Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2897 and GFF2898 are separated by 2 nucleotides GFF2898 and GFF2899 overlap by 4 nucleotides GFF2899 and GFF2900 are separated by 283 nucleotides
GFF2897 - HTH-type transcriptional regulator IlvY, at 23,977 to 24,864
GFF2897
GFF2898 - FIG074102: hypothetical protein, at 24,867 to 25,208
GFF2898
GFF2899 - Phage-related protein, at 25,205 to 25,522
GFF2899
GFF2900 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 25,806 to 27,350
GFF2900
Position (kb) 25
26 Strain fitness (log2 ratio) -1
0
1 at 24.258 kb on + strand, within GFF2897 at 24.283 kb on + strand, within GFF2897 at 24.635 kb on - strand, within GFF2897 at 24.876 kb on + strand at 24.915 kb on - strand, within GFF2898 at 24.987 kb on - strand, within GFF2898 at 24.987 kb on - strand, within GFF2898 at 24.999 kb on - strand, within GFF2898 at 25.056 kb on + strand, within GFF2898 at 25.057 kb on - strand, within GFF2898 at 25.057 kb on - strand, within GFF2898 at 25.130 kb on + strand, within GFF2898 at 25.240 kb on + strand, within GFF2899 at 25.304 kb on - strand, within GFF2899 at 25.341 kb on + strand, within GFF2899 at 25.341 kb on + strand, within GFF2899 at 25.342 kb on - strand, within GFF2899 at 25.342 kb on - strand, within GFF2899 at 25.346 kb on - strand, within GFF2899 at 25.358 kb on - strand, within GFF2899 at 25.484 kb on + strand, within GFF2899 at 25.568 kb on - strand at 25.635 kb on + strand at 25.636 kb on - strand at 25.749 kb on + strand at 25.995 kb on - strand, within GFF2900 at 25.995 kb on - strand, within GFF2900 at 26.042 kb on + strand, within GFF2900 at 26.044 kb on + strand, within GFF2900 at 26.044 kb on + strand, within GFF2900 at 26.045 kb on - strand, within GFF2900 at 26.059 kb on - strand, within GFF2900 at 26.089 kb on + strand, within GFF2900 at 26.090 kb on - strand, within GFF2900 at 26.138 kb on + strand, within GFF2900 at 26.139 kb on - strand, within GFF2900 at 26.375 kb on - strand, within GFF2900 at 26.456 kb on - strand, within GFF2900
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 24,258 + GFF2897 0.32 +0.1 24,283 + GFF2897 0.34 +0.3 24,635 - GFF2897 0.74 -0.2 24,876 + -0.1 24,915 - GFF2898 0.14 +0.4 24,987 - GFF2898 0.35 +0.6 24,987 - GFF2898 0.35 -0.4 24,999 - GFF2898 0.39 +0.5 25,056 + GFF2898 0.55 +0.4 25,057 - GFF2898 0.56 -0.5 25,057 - GFF2898 0.56 -0.4 25,130 + GFF2898 0.77 -0.2 25,240 + GFF2899 0.11 -0.1 25,304 - GFF2899 0.31 +0.5 25,341 + GFF2899 0.43 +0.2 25,341 + GFF2899 0.43 +0.6 25,342 - GFF2899 0.43 +1.3 25,342 - GFF2899 0.43 -0.3 25,346 - GFF2899 0.44 +0.5 25,358 - GFF2899 0.48 -0.2 25,484 + GFF2899 0.88 +0.2 25,568 - -0.4 25,635 + +0.2 25,636 - -0.3 25,749 + +0.0 25,995 - GFF2900 0.12 +0.1 25,995 - GFF2900 0.12 +0.1 26,042 + GFF2900 0.15 +0.2 26,044 + GFF2900 0.15 -0.0 26,044 + GFF2900 0.15 +0.1 26,045 - GFF2900 0.15 -0.5 26,059 - GFF2900 0.16 +0.5 26,089 + GFF2900 0.18 -0.4 26,090 - GFF2900 0.18 +1.0 26,138 + GFF2900 0.21 +0.2 26,139 - GFF2900 0.22 +0.4 26,375 - GFF2900 0.37 +0.2 26,456 - GFF2900 0.42 -0.2
Or see this region's nucleotide sequence