Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4353 and GFF4354 are separated by 251 nucleotides GFF4354 and GFF4355 are separated by 185 nucleotides
GFF4353 - ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92), at 133,314 to 134,045
GFF4353
GFF4354 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 134,297 to 135,568
GFF4354
GFF4355 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 135,754 to 138,108
GFF4355
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-2
-1
0
1 at 133.518 kb on - strand, within GFF4353 at 133.522 kb on - strand, within GFF4353 at 133.577 kb on + strand, within GFF4353 at 133.577 kb on + strand, within GFF4353 at 133.693 kb on - strand, within GFF4353 at 133.710 kb on - strand, within GFF4353 at 133.737 kb on - strand, within GFF4353 at 133.964 kb on + strand, within GFF4353 at 134.007 kb on + strand at 134.007 kb on + strand at 134.007 kb on + strand at 134.071 kb on + strand at 134.129 kb on - strand at 134.132 kb on + strand at 134.136 kb on + strand at 134.136 kb on + strand at 134.194 kb on + strand at 134.195 kb on - strand at 134.196 kb on + strand at 134.196 kb on + strand at 134.196 kb on + strand at 134.196 kb on + strand at 134.199 kb on + strand at 134.200 kb on - strand at 134.401 kb on + strand at 134.401 kb on + strand at 134.429 kb on - strand, within GFF4354 at 134.525 kb on + strand, within GFF4354 at 134.630 kb on - strand, within GFF4354 at 134.719 kb on + strand, within GFF4354 at 135.010 kb on - strand, within GFF4354 at 135.013 kb on + strand, within GFF4354 at 135.449 kb on + strand at 135.449 kb on + strand at 135.671 kb on + strand at 135.679 kb on + strand at 135.680 kb on - strand at 135.689 kb on - strand at 135.708 kb on + strand at 135.708 kb on + strand at 135.708 kb on + strand at 135.709 kb on - strand at 135.709 kb on - strand at 135.710 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 133,518 - GFF4353 0.28 -0.4 133,522 - GFF4353 0.28 -0.6 133,577 + GFF4353 0.36 +0.1 133,577 + GFF4353 0.36 -1.3 133,693 - GFF4353 0.52 +0.0 133,710 - GFF4353 0.54 -0.5 133,737 - GFF4353 0.58 -0.1 133,964 + GFF4353 0.89 -1.0 134,007 + -0.7 134,007 + +0.6 134,007 + -0.7 134,071 + -0.2 134,129 - +0.2 134,132 + -0.2 134,136 + +0.2 134,136 + -1.0 134,194 + +0.3 134,195 - -0.7 134,196 + -0.7 134,196 + -0.3 134,196 + -0.2 134,196 + -1.4 134,199 + -0.3 134,200 - -0.5 134,401 + -1.0 134,401 + -1.3 134,429 - GFF4354 0.10 -2.2 134,525 + GFF4354 0.18 -1.4 134,630 - GFF4354 0.26 -0.5 134,719 + GFF4354 0.33 -1.4 135,010 - GFF4354 0.56 -2.6 135,013 + GFF4354 0.56 -0.5 135,449 + -0.7 135,449 + -1.2 135,671 + -0.4 135,679 + -0.6 135,680 - -0.5 135,689 - -1.9 135,708 + -0.4 135,708 + -1.0 135,708 + -0.3 135,709 - -1.5 135,709 - -0.5 135,710 + -0.3
Or see this region's nucleotide sequence