Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3879 and GFF3880 are separated by 110 nucleotides GFF3880 and GFF3881 are separated by 53 nucleotides
GFF3879 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 154,916 to 156,352
GFF3879
GFF3880 - Phosphomannomutase (EC 5.4.2.8), at 156,463 to 157,833
GFF3880
GFF3881 - Colanic acid biosynthsis UDP-glucose lipid carrier transferase WcaJ, at 157,887 to 159,281
GFF3881
Position (kb) 156
157
158 Strain fitness (log2 ratio) -1
0
1 at 155.552 kb on + strand, within GFF3879 at 155.855 kb on + strand, within GFF3879 at 156.023 kb on + strand, within GFF3879 at 156.082 kb on + strand, within GFF3879 at 156.191 kb on - strand, within GFF3879 at 156.218 kb on + strand at 156.276 kb on + strand at 156.276 kb on + strand at 156.435 kb on + strand at 156.490 kb on + strand at 156.681 kb on + strand, within GFF3880 at 156.682 kb on - strand, within GFF3880 at 156.772 kb on + strand, within GFF3880 at 156.773 kb on - strand, within GFF3880 at 156.898 kb on + strand, within GFF3880 at 156.899 kb on - strand, within GFF3880 at 156.925 kb on + strand, within GFF3880 at 157.255 kb on + strand, within GFF3880 at 157.256 kb on - strand, within GFF3880 at 157.462 kb on + strand, within GFF3880 at 157.961 kb on + strand at 158.022 kb on + strand at 158.022 kb on + strand at 158.022 kb on + strand at 158.023 kb on - strand at 158.023 kb on - strand at 158.233 kb on - strand, within GFF3881 at 158.311 kb on - strand, within GFF3881 at 158.313 kb on - strand, within GFF3881 at 158.671 kb on - strand, within GFF3881
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 155,552 + GFF3879 0.44 -0.8 155,855 + GFF3879 0.65 +1.0 156,023 + GFF3879 0.77 -0.2 156,082 + GFF3879 0.81 +0.0 156,191 - GFF3879 0.89 +0.3 156,218 + -0.2 156,276 + +0.3 156,276 + -0.7 156,435 + -0.2 156,490 + +1.5 156,681 + GFF3880 0.16 +0.4 156,682 - GFF3880 0.16 +1.0 156,772 + GFF3880 0.23 -0.4 156,773 - GFF3880 0.23 +0.9 156,898 + GFF3880 0.32 +0.1 156,899 - GFF3880 0.32 -0.0 156,925 + GFF3880 0.34 -0.3 157,255 + GFF3880 0.58 +0.2 157,256 - GFF3880 0.58 -0.1 157,462 + GFF3880 0.73 +0.4 157,961 + -0.2 158,022 + -0.2 158,022 + +0.1 158,022 + +0.2 158,023 - -0.2 158,023 - +0.6 158,233 - GFF3881 0.25 +0.5 158,311 - GFF3881 0.30 -0.5 158,313 - GFF3881 0.31 +0.3 158,671 - GFF3881 0.56 -1.6
Or see this region's nucleotide sequence