Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1632 and GFF1633 are separated by 123 nucleotides GFF1633 and GFF1634 are separated by 72 nucleotides
GFF1632 - Phosphogluconate repressor HexR, RpiR family, at 17,905 to 18,774
GFF1632
GFF1633 - Pyruvate kinase (EC 2.7.1.40), at 18,898 to 20,340
GFF1633
GFF1634 - Lipid A biosynthesis (KDO) 2-(lauroyl)-lipid IVA acyltransferase (EC 2.3.1.-), at 20,413 to 21,384
GFF1634
Position (kb) 18
19
20
21 Strain fitness (log2 ratio) -2
-1
0
1 at 17.910 kb on + strand at 18.008 kb on + strand, within GFF1632 at 18.142 kb on + strand, within GFF1632 at 18.143 kb on - strand, within GFF1632 at 18.184 kb on + strand, within GFF1632 at 18.186 kb on + strand, within GFF1632 at 18.187 kb on - strand, within GFF1632 at 18.275 kb on + strand, within GFF1632 at 18.348 kb on + strand, within GFF1632 at 18.415 kb on - strand, within GFF1632 at 18.594 kb on + strand, within GFF1632 at 18.605 kb on - strand, within GFF1632 at 18.606 kb on + strand, within GFF1632 at 18.644 kb on + strand, within GFF1632 at 18.644 kb on + strand, within GFF1632 at 18.645 kb on - strand, within GFF1632 at 18.666 kb on + strand, within GFF1632 at 18.749 kb on + strand at 18.792 kb on + strand at 18.826 kb on - strand at 18.892 kb on + strand at 18.895 kb on + strand at 18.895 kb on + strand at 18.895 kb on + strand at 18.895 kb on + strand at 18.896 kb on - strand at 18.999 kb on - strand at 18.999 kb on - strand at 19.002 kb on - strand at 19.002 kb on - strand at 19.006 kb on - strand at 19.100 kb on + strand, within GFF1633 at 19.384 kb on - strand, within GFF1633 at 19.384 kb on - strand, within GFF1633 at 19.472 kb on - strand, within GFF1633 at 20.323 kb on - strand at 20.339 kb on - strand at 20.369 kb on + strand at 20.498 kb on + strand at 20.625 kb on + strand, within GFF1634 at 20.634 kb on - strand, within GFF1634 at 20.634 kb on - strand, within GFF1634 at 20.708 kb on + strand, within GFF1634 at 20.709 kb on - strand, within GFF1634 at 20.769 kb on - strand, within GFF1634 at 20.874 kb on - strand, within GFF1634 at 20.899 kb on - strand, within GFF1634
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 17,910 + -0.1 18,008 + GFF1632 0.12 -0.9 18,142 + GFF1632 0.27 +0.2 18,143 - GFF1632 0.27 -0.5 18,184 + GFF1632 0.32 -0.3 18,186 + GFF1632 0.32 +0.0 18,187 - GFF1632 0.32 +0.4 18,275 + GFF1632 0.43 +0.9 18,348 + GFF1632 0.51 -0.0 18,415 - GFF1632 0.59 -1.6 18,594 + GFF1632 0.79 +0.7 18,605 - GFF1632 0.80 +0.0 18,606 + GFF1632 0.81 +0.3 18,644 + GFF1632 0.85 -0.4 18,644 + GFF1632 0.85 +0.4 18,645 - GFF1632 0.85 -0.5 18,666 + GFF1632 0.87 -0.4 18,749 + +0.7 18,792 + -0.1 18,826 - -0.8 18,892 + -0.4 18,895 + -0.2 18,895 + -0.5 18,895 + -0.2 18,895 + -1.0 18,896 - -2.0 18,999 - +0.7 18,999 - -0.1 19,002 - -0.7 19,002 - -0.1 19,006 - -0.0 19,100 + GFF1633 0.14 -0.7 19,384 - GFF1633 0.34 -0.8 19,384 - GFF1633 0.34 -0.2 19,472 - GFF1633 0.40 +0.3 20,323 - -0.3 20,339 - +0.1 20,369 + +0.8 20,498 + -1.7 20,625 + GFF1634 0.22 -1.9 20,634 - GFF1634 0.23 -2.3 20,634 - GFF1634 0.23 -0.1 20,708 + GFF1634 0.30 -1.3 20,709 - GFF1634 0.30 -1.3 20,769 - GFF1634 0.37 -0.1 20,874 - GFF1634 0.47 +0.9 20,899 - GFF1634 0.50 -2.7
Or see this region's nucleotide sequence