Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3124 and GFF3125 are separated by 105 nucleotides GFF3125 and GFF3126 are separated by 165 nucleotides
GFF3124 - NADPH-dependent broad range aldehyde dehydrogenase YqhD, at 129,272 to 130,435
GFF3124
GFF3125 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconate reductase A (EC 1.1.1.274), at 130,541 to 131,368
GFF3125
GFF3126 - 2-oxoglutarate/malate translocator, at 131,534 to 133,018
GFF3126
Position (kb) 130
131
132 Strain fitness (log2 ratio) -1
0
1 at 129.599 kb on + strand, within GFF3124 at 129.807 kb on - strand, within GFF3124 at 129.814 kb on + strand, within GFF3124 at 129.814 kb on + strand, within GFF3124 at 129.815 kb on - strand, within GFF3124 at 129.815 kb on - strand, within GFF3124 at 129.815 kb on - strand, within GFF3124 at 129.866 kb on - strand, within GFF3124 at 129.878 kb on + strand, within GFF3124 at 129.879 kb on - strand, within GFF3124 at 129.933 kb on + strand, within GFF3124 at 129.984 kb on - strand, within GFF3124 at 130.015 kb on + strand, within GFF3124 at 130.072 kb on + strand, within GFF3124 at 130.072 kb on + strand, within GFF3124 at 130.168 kb on + strand, within GFF3124 at 130.190 kb on + strand, within GFF3124 at 130.190 kb on + strand, within GFF3124 at 130.191 kb on - strand, within GFF3124 at 130.237 kb on + strand, within GFF3124 at 130.238 kb on - strand, within GFF3124 at 130.292 kb on - strand, within GFF3124 at 130.482 kb on + strand at 130.482 kb on + strand at 130.483 kb on - strand at 130.524 kb on + strand at 130.581 kb on + strand at 130.582 kb on - strand at 130.839 kb on - strand, within GFF3125 at 130.839 kb on - strand, within GFF3125 at 130.936 kb on - strand, within GFF3125 at 130.954 kb on + strand, within GFF3125 at 131.055 kb on + strand, within GFF3125 at 131.128 kb on - strand, within GFF3125 at 131.451 kb on + strand at 131.459 kb on + strand at 131.546 kb on + strand at 131.734 kb on + strand, within GFF3126 at 131.752 kb on + strand, within GFF3126 at 131.781 kb on - strand, within GFF3126 at 131.816 kb on - strand, within GFF3126 at 131.891 kb on - strand, within GFF3126 at 131.940 kb on + strand, within GFF3126 at 132.303 kb on - strand, within GFF3126 at 132.332 kb on - strand, within GFF3126
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 129,599 + GFF3124 0.28 -1.0 129,807 - GFF3124 0.46 +0.0 129,814 + GFF3124 0.47 +0.8 129,814 + GFF3124 0.47 -0.4 129,815 - GFF3124 0.47 +0.9 129,815 - GFF3124 0.47 -0.4 129,815 - GFF3124 0.47 +0.1 129,866 - GFF3124 0.51 -0.0 129,878 + GFF3124 0.52 +0.1 129,879 - GFF3124 0.52 +1.3 129,933 + GFF3124 0.57 +0.4 129,984 - GFF3124 0.61 +0.2 130,015 + GFF3124 0.64 -0.7 130,072 + GFF3124 0.69 -0.0 130,072 + GFF3124 0.69 +0.2 130,168 + GFF3124 0.77 -0.3 130,190 + GFF3124 0.79 +0.4 130,190 + GFF3124 0.79 +0.9 130,191 - GFF3124 0.79 +0.1 130,237 + GFF3124 0.83 +0.1 130,238 - GFF3124 0.83 +0.7 130,292 - GFF3124 0.88 -0.0 130,482 + -0.2 130,482 + +0.2 130,483 - +0.1 130,524 + +0.2 130,581 + +0.5 130,582 - -0.4 130,839 - GFF3125 0.36 +0.6 130,839 - GFF3125 0.36 +0.3 130,936 - GFF3125 0.48 +0.4 130,954 + GFF3125 0.50 -0.1 131,055 + GFF3125 0.62 -0.4 131,128 - GFF3125 0.71 +0.9 131,451 + +0.2 131,459 + -0.2 131,546 + +0.0 131,734 + GFF3126 0.13 -0.7 131,752 + GFF3126 0.15 -0.0 131,781 - GFF3126 0.17 +0.0 131,816 - GFF3126 0.19 +1.6 131,891 - GFF3126 0.24 +0.5 131,940 + GFF3126 0.27 -0.5 132,303 - GFF3126 0.52 -0.4 132,332 - GFF3126 0.54 +0.0
Or see this region's nucleotide sequence