Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4257 and GFF4258 are separated by 163 nucleotides GFF4258 and GFF4259 are separated by 40 nucleotides GFF4259 and GFF4260 are separated by 231 nucleotides
GFF4257 - Transcriptional regulator, AraC family, at 41,725 to 42,729
GFF4257
GFF4258 - Ferrichrome-iron receptor, at 42,893 to 44,983
GFF4258
GFF4259 - Threonine efflux protein, at 45,024 to 45,656
GFF4259
GFF4260 - FIG00639237: hypothetical protein, at 45,888 to 46,163
GFF4260
Position (kb)
42
43
44
45 Strain fitness (log2 ratio)
-2
-1
0
1 at 41.917 kb on - strand, within GFF4257 at 41.917 kb on - strand, within GFF4257 at 41.939 kb on + strand, within GFF4257 at 41.939 kb on + strand, within GFF4257 at 41.940 kb on - strand, within GFF4257 at 41.940 kb on - strand, within GFF4257 at 41.947 kb on + strand, within GFF4257 at 41.948 kb on - strand, within GFF4257 at 41.980 kb on + strand, within GFF4257 at 41.981 kb on - strand, within GFF4257 at 41.981 kb on - strand, within GFF4257 at 41.981 kb on - strand, within GFF4257 at 41.982 kb on + strand, within GFF4257 at 41.982 kb on + strand, within GFF4257 at 41.983 kb on - strand, within GFF4257 at 42.004 kb on - strand, within GFF4257 at 42.142 kb on + strand, within GFF4257 at 42.299 kb on + strand, within GFF4257 at 42.334 kb on + strand, within GFF4257 at 42.521 kb on - strand, within GFF4257 at 42.521 kb on - strand, within GFF4257 at 42.827 kb on + strand at 42.828 kb on - strand at 42.828 kb on - strand at 42.865 kb on + strand at 43.069 kb on + strand at 43.142 kb on + strand, within GFF4258 at 43.178 kb on + strand, within GFF4258 at 43.186 kb on - strand, within GFF4258 at 43.186 kb on - strand, within GFF4258 at 43.207 kb on + strand, within GFF4258 at 43.223 kb on + strand, within GFF4258 at 43.427 kb on - strand, within GFF4258 at 43.585 kb on + strand, within GFF4258 at 43.609 kb on + strand, within GFF4258 at 43.685 kb on - strand, within GFF4258 at 43.731 kb on - strand, within GFF4258 at 43.769 kb on - strand, within GFF4258 at 43.853 kb on + strand, within GFF4258 at 44.020 kb on + strand, within GFF4258 at 44.020 kb on + strand, within GFF4258 at 44.024 kb on - strand, within GFF4258 at 44.073 kb on - strand, within GFF4258 at 44.095 kb on + strand, within GFF4258 at 44.096 kb on - strand, within GFF4258 at 44.103 kb on - strand, within GFF4258 at 44.103 kb on - strand, within GFF4258 at 44.104 kb on + strand, within GFF4258 at 44.141 kb on + strand, within GFF4258 at 44.142 kb on - strand, within GFF4258 at 44.179 kb on + strand, within GFF4258 at 44.465 kb on - strand, within GFF4258 at 44.501 kb on - strand, within GFF4258 at 44.536 kb on + strand, within GFF4258 at 44.536 kb on + strand, within GFF4258 at 44.598 kb on - strand, within GFF4258 at 44.600 kb on - strand, within GFF4258 at 44.603 kb on + strand, within GFF4258 at 44.603 kb on + strand, within GFF4258 at 44.603 kb on + strand, within GFF4258 at 44.603 kb on + strand, within GFF4258 at 44.603 kb on + strand, within GFF4258 at 44.604 kb on - strand, within GFF4258 at 44.605 kb on + strand, within GFF4258 at 44.605 kb on + strand, within GFF4258 at 44.618 kb on + strand, within GFF4258 at 