Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1565 and GFF1566 are separated by 21 nucleotides GFF1566 and GFF1567 are separated by 14 nucleotides
GFF1565 - L-2-hydroxyglutarate oxidase (EC 1.1.3.15), at 27,026 to 28,294
GFF1565
GFF1566 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 28,316 to 29,764
GFF1566
GFF1567 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 29,779 to 31,062
GFF1567
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1 at 27.415 kb on + strand, within GFF1565 at 27.524 kb on + strand, within GFF1565 at 27.525 kb on - strand, within GFF1565 at 27.546 kb on + strand, within GFF1565 at 27.736 kb on + strand, within GFF1565 at 27.750 kb on - strand, within GFF1565 at 28.173 kb on + strand at 28.241 kb on - strand at 28.241 kb on - strand at 28.285 kb on + strand at 28.285 kb on + strand at 28.316 kb on - strand at 28.324 kb on + strand at 28.325 kb on - strand at 28.333 kb on + strand at 28.334 kb on - strand at 28.538 kb on - strand, within GFF1566 at 28.690 kb on + strand, within GFF1566 at 28.691 kb on - strand, within GFF1566 at 28.694 kb on + strand, within GFF1566 at 28.695 kb on - strand, within GFF1566 at 28.695 kb on - strand, within GFF1566 at 28.933 kb on + strand, within GFF1566 at 28.934 kb on - strand, within GFF1566 at 29.194 kb on + strand, within GFF1566 at 29.194 kb on + strand, within GFF1566 at 29.195 kb on - strand, within GFF1566 at 29.195 kb on - strand, within GFF1566 at 29.201 kb on + strand, within GFF1566 at 29.565 kb on - strand, within GFF1566 at 30.026 kb on - strand, within GFF1567 at 30.208 kb on + strand, within GFF1567 at 30.209 kb on - strand, within GFF1567 at 30.209 kb on - strand, within GFF1567 at 30.277 kb on + strand, within GFF1567 at 30.461 kb on + strand, within GFF1567 at 30.481 kb on - strand, within GFF1567 at 30.564 kb on + strand, within GFF1567 at 30.565 kb on - strand, within GFF1567 at 30.753 kb on + strand, within GFF1567
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 27,415 + GFF1565 0.31 +0.4 27,524 + GFF1565 0.39 +0.7 27,525 - GFF1565 0.39 +1.5 27,546 + GFF1565 0.41 -0.5 27,736 + GFF1565 0.56 -0.8 27,750 - GFF1565 0.57 +0.3 28,173 + -0.2 28,241 - +0.4 28,241 - +0.4 28,285 + +0.5 28,285 + -0.1 28,316 - +0.6 28,324 + +0.6 28,325 - +0.3 28,333 + -0.1 28,334 - -0.2 28,538 - GFF1566 0.15 +0.3 28,690 + GFF1566 0.26 +0.7 28,691 - GFF1566 0.26 -0.1 28,694 + GFF1566 0.26 +0.0 28,695 - GFF1566 0.26 +0.6 28,695 - GFF1566 0.26 +0.4 28,933 + GFF1566 0.43 +0.9 28,934 - GFF1566 0.43 -0.2 29,194 + GFF1566 0.61 +0.7 29,194 + GFF1566 0.61 -0.2 29,195 - GFF1566 0.61 +0.0 29,195 - GFF1566 0.61 +0.2 29,201 + GFF1566 0.61 +0.0 29,565 - GFF1566 0.86 -0.8 30,026 - GFF1567 0.19 +0.5 30,208 + GFF1567 0.33 +0.6 30,209 - GFF1567 0.33 -0.2 30,209 - GFF1567 0.33 -0.2 30,277 + GFF1567 0.39 +0.4 30,461 + GFF1567 0.53 -0.2 30,481 - GFF1567 0.55 -0.1 30,564 + GFF1567 0.61 -0.3 30,565 - GFF1567 0.61 +0.2 30,753 + GFF1567 0.76 -0.0
Or see this region's nucleotide sequence