Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1866 and GFF1867 overlap by 4 nucleotides GFF1867 and GFF1868 overlap by 1 nucleotides GFF1868 and GFF1869 are separated by 14 nucleotides GFF1869 and GFF1870 overlap by 4 nucleotides
GFF1866 - O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26), at 58,066 to 59,433
GFF1866
GFF1867 - O-succinylbenzoate synthase (EC 4.2.1.113), at 59,430 to 60,392
GFF1867
GFF1868 - Naphthoate synthase (EC 4.1.3.36), at 60,392 to 61,249
GFF1868
GFF1869 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1- carboxylate synthase (EC 4.2.99.20), at 61,264 to 62,022
GFF1869
GFF1870 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase (EC 2.2.1.9), at 62,019 to 63,689
GFF1870
Position (kb) 60
61
62 Strain fitness (log2 ratio) -1
0
1 at 59.523 kb on - strand at 59.664 kb on - strand, within GFF1867 at 59.685 kb on - strand, within GFF1867 at 59.864 kb on - strand, within GFF1867 at 59.864 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.302 kb on - strand at 60.376 kb on - strand at 60.681 kb on + strand, within GFF1868 at 60.739 kb on + strand, within GFF1868 at 60.742 kb on - strand, within GFF1868 at 61.034 kb on - strand, within GFF1868 at 61.409 kb on - strand, within GFF1869 at 61.799 kb on + strand, within GFF1869 at 61.812 kb on + strand, within GFF1869 at 61.892 kb on + strand, within GFF1869 at 62.022 kb on + strand at 62.022 kb on + strand at 62.022 kb on + strand at 62.022 kb on + strand at 62.022 kb on + strand at 62.178 kb on - strand at 62.224 kb on + strand, within GFF1870 at 62.224 kb on + strand, within GFF1870 at 62.224 kb on + strand, within GFF1870
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 59,523 - -0.1 59,664 - GFF1867 0.24 +0.7 59,685 - GFF1867 0.26 +0.5 59,864 - GFF1867 0.45 -0.2 59,864 - GFF1867 0.45 -0.4 60,202 - GFF1867 0.80 +0.2 60,202 - GFF1867 0.80 +1.2 60,202 - GFF1867 0.80 -0.2 60,302 - -1.3 60,376 - +0.7 60,681 + GFF1868 0.34 -0.4 60,739 + GFF1868 0.40 -1.1 60,742 - GFF1868 0.41 -1.1 61,034 - GFF1868 0.75 -0.8 61,409 - GFF1869 0.19 +0.0 61,799 + GFF1869 0.70 +1.1 61,812 + GFF1869 0.72 +0.1 61,892 + GFF1869 0.83 +0.3 62,022 + -0.2 62,022 + -0.4 62,022 + -0.1 62,022 + +0.4 62,022 + +0.3 62,178 - +0.4 62,224 + GFF1870 0.12 +0.2 62,224 + GFF1870 0.12 -0.5 62,224 + GFF1870 0.12 -0.2
Or see this region's nucleotide sequence