Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2846 and GFF2847 are separated by 97 nucleotides GFF2847 and GFF2848 are separated by 128 nucleotides GFF2848 and GFF2849 overlap by 32 nucleotides
GFF2846 - ProQ: influences osmotic activation of compatible solute ProP, at 368,474 to 369,160
GFF2846
GFF2847 - Free methionine-(R)-sulfoxide reductase, contains GAF domain, at 369,258 to 369,755
GFF2847
GFF2848 - Paraquat-inducible protein A, at 369,884 to 371,167
GFF2848
GFF2849 - Paraquat-inducible protein B, at 371,136 to 373,769
GFF2849
Position (kb) 369
370
371
372 Strain fitness (log2 ratio) -1
0
1 at 368.939 kb on - strand, within GFF2846 at 368.946 kb on + strand, within GFF2846 at 368.947 kb on - strand, within GFF2846 at 368.968 kb on + strand, within GFF2846 at 368.971 kb on - strand, within GFF2846 at 368.979 kb on - strand, within GFF2846 at 368.979 kb on - strand, within GFF2846 at 369.137 kb on - strand at 369.146 kb on - strand at 369.174 kb on + strand at 369.220 kb on - strand at 369.221 kb on + strand at 369.222 kb on - strand at 369.236 kb on + strand at 369.244 kb on - strand at 369.244 kb on - strand at 369.380 kb on + strand, within GFF2847 at 369.385 kb on - strand, within GFF2847 at 369.385 kb on - strand, within GFF2847 at 369.464 kb on + strand, within GFF2847 at 369.494 kb on + strand, within GFF2847 at 369.614 kb on - strand, within GFF2847 at 369.654 kb on + strand, within GFF2847 at 369.726 kb on + strand at 369.737 kb on - strand at 369.737 kb on - strand at 369.739 kb on - strand at 369.757 kb on - strand at 369.757 kb on - strand at 369.777 kb on + strand at 369.798 kb on - strand at 369.809 kb on + strand at 369.853 kb on + strand at 369.858 kb on + strand at 370.031 kb on + strand, within GFF2848 at 370.032 kb on - strand, within GFF2848 at 370.116 kb on + strand, within GFF2848 at 370.153 kb on + strand, within GFF2848 at 370.157 kb on + strand, within GFF2848 at 370.396 kb on + strand, within GFF2848 at 370.428 kb on - strand, within GFF2848 at 370.483 kb on + strand, within GFF2848 at 370.483 kb on + strand, within GFF2848 at 370.671 kb on + strand, within GFF2848 at 370.671 kb on + strand, within GFF2848 at 370.749 kb on - strand, within GFF2848 at 370.777 kb on + strand, within GFF2848 at 370.847 kb on - strand, within GFF2848 at 370.882 kb on + strand, within GFF2848 at 370.882 kb on + strand, within GFF2848 at 370.906 kb on + strand, within GFF2848 at 371.017 kb on + strand, within GFF2848 at 371.041 kb on + strand at 371.249 kb on - strand at 371.259 kb on + strand at 371.294 kb on + strand at 371.325 kb on - strand at 371.415 kb on - strand, within GFF2849 at 371.526 kb on + strand, within GFF2849 at 371.526 kb on + strand, within GFF2849 at 371.604 kb on - strand, within GFF2849 at 371.674 kb on - strand, within GFF2849 at 371.703 kb on + strand, within GFF2849 at 371.709 kb on + strand, within GFF2849 at 371.828 kb on - strand, within GFF2849 at 371.945 kb on - strand, within GFF2849 at 371.946 kb on + strand, within GFF2849 at 371.949 kb on - strand, within GFF2849 at 371.956 kb on + strand, within GFF2849
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 368,939 - GFF2846 0.68 -0.4 368,946 + GFF2846 0.69 +1.6 368,947 - GFF2846 0.69 +0.3 368,968 + GFF2846 0.72 -0.7 368,971 - GFF2846 0.72 +0.9 368,979 - GFF2846 0.74 +0.4 368,979 - GFF2846 0.74 -0.0 369,137 - +0.2 369,146 - +0.6 369,174 + +0.9 369,220 - +0.2 369,221 + +0.6 369,222 - -0.3 369,236 + +1.1 369,244 - +0.1 369,244 - -0.0 369,380 + GFF2847 0.24 +0.6 369,385 - GFF2847 0.26 +1.1 369,385 - GFF2847 0.26 +1.5 369,464 + GFF2847 0.41 -0.7 369,494 + GFF2847 0.47 -0.3 369,614 - GFF2847 0.71 +0.4 369,654 + GFF2847 0.80 +0.2 369,726 + +0.4 369,737 - -0.3 369,737 - +0.1 369,739 - +0.5 369,757 - +0.5 369,757 - -0.2 369,777 + -0.3 369,798 - +0.4 369,809 + +0.5 369,853 + +0.2 369,858 + +0.6 370,031 + GFF2848 0.11 -1.4 370,032 - GFF2848 0.12 -0.2 370,116 + GFF2848 0.18 -1.4 370,153 + GFF2848 0.21 +0.1 370,157 + GFF2848 0.21 -0.5 370,396 + GFF2848 0.40 -0.6 370,428 - GFF2848 0.42 -0.7 370,483 + GFF2848 0.47 -0.1 370,483 + GFF2848 0.47 -0.0 370,671 + GFF2848 0.61 +0.2 370,671 + GFF2848 0.61 +0.7 370,749 - GFF2848 0.67 -0.1 370,777 + GFF2848 0.70 -0.1 370,847 - GFF2848 0.75 -0.2 370,882 + GFF2848 0.78 +0.1 370,882 + GFF2848 0.78 -0.2 370,906 + GFF2848 0.80 -0.5 371,017 + GFF2848 0.88 +0.4 371,041 + -0.5 371,249 - +0.2 371,259 + +0.5 371,294 + +0.1 371,325 - -0.1 371,415 - GFF2849 0.11 +0.2 371,526 + GFF2849 0.15 -0.7 371,526 + GFF2849 0.15 -0.1 371,604 - GFF2849 0.18 -0.2 371,674 - GFF2849 0.20 -1.0 371,703 + GFF2849 0.22 +0.1 371,709 + GFF2849 0.22 -0.2 371,828 - GFF2849 0.26 +0.2 371,945 - GFF2849 0.31 +0.1 371,946 + GFF2849 0.31 -0.1 371,949 - GFF2849 0.31 +0.3 371,956 + GFF2849 0.31 -0.2
Or see this region's nucleotide sequence