Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3207 and GFF3208 are separated by 33 nucleotides GFF3208 and GFF3209 are separated by 65 nucleotides
GFF3207 - 2-ketobutyrate formate-lyase (EC 2.3.1.-) / Pyruvate formate-lyase (EC 2.3.1.54), at 211,567 to 213,861
GFF3207
GFF3208 - Acetate kinase (EC 2.7.2.1), at 213,895 to 215,103
GFF3208
GFF3209 - L-threonine transporter, anaerobically inducible, at 215,169 to 216,500
GFF3209
Position (kb) 213
214
215
216 Strain fitness (log2 ratio) -1
0
1
2
3 at 212.990 kb on + strand, within GFF3207 at 212.991 kb on - strand, within GFF3207 at 212.994 kb on + strand, within GFF3207 at 212.994 kb on + strand, within GFF3207 at 212.995 kb on - strand, within GFF3207 at 213.071 kb on + strand, within GFF3207 at 213.333 kb on - strand, within GFF3207 at 213.333 kb on - strand, within GFF3207 at 213.403 kb on + strand, within GFF3207 at 213.414 kb on + strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.473 kb on + strand, within GFF3207 at 213.665 kb on - strand at 213.749 kb on + strand at 213.753 kb on + strand at 213.754 kb on - strand at 213.828 kb on - strand at 214.072 kb on + strand, within GFF3208 at 214.159 kb on + strand, within GFF3208 at 214.160 kb on - strand, within GFF3208 at 214.443 kb on + strand, within GFF3208 at 214.637 kb on - strand, within GFF3208 at 214.807 kb on - strand, within GFF3208 at 214.807 kb on - strand, within GFF3208 at 214.826 kb on - strand, within GFF3208 at 214.826 kb on - strand, within GFF3208 at 215.003 kb on - strand at 215.105 kb on + strand at 215.157 kb on + strand at 215.157 kb on + strand at 215.271 kb on - strand at 215.368 kb on - strand, within GFF3209 at 215.411 kb on + strand, within GFF3209 at 215.448 kb on - strand, within GFF3209 at 215.551 kb on + strand, within GFF3209 at 215.684 kb on + strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.750 kb on + strand, within GFF3209 at 215.786 kb on - strand, within GFF3209 at 215.799 kb on - strand, within GFF3209 at 215.997 kb on + strand, within GFF3209 at 216.004 kb on + strand, within GFF3209
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 212,990 + GFF3207 0.62 -0.4 212,991 - GFF3207 0.62 -0.1 212,994 + GFF3207 0.62 +3.0 212,994 + GFF3207 0.62 +0.4 212,995 - GFF3207 0.62 -0.2 213,071 + GFF3207 0.66 +0.6 213,333 - GFF3207 0.77 -0.5 213,333 - GFF3207 0.77 -0.2 213,403 + GFF3207 0.80 -0.7 213,414 + GFF3207 0.80 +0.5 213,442 - GFF3207 0.82 +0.9 213,442 - GFF3207 0.82 -0.1 213,442 - GFF3207 0.82 +0.1 213,442 - GFF3207 0.82 -0.5 213,473 + GFF3207 0.83 -0.5 213,665 - +0.3 213,749 + +0.2 213,753 + -0.1 213,754 - +0.3 213,828 - +0.0 214,072 + GFF3208 0.15 +1.1 214,159 + GFF3208 0.22 +0.3 214,160 - GFF3208 0.22 -0.4 214,443 + GFF3208 0.45 +0.2 214,637 - GFF3208 0.61 +0.5 214,807 - GFF3208 0.75 +0.3 214,807 - GFF3208 0.75 +0.2 214,826 - GFF3208 0.77 +0.0 214,826 - GFF3208 0.77 +0.6 215,003 - +1.3 215,105 + +0.3 215,157 + +0.5 215,157 + -0.3 215,271 - -0.3 215,368 - GFF3209 0.15 -0.4 215,411 + GFF3209 0.18 +0.5 215,448 - GFF3209 0.21 -0.1 215,551 + GFF3209 0.29 -0.8 215,684 + GFF3209 0.39 -0.1 215,721 - GFF3209 0.41 +0.1 215,721 - GFF3209 0.41 +0.1 215,721 - GFF3209 0.41 +0.1 215,750 + GFF3209 0.44 +0.0 215,786 - GFF3209 0.46 -0.0 215,799 - GFF3209 0.47 +0.5 215,997 + GFF3209 0.62 -0.2 216,004 + GFF3209 0.63 +0.3
Or see this region's nucleotide sequence