Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4513 and GFF4514 are separated by 241 nucleotides GFF4514 and GFF4515 are separated by 331 nucleotides
GFF4513 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 300,663 to 301,226
GFF4513
GFF4514 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 301,468 to 303,033
GFF4514
GFF4515 - Putative oxidoreductase component of anaerobic dehydrogenases; Chaperone protein TorD, at 303,365 to 303,925
GFF4515
Position (kb) 301
302
303
304 Strain fitness (log2 ratio) -1
0
1 at 300.568 kb on - strand at 300.791 kb on + strand, within GFF4513 at 300.834 kb on + strand, within GFF4513 at 300.835 kb on - strand, within GFF4513 at 300.943 kb on - strand, within GFF4513 at 301.156 kb on + strand, within GFF4513 at 301.285 kb on + strand at 301.285 kb on + strand at 301.483 kb on - strand at 301.635 kb on + strand, within GFF4514 at 301.668 kb on + strand, within GFF4514 at 301.915 kb on - strand, within GFF4514 at 302.022 kb on + strand, within GFF4514 at 302.022 kb on + strand, within GFF4514 at 302.022 kb on + strand, within GFF4514 at 302.104 kb on + strand, within GFF4514 at 302.105 kb on - strand, within GFF4514 at 302.112 kb on - strand, within GFF4514 at 302.239 kb on + strand, within GFF4514 at 302.240 kb on - strand, within GFF4514 at 302.240 kb on - strand, within GFF4514 at 302.419 kb on - strand, within GFF4514 at 302.452 kb on - strand, within GFF4514 at 302.497 kb on + strand, within GFF4514 at 302.600 kb on + strand, within GFF4514 at 302.610 kb on + strand, within GFF4514 at 302.610 kb on + strand, within GFF4514 at 303.041 kb on + strand at 303.094 kb on - strand at 303.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 300,568 - -0.1 300,791 + GFF4513 0.23 -0.3 300,834 + GFF4513 0.30 -0.2 300,835 - GFF4513 0.30 +0.1 300,943 - GFF4513 0.50 -0.0 301,156 + GFF4513 0.87 +0.2 301,285 + +0.1 301,285 + +0.9 301,483 - +0.8 301,635 + GFF4514 0.11 -0.7 301,668 + GFF4514 0.13 +0.3 301,915 - GFF4514 0.29 +0.3 302,022 + GFF4514 0.35 -0.6 302,022 + GFF4514 0.35 -0.1 302,022 + GFF4514 0.35 +0.0 302,104 + GFF4514 0.41 -1.0 302,105 - GFF4514 0.41 -0.6 302,112 - GFF4514 0.41 -0.2 302,239 + GFF4514 0.49 +0.1 302,240 - GFF4514 0.49 -0.5 302,240 - GFF4514 0.49 -0.3 302,419 - GFF4514 0.61 -0.1 302,452 - GFF4514 0.63 -0.1 302,497 + GFF4514 0.66 -0.4 302,600 + GFF4514 0.72 -0.2 302,610 + GFF4514 0.73 -0.4 302,610 + GFF4514 0.73 -0.1 303,041 + +0.1 303,094 - +0.2 303,880 - +0.3
Or see this region's nucleotide sequence