Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4584 and GFF4585 are separated by 4 nucleotides GFF4585 and GFF4586 are separated by 59 nucleotides
GFF4584 - N-acetylglucosamine-6P-responsive transcriptional repressor NagC, ROK family, at 372,236 to 373,456
GFF4584
GFF4585 - N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25), at 373,461 to 374,615
GFF4585
GFF4586 - Glucosamine-6-phosphate deaminase (EC 3.5.99.6), at 374,675 to 375,475
GFF4586
Position (kb) 373
374
375 Strain fitness (log2 ratio) -2
-1
0
1 at 372.553 kb on + strand, within GFF4584 at 372.611 kb on - strand, within GFF4584 at 372.844 kb on - strand, within GFF4584 at 373.042 kb on + strand, within GFF4584 at 373.043 kb on - strand, within GFF4584 at 373.135 kb on + strand, within GFF4584 at 373.135 kb on + strand, within GFF4584 at 373.150 kb on - strand, within GFF4584 at 373.158 kb on - strand, within GFF4584 at 373.229 kb on - strand, within GFF4584 at 373.427 kb on - strand at 373.457 kb on - strand at 373.466 kb on + strand at 373.704 kb on - strand, within GFF4585 at 373.892 kb on - strand, within GFF4585 at 373.892 kb on - strand, within GFF4585 at 373.948 kb on + strand, within GFF4585 at 374.319 kb on - strand, within GFF4585 at 374.337 kb on - strand, within GFF4585 at 374.339 kb on + strand, within GFF4585 at 374.340 kb on - strand, within GFF4585 at 374.340 kb on - strand, within GFF4585 at 374.340 kb on - strand, within GFF4585 at 374.406 kb on - strand, within GFF4585 at 374.612 kb on - strand at 374.980 kb on + strand, within GFF4586 at 374.981 kb on - strand, within GFF4586 at 375.266 kb on - strand, within GFF4586 at 375.403 kb on + strand at 375.404 kb on - strand at 375.404 kb on - strand at 375.407 kb on - strand at 375.524 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 372,553 + GFF4584 0.26 +1.0 372,611 - GFF4584 0.31 -0.4 372,844 - GFF4584 0.50 +0.8 373,042 + GFF4584 0.66 -1.2 373,043 - GFF4584 0.66 -0.5 373,135 + GFF4584 0.74 -0.7 373,135 + GFF4584 0.74 +0.1 373,150 - GFF4584 0.75 -1.0 373,158 - GFF4584 0.76 -0.4 373,229 - GFF4584 0.81 -0.4 373,427 - -0.4 373,457 - -0.2 373,466 + -0.7 373,704 - GFF4585 0.21 -0.0 373,892 - GFF4585 0.37 -0.2 373,892 - GFF4585 0.37 +0.1 373,948 + GFF4585 0.42 -1.1 374,319 - GFF4585 0.74 -0.5 374,337 - GFF4585 0.76 -0.8 374,339 + GFF4585 0.76 -2.2 374,340 - GFF4585 0.76 -0.4 374,340 - GFF4585 0.76 -0.1 374,340 - GFF4585 0.76 -0.0 374,406 - GFF4585 0.82 -0.6 374,612 - -0.3 374,980 + GFF4586 0.38 -1.3 374,981 - GFF4586 0.38 -0.2 375,266 - GFF4586 0.74 -0.5 375,403 + -0.2 375,404 - -0.3 375,404 - +0.6 375,407 - -0.3 375,524 + -0.3
Or see this region's nucleotide sequence