Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2863 and GFF2864 are separated by 589 nucleotides GFF2864 and GFF2865 overlap by 1 nucleotides GFF2865 and GFF2866 are separated by 162 nucleotides GFF2866 and GFF2867 are separated by 485 nucleotides
GFF2863 - FIG074102: hypothetical protein, at 381,244 to 381,390
GFF2863
GFF2864 - Mobile element protein, at 381,980 to 382,126
GFF2864
GFF2865 - Mobile element protein, at 382,126 to 382,983
GFF2865
GFF2866 - Phage integrase, at 383,146 to 383,475
GFF2866
GFF2867 - hypothetical protein, at 383,961 to 384,161
GFF2867
Position (kb) 382
383 Strain fitness (log2 ratio) -1
0
1 at 381.216 kb on - strand at 381.217 kb on + strand at 381.217 kb on + strand at 381.351 kb on - strand, within GFF2863 at 381.377 kb on + strand at 381.464 kb on + strand at 381.601 kb on + strand at 381.616 kb on + strand at 381.640 kb on - strand at 381.713 kb on - strand at 381.790 kb on + strand at 382.000 kb on - strand, within GFF2864 at 382.491 kb on + strand, within GFF2865 at 382.491 kb on + strand, within GFF2865 at 382.494 kb on - strand, within GFF2865 at 382.494 kb on - strand, within GFF2865 at 382.574 kb on - strand, within GFF2865 at 382.601 kb on + strand, within GFF2865 at 382.660 kb on + strand, within GFF2865 at 382.705 kb on - strand, within GFF2865 at 382.942 kb on - strand at 383.083 kb on + strand at 383.084 kb on - strand at 383.410 kb on - strand, within GFF2866 at 383.410 kb on - strand, within GFF2866 at 383.876 kb on - strand at 383.934 kb on + strand at 383.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 381,216 - -0.2 381,217 + -0.2 381,217 + +0.6 381,351 - GFF2863 0.73 +0.3 381,377 + +1.5 381,464 + -0.9 381,601 + -0.4 381,616 + +1.5 381,640 - -0.8 381,713 - -1.1 381,790 + +0.1 382,000 - GFF2864 0.14 +0.2 382,491 + GFF2865 0.43 -0.7 382,491 + GFF2865 0.43 +0.5 382,494 - GFF2865 0.43 -0.1 382,494 - GFF2865 0.43 -0.2 382,574 - GFF2865 0.52 -0.3 382,601 + GFF2865 0.55 -0.2 382,660 + GFF2865 0.62 +0.4 382,705 - GFF2865 0.67 -0.6 382,942 - -0.5 383,083 + -1.0 383,084 - +0.4 383,410 - GFF2866 0.80 -0.4 383,410 - GFF2866 0.80 +1.1 383,876 - -0.1 383,934 + +0.4 383,958 + +0.3
Or see this region's nucleotide sequence