Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4048

Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4047 and GFF4117 are separated by 133 nucleotidesGFF4117 and GFF4048 are separated by 321 nucleotidesGFF4048 and GFF4049 are separated by 16 nucleotides GFF4047 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 8,803 to 9,543 GFF4047 GFF4117 - tRNA-Asp-GTC, at 9,677 to 9,753 GFF4117 GFF4048 - Uncharacterized protein ImpA, at 10,075 to 11,124 GFF4048 GFF4049 - Uncharacterized protein ImpH/VasB, at 11,141 to 12,136 GFF4049 Position (kb) 10 11 12Strain fitness (log2 ratio) -2 -1 0 1at 9.884 kb on + strandat 9.906 kb on + strandat 9.957 kb on + strandat 10.043 kb on + strandat 10.417 kb on + strand, within GFF4048at 10.686 kb on + strand, within GFF4048at 10.686 kb on + strand, within GFF4048at 10.927 kb on - strand, within GFF4048at 11.012 kb on + strand, within GFF4048at 11.137 kb on - strandat 11.320 kb on + strand, within GFF4049at 11.402 kb on - strand, within GFF4049at 11.555 kb on + strand, within GFF4049at 11.566 kb on - strand, within GFF4049at 11.703 kb on + strand, within GFF4049at 11.916 kb on + strand, within GFF4049at 11.917 kb on - strand, within GFF4049at 12.047 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI
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9,884 + -0.5
9,906 + +1.0
9,957 + -0.1
10,043 + +1.1
10,417 + GFF4048 0.33 -0.4
10,686 + GFF4048 0.58 +0.2
10,686 + GFF4048 0.58 +0.1
10,927 - GFF4048 0.81 -0.8
11,012 + GFF4048 0.89 -1.1
11,137 - -0.3
11,320 + GFF4049 0.18 +0.1
11,402 - GFF4049 0.26 +0.6
11,555 + GFF4049 0.42 +1.2
11,566 - GFF4049 0.43 +0.2
11,703 + GFF4049 0.56 -0.1
11,916 + GFF4049 0.78 +0.2
11,917 - GFF4049 0.78 -1.8
12,047 - +1.5

Or see this region's nucleotide sequence