Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4359 and GFF4360 are separated by 105 nucleotides GFF4360 and GFF4361 are separated by 64 nucleotides GFF4361 and GFF4362 are separated by 100 nucleotides GFF4362 and GFF4363 are separated by 49 nucleotides
GFF4359 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 141,379 to 141,777
GFF4359
GFF4360 - Queuosine Biosynthesis QueC ATPase, at 141,883 to 142,578
GFF4360
GFF4361 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 142,643 to 144,343
GFF4361
GFF4362 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 144,444 to 145,262
GFF4362
GFF4363 - Cysteine synthase B (EC 2.5.1.47), at 145,312 to 146,367
GFF4363
Position (kb) 142
143
144
145 Strain fitness (log2 ratio) -2
-1
0
1 at 141.821 kb on - strand at 141.836 kb on + strand at 141.890 kb on - strand at 142.003 kb on - strand, within GFF4360 at 142.087 kb on - strand, within GFF4360 at 142.099 kb on + strand, within GFF4360 at 142.506 kb on - strand, within GFF4360 at 142.559 kb on - strand at 142.579 kb on - strand at 142.579 kb on - strand at 142.579 kb on - strand at 142.582 kb on + strand at 142.750 kb on + strand at 142.917 kb on - strand, within GFF4361 at 142.933 kb on + strand, within GFF4361 at 143.096 kb on + strand, within GFF4361 at 143.109 kb on + strand, within GFF4361 at 143.201 kb on - strand, within GFF4361 at 143.242 kb on + strand, within GFF4361 at 143.254 kb on + strand, within GFF4361 at 143.255 kb on - strand, within GFF4361 at 143.255 kb on - strand, within GFF4361 at 143.258 kb on + strand, within GFF4361 at 143.280 kb on - strand, within GFF4361 at 143.313 kb on + strand, within GFF4361 at 143.333 kb on - strand, within GFF4361 at 143.338 kb on + strand, within GFF4361 at 143.338 kb on + strand, within GFF4361 at 143.339 kb on - strand, within GFF4361 at 143.339 kb on - strand, within GFF4361 at 143.342 kb on + strand, within GFF4361 at 143.342 kb on + strand, within GFF4361 at 143.343 kb on - strand, within GFF4361 at 143.484 kb on + strand, within GFF4361 at 143.589 kb on + strand, within GFF4361 at 143.590 kb on - strand, within GFF4361 at 143.590 kb on - strand, within GFF4361 at 143.710 kb on + strand, within GFF4361 at 143.764 kb on + strand, within GFF4361 at 143.962 kb on - strand, within GFF4361 at 143.986 kb on + strand, within GFF4361 at 144.066 kb on - strand, within GFF4361 at 144.335 kb on + strand at 144.381 kb on - strand at 144.468 kb on - strand at 144.481 kb on + strand at 144.484 kb on + strand at 144.485 kb on - strand at 144.625 kb on - strand, within GFF4362 at 145.243 kb on + strand at 145.243 kb on + strand at 145.244 kb on - strand at 145.244 kb on - strand at 145.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 141,821 - -0.2 141,836 + -0.5 141,890 - -0.3 142,003 - GFF4360 0.17 -0.2 142,087 - GFF4360 0.29 -1.0 142,099 + GFF4360 0.31 -0.1 142,506 - GFF4360 0.90 +1.3 142,559 - -0.5 142,579 - -0.5 142,579 - -0.1 142,579 - -0.4 142,582 + -1.9 142,750 + +0.3 142,917 - GFF4361 0.16 -0.0 142,933 + GFF4361 0.17 -1.0 143,096 + GFF4361 0.27 -0.1 143,109 + GFF4361 0.27 +0.6 143,201 - GFF4361 0.33 +0.6 143,242 + GFF4361 0.35 -0.5 143,254 + GFF4361 0.36 +0.7 143,255 - GFF4361 0.36 +0.2 143,255 - GFF4361 0.36 +0.1 143,258 + GFF4361 0.36 -0.2 143,280 - GFF4361 0.37 -0.8 143,313 + GFF4361 0.39 +1.4 143,333 - GFF4361 0.41 -0.1 143,338 + GFF4361 0.41 -0.2 143,338 + GFF4361 0.41 +0.3 143,339 - GFF4361 0.41 +0.5 143,339 - GFF4361 0.41 +1.4 143,342 + GFF4361 0.41 +0.2 143,342 + GFF4361 0.41 +0.9 143,343 - GFF4361 0.41 +0.2 143,484 + GFF4361 0.49 +0.1 143,589 + GFF4361 0.56 +0.1 143,590 - GFF4361 0.56 +0.2 143,590 - GFF4361 0.56 +0.2 143,710 + GFF4361 0.63 +1.0 143,764 + GFF4361 0.66 -0.6 143,962 - GFF4361 0.78 +1.2 143,986 + GFF4361 0.79 +0.7 144,066 - GFF4361 0.84 -0.9 144,335 + +0.3 144,381 - -0.1 144,468 - -0.2 144,481 + -0.4 144,484 + +0.6 144,485 - -0.5 144,625 - GFF4362 0.22 -0.1 145,243 + +0.4 145,243 + -0.2 145,244 - +0.8 145,244 - -0.2 145,249 - -1.0
Or see this region's nucleotide sequence