Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4041 and GFF4042 are separated by 77 nucleotides GFF4042 and GFF4043 are separated by 55 nucleotides GFF4043 and GFF4044 are separated by 218 nucleotides
GFF4041 - FIG00613320: hypothetical protein, at 3,629 to 4,429
GFF4041
GFF4042 - SAM-dependent methyltransferase YafE (UbiE paralog), at 4,507 to 5,277
GFF4042
GFF4043 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,333 to 6,553
GFF4043
GFF4044 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 6,772 to 7,527
GFF4044
Position (kb) 5
6
7 Strain fitness (log2 ratio) -1
0
1 at 4.338 kb on + strand, within GFF4041 at 4.479 kb on - strand at 4.571 kb on + strand at 4.572 kb on - strand at 4.572 kb on - strand at 4.779 kb on - strand, within GFF4042 at 4.782 kb on + strand, within GFF4042 at 4.785 kb on + strand, within GFF4042 at 4.805 kb on - strand, within GFF4042 at 5.025 kb on + strand, within GFF4042 at 5.029 kb on + strand, within GFF4042 at 5.029 kb on + strand, within GFF4042 at 5.030 kb on - strand, within GFF4042 at 5.139 kb on + strand, within GFF4042 at 5.247 kb on + strand at 5.247 kb on + strand at 5.276 kb on - strand at 5.332 kb on - strand at 5.350 kb on + strand at 5.372 kb on - strand at 5.391 kb on - strand at 5.401 kb on + strand at 5.487 kb on + strand, within GFF4043 at 5.492 kb on - strand, within GFF4043 at 5.665 kb on - strand, within GFF4043 at 5.665 kb on - strand, within GFF4043 at 5.665 kb on - strand, within GFF4043 at 5.687 kb on - strand, within GFF4043 at 5.777 kb on - strand, within GFF4043 at 5.777 kb on - strand, within GFF4043 at 5.782 kb on - strand, within GFF4043 at 5.782 kb on - strand, within GFF4043 at 5.849 kb on - strand, within GFF4043 at 5.945 kb on + strand, within GFF4043 at 5.995 kb on + strand, within GFF4043 at 5.996 kb on - strand, within GFF4043 at 6.107 kb on - strand, within GFF4043 at 6.171 kb on - strand, within GFF4043 at 6.218 kb on - strand, within GFF4043 at 6.231 kb on - strand, within GFF4043 at 6.333 kb on - strand, within GFF4043 at 6.356 kb on - strand, within GFF4043 at 6.384 kb on + strand, within GFF4043 at 6.385 kb on - strand, within GFF4043 at 6.385 kb on - strand, within GFF4043 at 6.388 kb on + strand, within GFF4043 at 6.389 kb on - strand, within GFF4043 at 6.389 kb on - strand, within GFF4043 at 6.389 kb on - strand, within GFF4043 at 6.391 kb on - strand, within GFF4043 at 6.411 kb on - strand, within GFF4043 at 6.424 kb on - strand, within GFF4043 at 6.424 kb on - strand, within GFF4043 at 6.431 kb on - strand, within GFF4043 at 6.431 kb on - strand, within GFF4043 at 6.442 kb on - strand at 6.536 kb on - strand at 6.681 kb on - strand at 6.721 kb on - strand at 6.721 kb on - strand at 6.763 kb on + strand at 6.952 kb on - strand, within GFF4044 at 7.017 kb on + strand, within GFF4044 at 7.017 kb on + strand, within GFF4044 at 7.018 kb on - strand, within GFF4044 at 7.022 kb on - strand, within GFF4044 at 7.245 kb on + strand, within GFF4044 at 7.246 kb on - strand, within GFF4044 at 7.401 kb on + strand, within GFF4044 at 7.402 kb on - strand, within GFF4044 at 7.402 kb on - strand, within GFF4044 at 7.513 kb on - strand at 7.527 kb on + strand at 7.528 kb on - strand at 7.530 kb on - strand at 7.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 4,338 + GFF4041 0.89 -0.0 4,479 - -0.0 4,571 + -0.2 4,572 - -0.6 4,572 - -0.9 4,779 - GFF4042 0.35 -1.6 4,782 + GFF4042 0.36 -0.1 4,785 + GFF4042 0.36 +0.4 4,805 - GFF4042 0.39 +0.2 5,025 + GFF4042 0.67 +0.4 5,029 + GFF4042 0.68 -0.3 5,029 + GFF4042 0.68 +1.5 5,030 - GFF4042 0.68 +0.2 5,139 + GFF4042 0.82 -0.2 5,247 + +0.1 5,247 + +1.5 5,276 - -0.6 5,332 - +0.4 5,350 + -0.8 5,372 - -0.5 5,391 - -0.2 5,401 + -0.4 5,487 + GFF4043 0.13 +0.3 5,492 - GFF4043 0.13 +0.3 5,665 - GFF4043 0.27 +0.4 5,665 - GFF4043 0.27 -1.0 5,665 - GFF4043 0.27 +0.2 5,687 - GFF4043 0.29 -0.6 5,777 - GFF4043 0.36 -0.8 5,777 - GFF4043 0.36 +1.0 5,782 - GFF4043 0.37 -0.1 5,782 - GFF4043 0.37 +0.2 5,849 - GFF4043 0.42 +0.2 5,945 + GFF4043 0.50 +0.3 5,995 + GFF4043 0.54 +0.8 5,996 - GFF4043 0.54 +0.2 6,107 - GFF4043 0.63 -0.6 6,171 - GFF4043 0.69 -0.1 6,218 - GFF4043 0.72 -1.3 6,231 - GFF4043 0.74 -0.5 6,333 - GFF4043 0.82 -0.7 6,356 - GFF4043 0.84 +0.8 6,384 + GFF4043 0.86 -0.4 6,385 - GFF4043 0.86 -1.1 6,385 - GFF4043 0.86 -1.6 6,388 + GFF4043 0.86 +0.0 6,389 - GFF4043 0.86 -0.2 6,389 - GFF4043 0.86 -0.0 6,389 - GFF4043 0.86 -0.4 6,391 - GFF4043 0.87 -0.2 6,411 - GFF4043 0.88 -0.1 6,424 - GFF4043 0.89 +0.2 6,424 - GFF4043 0.89 -0.2 6,431 - GFF4043 0.90 -0.2 6,431 - GFF4043 0.90 +0.2 6,442 - +0.2 6,536 - -0.2 6,681 - +0.3 6,721 - -1.1 6,721 - -0.9 6,763 + +0.1 6,952 - GFF4044 0.24 -0.3 7,017 + GFF4044 0.32 +0.4 7,017 + GFF4044 0.32 +0.3 7,018 - GFF4044 0.33 -0.2 7,022 - GFF4044 0.33 +0.1 7,245 + GFF4044 0.63 -0.0 7,246 - GFF4044 0.63 +0.9 7,401 + GFF4044 0.83 +0.2 7,402 - GFF4044 0.83 -0.3 7,402 - GFF4044 0.83 +0.5 7,513 - -0.3 7,527 + +0.5 7,528 - -1.4 7,530 - -0.6 7,532 + +0.6
Or see this region's nucleotide sequence