Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1523 and GFF1524 are separated by 43 nucleotides GFF1524 and GFF1525 are separated by 3 nucleotides GFF1525 and GFF1526 are separated by 30 nucleotides GFF1526 and GFF1527 are separated by 14 nucleotides
GFF1523 - Galactitol-1-phosphate 5-dehydrogenase (EC 1.1.1.251), at 27,200 to 28,243
GFF1523
GFF1524 - PTS system, galactitol-specific IIC component (EC 2.7.1.69), at 28,287 to 29,660
GFF1524
GFF1525 - PTS system, galactitol-specific IIB component (EC 2.7.1.69), at 29,664 to 29,948
GFF1525
GFF1526 - PTS system, galactitol-specific IIA component (EC 2.7.1.69), at 29,979 to 30,443
GFF1526
GFF1527 - Tagatose-6-phosphate kinase GatZ (EC 2.7.1.144), at 30,458 to 31,729
GFF1527
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1
2 at 27.375 kb on + strand, within GFF1523 at 27.431 kb on - strand, within GFF1523 at 27.459 kb on - strand, within GFF1523 at 27.459 kb on - strand, within GFF1523 at 27.620 kb on - strand, within GFF1523 at 27.749 kb on - strand, within GFF1523 at 27.753 kb on - strand, within GFF1523 at 27.795 kb on - strand, within GFF1523 at 27.795 kb on - strand, within GFF1523 at 27.917 kb on + strand, within GFF1523 at 27.917 kb on + strand, within GFF1523 at 27.928 kb on - strand, within GFF1523 at 28.029 kb on - strand, within GFF1523 at 28.073 kb on + strand, within GFF1523 at 28.079 kb on - strand, within GFF1523 at 28.082 kb on - strand, within GFF1523 at 28.087 kb on - strand, within GFF1523 at 28.089 kb on + strand, within GFF1523 at 28.090 kb on - strand, within GFF1523 at 28.381 kb on + strand at 28.381 kb on + strand at 28.386 kb on - strand at 28.386 kb on - strand at 28.386 kb on - strand at 28.425 kb on - strand, within GFF1524 at 28.689 kb on + strand, within GFF1524 at 28.692 kb on + strand, within GFF1524 at 28.692 kb on + strand, within GFF1524 at 28.692 kb on + strand, within GFF1524 at 28.725 kb on + strand, within GFF1524 at 28.815 kb on + strand, within GFF1524 at 28.815 kb on + strand, within GFF1524 at 28.860 kb on - strand, within GFF1524 at 28.868 kb on + strand, within GFF1524 at 28.883 kb on + strand, within GFF1524 at 29.042 kb on + strand, within GFF1524 at 29.076 kb on - strand, within GFF1524 at 29.149 kb on + strand, within GFF1524 at 29.285 kb on + strand, within GFF1524 at 29.329 kb on - strand, within GFF1524 at 29.534 kb on + strand at 29.549 kb on + strand at 29.638 kb on + strand at 29.784 kb on - strand, within GFF1525 at 29.784 kb on - strand, within GFF1525 at 29.790 kb on - strand, within GFF1525 at 29.808 kb on - strand, within GFF1525 at 29.810 kb on - strand, within GFF1525 at 29.841 kb on + strand, within GFF1525 at 29.856 kb on - strand, within GFF1525 at 29.974 kb on + strand at 30.115 kb on - strand, within GFF1526 at 30.223 kb on + strand, within GFF1526 at 30.224 kb on - strand, within GFF1526 at 30.341 kb on + strand, within GFF1526 at 30.432 kb on + strand at 30.433 kb on - strand at 30.596 kb on + strand, within GFF1527 at 30.597 kb on - strand, within GFF1527
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 27,375 + GFF1523 0.17 +0.1 27,431 - GFF1523 0.22 +0.1 27,459 - GFF1523 0.25 -0.1 27,459 - GFF1523 0.25 +0.1 27,620 - GFF1523 0.40 +0.4 27,749 - GFF1523 0.53 -0.2 27,753 - GFF1523 0.53 -0.5 27,795 - GFF1523 0.57 +0.6 27,795 - GFF1523 0.57 +0.5 27,917 + GFF1523 0.69 +0.4 27,917 + GFF1523 0.69 +1.0 27,928 - GFF1523 0.70 +0.6 28,029 - GFF1523 0.79 -0.2 28,073 + GFF1523 0.84 -0.4 28,079 - GFF1523 0.84 -0.5 28,082 - GFF1523 0.84 -0.5 28,087 - GFF1523 0.85 -0.1 28,089 + GFF1523 0.85 +0.8 28,090 - GFF1523 0.85 +0.2 28,381 + +1.4 28,381 + +0.8 28,386 - +0.6 28,386 - +0.5 28,386 - +0.2 28,425 - GFF1524 0.10 +0.6 28,689 + GFF1524 0.29 +0.2 28,692 + GFF1524 0.29 +0.1 28,692 + GFF1524 0.29 +0.1 28,692 + GFF1524 0.29 -0.2 28,725 + GFF1524 0.32 +0.3 28,815 + GFF1524 0.38 +0.4 28,815 + GFF1524 0.38 +0.0 28,860 - GFF1524 0.42 +1.6 28,868 + GFF1524 0.42 +0.1 28,883 + GFF1524 0.43 +0.8 29,042 + GFF1524 0.55 +0.3 29,076 - GFF1524 0.57 +0.1 29,149 + GFF1524 0.63 -0.1 29,285 + GFF1524 0.73 +0.5 29,329 - GFF1524 0.76 +0.1 29,534 + -0.3 29,549 + -0.5 29,638 + +0.4 29,784 - GFF1525 0.42 +0.5 29,784 - GFF1525 0.42 +1.1 29,790 - GFF1525 0.44 +1.3 29,808 - GFF1525 0.51 +0.3 29,810 - GFF1525 0.51 +0.1 29,841 + GFF1525 0.62 -1.1 29,856 - GFF1525 0.67 +0.0 29,974 + -0.0 30,115 - GFF1526 0.29 -0.2 30,223 + GFF1526 0.52 -0.2 30,224 - GFF1526 0.53 +0.0 30,341 + GFF1526 0.78 -0.0 30,432 + +0.1 30,433 - +1.9 30,596 + GFF1527 0.11 -0.9 30,597 - GFF1527 0.11 -0.3
Or see this region's nucleotide sequence