Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1453 and GFF1454 are separated by 111 nucleotides GFF1454 and GFF1455 overlap by 4 nucleotides GFF1455 and GFF1456 overlap by 4 nucleotides
GFF1453 - Cytochrome c552 precursor (EC 1.7.2.2), at 116,235 to 117,671
GFF1453
GFF1454 - Cytochrome c-type protein NrfB precursor, at 117,783 to 118,349
GFF1454
GFF1455 - NrfC protein, at 118,346 to 119,017
GFF1455
GFF1456 - NrfD protein, at 119,014 to 119,970
GFF1456
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-1
0
1 at 116.881 kb on - strand, within GFF1453 at 116.909 kb on + strand, within GFF1453 at 116.955 kb on + strand, within GFF1453 at 116.966 kb on + strand, within GFF1453 at 117.137 kb on + strand, within GFF1453 at 117.277 kb on + strand, within GFF1453 at 117.278 kb on - strand, within GFF1453 at 117.278 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.474 kb on - strand, within GFF1453 at 117.575 kb on + strand at 117.576 kb on - strand at 117.737 kb on - strand at 117.766 kb on + strand at 117.767 kb on - strand at 117.771 kb on - strand at 117.771 kb on - strand at 117.782 kb on + strand at 117.783 kb on - strand at 117.803 kb on - strand at 117.998 kb on + strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.095 kb on - strand, within GFF1454 at 118.100 kb on - strand, within GFF1454 at 118.121 kb on + strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.441 kb on + strand, within GFF1455 at 118.559 kb on - strand, within GFF1455 at 118.769 kb on - strand, within GFF1455 at 118.795 kb on + strand, within GFF1455 at 118.940 kb on + strand, within GFF1455 at 118.994 kb on - strand at 118.997 kb on + strand at 118.998 kb on - strand at 119.075 kb on - strand at 119.164 kb on - strand, within GFF1456 at 119.164 kb on - strand, within GFF1456 at 119.275 kb on + strand, within GFF1456 at 119.323 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.331 kb on - strand, within GFF1456
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 116,881 - GFF1453 0.45 +0.0 116,909 + GFF1453 0.47 -0.0 116,955 + GFF1453 0.50 -0.0 116,966 + GFF1453 0.51 +0.3 117,137 + GFF1453 0.63 -0.2 117,277 + GFF1453 0.73 -0.0 117,278 - GFF1453 0.73 +0.2 117,278 - GFF1453 0.73 -0.5 117,408 - GFF1453 0.82 +0.4 117,408 - GFF1453 0.82 -0.4 117,474 - GFF1453 0.86 +0.0 117,575 + +1.1 117,576 - +0.7 117,737 - -0.2 117,766 + +0.6 117,767 - -0.2 117,771 - +0.9 117,771 - -0.2 117,782 + -0.4 117,783 - -0.1 117,803 - -0.3 117,998 + GFF1454 0.38 -0.5 118,093 - GFF1454 0.55 -0.5 118,093 - GFF1454 0.55 -0.1 118,095 - GFF1454 0.55 -0.2 118,100 - GFF1454 0.56 -0.9 118,121 + GFF1454 0.60 +0.1 118,122 - GFF1454 0.60 +0.3 118,122 - GFF1454 0.60 +0.3 118,441 + GFF1455 0.14 +0.1 118,559 - GFF1455 0.32 +0.7 118,769 - GFF1455 0.63 -0.4 118,795 + GFF1455 0.67 +0.3 118,940 + GFF1455 0.88 -0.3 118,994 - +0.3 118,997 + +0.6 118,998 - +1.3 119,075 - +0.2 119,164 - GFF1456 0.16 +0.4 119,164 - GFF1456 0.16 -0.3 119,275 + GFF1456 0.27 -0.7 119,323 + GFF1456 0.32 -1.2 119,330 + GFF1456 0.33 +0.0 119,330 + GFF1456 0.33 +0.0 119,331 - GFF1456 0.33 +0.0
Or see this region's nucleotide sequence