Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1769 and GFF1770 overlap by 28 nucleotides GFF1770 and GFF1771 are separated by 93 nucleotides GFF1771 and GFF1772 overlap by 11 nucleotides
GFF1769 - Periplasmic protein torT precursor, at 73,468 to 74,508
GFF1769
GFF1770 - TorCAD operon transcriptional regulatory protein TorR, at 74,481 to 75,209
GFF1770
GFF1771 - Cytochrome c-type protein TorC, at 75,303 to 76,487
GFF1771
GFF1772 - Trimethylamine-N-oxide reductase (EC 1.6.6.9), at 76,477 to 79,029
GFF1772
Position (kb) 75
76
77 Strain fitness (log2 ratio) -1
0
1
2 at 74.358 kb on - strand, within GFF1769 at 74.358 kb on - strand, within GFF1769 at 74.413 kb on - strand at 74.480 kb on - strand at 74.480 kb on - strand at 74.549 kb on + strand at 74.556 kb on + strand, within GFF1770 at 74.556 kb on + strand, within GFF1770 at 74.557 kb on - strand, within GFF1770 at 74.601 kb on + strand, within GFF1770 at 74.601 kb on + strand, within GFF1770 at 74.679 kb on + strand, within GFF1770 at 74.680 kb on - strand, within GFF1770 at 74.705 kb on + strand, within GFF1770 at 74.768 kb on + strand, within GFF1770 at 74.768 kb on + strand, within GFF1770 at 74.768 kb on + strand, within GFF1770 at 74.769 kb on - strand, within GFF1770 at 74.770 kb on + strand, within GFF1770 at 74.878 kb on + strand, within GFF1770 at 74.945 kb on + strand, within GFF1770 at 75.113 kb on - strand, within GFF1770 at 75.209 kb on + strand at 75.458 kb on + strand, within GFF1771 at 75.458 kb on + strand, within GFF1771 at 75.461 kb on + strand, within GFF1771 at 75.461 kb on + strand, within GFF1771 at 75.461 kb on + strand, within GFF1771 at 75.475 kb on - strand, within GFF1771 at 75.484 kb on - strand, within GFF1771 at 75.485 kb on + strand, within GFF1771 at 75.486 kb on - strand, within GFF1771 at 75.558 kb on - strand, within GFF1771 at 75.558 kb on - strand, within GFF1771 at 75.578 kb on + strand, within GFF1771 at 75.579 kb on - strand, within GFF1771 at 75.702 kb on - strand, within GFF1771 at 75.732 kb on + strand, within GFF1771 at 75.732 kb on + strand, within GFF1771 at 75.732 kb on + strand, within GFF1771 at 75.733 kb on - strand, within GFF1771 at 75.733 kb on - strand, within GFF1771 at 75.733 kb on - strand, within GFF1771 at 75.909 kb on - strand, within GFF1771 at 75.927 kb on + strand, within GFF1771 at 75.928 kb on - strand, within GFF1771 at 76.110 kb on - strand, within GFF1771 at 76.241 kb on - strand, within GFF1771 at 76.241 kb on - strand, within GFF1771 at 76.251 kb on - strand, within GFF1771 at 76.282 kb on - strand, within GFF1771 at 76.283 kb on + strand, within GFF1771 at 76.404 kb on - strand at 76.404 kb on - strand at 76.891 kb on + strand, within GFF1772 at 77.023 kb on - strand, within GFF1772 at 77.023 kb on - strand, within GFF1772 at 77.169 kb on + strand, within GFF1772 at 77.224 kb on + strand, within GFF1772 at 77.228 kb on - strand, within GFF1772 at 77.323 kb on - strand, within GFF1772 at 77.334 kb on + strand, within GFF1772 at 77.335 kb on - strand, within GFF1772 at 77.385 kb on + strand, within GFF1772 at 77.431 kb on + strand, within GFF1772 at 77.431 kb on + strand, within GFF1772 at 77.431 kb on + strand, within GFF1772 at 77.461 kb on + strand, within GFF1772
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 74,358 - GFF1769 0.85 -0.2 74,358 - GFF1769 0.85 -0.4 74,413 - +0.3 74,480 - -0.2 74,480 - -0.3 74,549 + -0.0 74,556 + GFF1770 0.10 -0.0 74,556 + GFF1770 0.10 +0.2 74,557 - GFF1770 0.10 +0.6 74,601 + GFF1770 0.16 +0.3 74,601 + GFF1770 0.16 +0.4 74,679 + GFF1770 0.27 +0.5 74,680 - GFF1770 0.27 -0.1 74,705 + GFF1770 0.31 +0.2 74,768 + GFF1770 0.39 +1.2 74,768 + GFF1770 0.39 -0.4 74,768 + GFF1770 0.39 -0.4 74,769 - GFF1770 0.40 +0.1 74,770 + GFF1770 0.40 +0.3 74,878 + GFF1770 0.54 -0.0 74,945 + GFF1770 0.64 +0.0 75,113 - GFF1770 0.87 +0.4 75,209 + +0.4 75,458 + GFF1771 0.13 +0.1 75,458 + GFF1771 0.13 +2.2 75,461 + GFF1771 0.13 +0.1 75,461 + GFF1771 0.13 -0.1 75,461 + GFF1771 0.13 +0.7 75,475 - GFF1771 0.15 +0.3 75,484 - GFF1771 0.15 -0.1 75,485 + GFF1771 0.15 +1.9 75,486 - GFF1771 0.15 -0.3 75,558 - GFF1771 0.22 +0.3 75,558 - GFF1771 0.22 -0.4 75,578 + GFF1771 0.23 +0.5 75,579 - GFF1771 0.23 +0.3 75,702 - GFF1771 0.34 +0.1 75,732 + GFF1771 0.36 +2.0 75,732 + GFF1771 0.36 +0.1 75,732 + GFF1771 0.36 -0.5 75,733 - GFF1771 0.36 -0.0 75,733 - GFF1771 0.36 +0.1 75,733 - GFF1771 0.36 +0.4 75,909 - GFF1771 0.51 +0.2 75,927 + GFF1771 0.53 +1.3 75,928 - GFF1771 0.53 -0.6 76,110 - GFF1771 0.68 -0.2 76,241 - GFF1771 0.79 +0.3 76,241 - GFF1771 0.79 +0.2 76,251 - GFF1771 0.80 -0.2 76,282 - GFF1771 0.83 -0.1 76,283 + GFF1771 0.83 +0.1 76,404 - +0.3 76,404 - +1.7 76,891 + GFF1772 0.16 +0.4 77,023 - GFF1772 0.21 -0.9 77,023 - GFF1772 0.21 -0.4 77,169 + GFF1772 0.27 -0.3 77,224 + GFF1772 0.29 -0.1 77,228 - GFF1772 0.29 +0.4 77,323 - GFF1772 0.33 +0.3 77,334 + GFF1772 0.34 +0.3 77,335 - GFF1772 0.34 +0.3 77,385 + GFF1772 0.36 +0.1 77,431 + GFF1772 0.37 +0.1 77,431 + GFF1772 0.37 +0.8 77,431 + GFF1772 0.37 +0.2 77,461 + GFF1772 0.39 +1.4
Or see this region's nucleotide sequence