 0.13 +0.0 225,839 - GFF499 0.15 +0.2 225,839 - GFF499 0.15 +0.2 225,912 + GFF499 0.24 +1.5 226,025 + GFF499 0.39 +1.0 226,025 + GFF499 0.39 -0.3 226,107 - GFF499 0.49 -0.2 226,137 + GFF499 0.53 +0.4 226,137 + GFF499 0.53 -0.0 226,261 - GFF499 0.70 -0.9 226,521 + +0.1 226,524 + +0.3 226,525 - -0.4 226,525 - -0.3 226,610 - -0.0 226,665 + -0.1
Or see this region's nucleotide sequence