Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2511 and GFF2512 are separated by 72 nucleotides GFF2512 and GFF2513 overlap by 1 nucleotides GFF2513 and GFF2514 are separated by 223 nucleotides
GFF2511 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 26,800 to 28,188
GFF2511
GFF2512 - Glutaminase (EC 3.5.1.2), at 28,261 to 29,187
GFF2512
GFF2513 - FIG00637950: hypothetical protein, at 29,187 to 29,546
GFF2513
GFF2514 - FIG00638206: hypothetical protein, at 29,770 to 30,717
GFF2514
Position (kb) 29
30 Strain fitness (log2 ratio) -1
0
1 at 28.279 kb on - strand at 28.417 kb on + strand, within GFF2512 at 28.713 kb on + strand, within GFF2512 at 28.713 kb on + strand, within GFF2512 at 28.835 kb on - strand, within GFF2512 at 28.843 kb on + strand, within GFF2512 at 28.844 kb on - strand, within GFF2512 at 29.392 kb on + strand, within GFF2513 at 29.494 kb on - strand, within GFF2513 at 29.495 kb on + strand, within GFF2513 at 29.527 kb on + strand at 29.579 kb on - strand at 29.601 kb on - strand at 29.621 kb on + strand at 29.622 kb on - strand at 29.622 kb on - strand at 29.907 kb on + strand, within GFF2514 at 29.919 kb on + strand, within GFF2514 at 29.919 kb on + strand, within GFF2514 at 29.974 kb on - strand, within GFF2514 at 29.974 kb on - strand, within GFF2514 at 30.276 kb on + strand, within GFF2514 at 30.277 kb on - strand, within GFF2514 at 30.508 kb on - strand, within GFF2514
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 28,279 - +0.1 28,417 + GFF2512 0.17 -0.1 28,713 + GFF2512 0.49 +0.2 28,713 + GFF2512 0.49 -0.5 28,835 - GFF2512 0.62 +0.5 28,843 + GFF2512 0.63 +0.7 28,844 - GFF2512 0.63 +0.0 29,392 + GFF2513 0.57 +1.5 29,494 - GFF2513 0.85 +1.3 29,495 + GFF2513 0.86 -0.9 29,527 + -0.7 29,579 - +0.3 29,601 - -0.4 29,621 + -1.0 29,622 - -0.0 29,622 - -0.6 29,907 + GFF2514 0.14 -0.0 29,919 + GFF2514 0.16 +0.2 29,919 + GFF2514 0.16 +0.0 29,974 - GFF2514 0.22 +0.2 29,974 - GFF2514 0.22 +0.3 30,276 + GFF2514 0.53 +1.7 30,277 - GFF2514 0.53 -0.2 30,508 - GFF2514 0.78 -0.1
Or see this region's nucleotide sequence