Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3394 and GFF3395 are separated by 241 nucleotides GFF3395 and GFF3396 are separated by 96 nucleotides GFF3396 and GFF3397 are separated by 147 nucleotides
GFF3394 - putative transcriptional repressor, at 158,603 to 159,238
GFF3394
GFF3395 - Putative outer membrane protein, at 159,480 to 159,737
GFF3395
GFF3396 - L,D-transpeptidase YcfS, at 159,834 to 160,799
GFF3396
GFF3397 - Transcription-repair coupling factor, at 160,947 to 164,393
GFF3397
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1 at 158.876 kb on - strand, within GFF3394 at 158.885 kb on - strand, within GFF3394 at 158.940 kb on + strand, within GFF3394 at 158.943 kb on - strand, within GFF3394 at 159.018 kb on + strand, within GFF3394 at 159.101 kb on + strand, within GFF3394 at 159.402 kb on + strand at 159.403 kb on - strand at 159.680 kb on + strand, within GFF3395 at 159.729 kb on + strand at 159.729 kb on + strand at 159.732 kb on - strand at 159.735 kb on + strand at 159.736 kb on - strand at 159.736 kb on - strand at 159.836 kb on - strand at 160.002 kb on - strand, within GFF3396 at 160.007 kb on + strand, within GFF3396 at 160.125 kb on + strand, within GFF3396 at 160.126 kb on - strand, within GFF3396 at 160.126 kb on - strand, within GFF3396 at 160.169 kb on + strand, within GFF3396 at 160.460 kb on - strand, within GFF3396 at 160.460 kb on - strand, within GFF3396 at 160.460 kb on - strand, within GFF3396 at 160.490 kb on + strand, within GFF3396 at 160.561 kb on + strand, within GFF3396 at 160.562 kb on - strand, within GFF3396 at 160.713 kb on - strand at 160.788 kb on - strand at 160.788 kb on - strand at 160.801 kb on - strand at 160.848 kb on + strand at 160.999 kb on - strand at 160.999 kb on - strand at 160.999 kb on - strand at 161.313 kb on - strand, within GFF3397 at 161.333 kb on + strand, within GFF3397 at 161.470 kb on + strand, within GFF3397 at 161.471 kb on - strand, within GFF3397 at 161.471 kb on - strand, within GFF3397 at 161.578 kb on - strand, within GFF3397 at 161.789 kb on + strand, within GFF3397
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 158,876 - GFF3394 0.43 +0.5 158,885 - GFF3394 0.44 -0.4 158,940 + GFF3394 0.53 -0.6 158,943 - GFF3394 0.53 +0.6 159,018 + GFF3394 0.65 -0.7 159,101 + GFF3394 0.78 +0.4 159,402 + +0.4 159,403 - -0.4 159,680 + GFF3395 0.78 -0.1 159,729 + -0.7 159,729 + +0.2 159,732 - +1.1 159,735 + +0.1 159,736 - +0.6 159,736 - +0.0 159,836 - -0.2 160,002 - GFF3396 0.17 -0.1 160,007 + GFF3396 0.18 -0.1 160,125 + GFF3396 0.30 +0.0 160,126 - GFF3396 0.30 -0.2 160,126 - GFF3396 0.30 +1.1 160,169 + GFF3396 0.35 +0.3 160,460 - GFF3396 0.65 +1.7 160,460 - GFF3396 0.65 -0.1 160,460 - GFF3396 0.65 +0.1 160,490 + GFF3396 0.68 +0.1 160,561 + GFF3396 0.75 +0.8 160,562 - GFF3396 0.75 +0.5 160,713 - -0.0 160,788 - +0.2 160,788 - -0.0 160,801 - +0.1 160,848 + +0.4 160,999 - +0.4 160,999 - +0.2 160,999 - -0.8 161,313 - GFF3397 0.11 +0.9 161,333 + GFF3397 0.11 -0.5 161,470 + GFF3397 0.15 -2.0 161,471 - GFF3397 0.15 +0.1 161,471 - GFF3397 0.15 +0.5 161,578 - GFF3397 0.18 -0.1 161,789 + GFF3397 0.24 -0.8
Or see this region's nucleotide sequence