44.696 kb on + strand, within GFF4258 at 44.783 kb on - strand at 44.801 kb on + strand at 44.801 kb on + strand at 44.802 kb on - strand at 44.803 kb on + strand at 44.804 kb on - strand at 44.804 kb on - strand at 44.823 kb on + strand at 44.829 kb on - strand at 44.850 kb on + strand at 44.927 kb on + strand at 44.928 kb on - strand at 44.928 kb on - strand at 44.958 kb on + strand at 44.985 kb on - strand at 45.047 kb on - strand at 45.237 kb on - strand, within GFF4259 at 45.405 kb on + strand, within GFF4259 at 45.406 kb on - strand, within GFF4259 at 45.409 kb on + strand, within GFF4259 at 45.409 kb on + strand, within GFF4259 at 45.686 kb on - strand at 45.700 kb on + strand at 45.703 kb on + strand at 45.805 kb on + strand at 45.806 kb on - strand at 45.848 kb on + strand at 45.908 kb on + strand at 45.945 kb on + strand, within GFF4260
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 41,917 - GFF4257 0.19 -0.1 41,917 - GFF4257 0.19 +0.3 41,939 + GFF4257 0.21 +0.2 41,939 + GFF4257 0.21 +1.1 41,940 - GFF4257 0.21 -0.1 41,940 - GFF4257 0.21 +0.2 41,947 + GFF4257 0.22 -0.3 41,948 - GFF4257 0.22 +0.2 41,980 + GFF4257 0.25 +1.5 41,981 - GFF4257 0.25 +0.3 41,981 - GFF4257 0.25 +0.1 41,981 - GFF4257 0.25 -0.1 41,982 + GFF4257 0.26 +0.1 41,982 + GFF4257 0.26 +0.8 41,983 - GFF4257 0.26 -0.3 42,004 - GFF4257 0.28 +0.5 42,142 + GFF4257 0.41 -0.2 42,299 + GFF4257 0.57 -0.1 42,334 + GFF4257 0.61 +0.2 42,521 - GFF4257 0.79 -0.4 42,521 - GFF4257 0.79 +0.3 42,827 + -0.1 42,828 - -0.1 42,828 - -0.2 42,865 + -0.0 43,069 + -0.0 43,142 + GFF4258 0.12 -0.2 43,178 + GFF4258 0.14 +0.2 43,186 - GFF4258 0.14 -2.1 43,186 - GFF4258 0.14 +0.0 43,207 + GFF4258 0.15 +0.1 43,223 + GFF4258 0.16 -0.9 43,427 - GFF4258 0.26 +0.2 43,585 + GFF4258 0.33 -0.2 43,609 + GFF4258 0.34 +0.3 43,685 - GFF4258 0.38 -0.9 43,731 - GFF4258 0.40 +0.0 43,769 - GFF4258 0.42 -0.6 43,853 + GFF4258 0.46 -0.6 44,020 + GFF4258 0.54 -0.5 44,020 + GFF4258 0.54 -0.2 44,024 - GFF4258 0.54 -0.2 44,073 - GFF4258 0.56 +0.1 44,095 + GFF4258 0.57 -0.6 44,096 - GFF4258 0.58 -0.6 44,103 - GFF4258 0.58 +0.3 44,103 - GFF4258 0.58 +0.1 44,104 + GFF4258 0.58 +0.9 44,141 + GFF4258 0.60 -0.2 44,142 - GFF4258 0.60 -0.1 44,179 + GFF4258 0.62 -0.2 44,465 - GFF4258 0.75 -0.0 44,501 - GFF4258 0.77 -0.5 44,536 + GFF4258 0.79 +0.3 44,536 + GFF4258 0.79 +0.0 44,598 - GFF4258 0.82 -0.5 44,600 - GFF4258 0.82 +0.3 44,603 + GFF4258 0.82 -0.1 44,603 + GFF4258 0.82 -0.8 44,603 + GFF4258 0.82 +0.0 44,603 + GFF4258 0.82 -0.3 44,603 + GFF4258 0.82 +0.6 44,604 - GFF4258 0.82 +0.2 44,605 + GFF4258 0.82 +0.1 44,605 + GFF4258 0.82 -0.3 44,618 + GFF4258 0.82 -0.2 44,696 + GFF4258 0.86 -0.7 44,783 - +0.8 44,801 + +0.6 44,801 + -0.6 44,802 - -0.1 44,803 + +0.1 44,804 - +0.9 44,804 - -1.0 44,823 + +0.6 44,829 - -0.4 44,850 + +0.4 44,927 + -0.7 44,928 - +0.5 44,928 - -1.5 44,958 + -0.1 44,985 - +0.4 45,047 - -0.3 45,237 - GFF4259 0.34 +0.0 45,405 + GFF4259 0.60 +0.2 45,406 - GFF4259 0.60 -0.4 45,409 + GFF4259 0.61 -0.3 45,409 + GFF4259 0.61 +0.4 45,686 - +0.7 45,700 + +0.1 45,703 + -0.1 45,805 + -0.1 45,806 - -0.4 45,848 + +0.0 45,908 + +0.4 45,945 + GFF4260 0.21 -0.3
Or see this region's nucleotide sequence