Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1382 and GFF1383 overlap by 1 nucleotides GFF1383 and GFF1384 overlap by 1 nucleotides GFF1384 and GFF1385 are separated by 96 nucleotides GFF1385 and GFF1386 overlap by 41 nucleotides GFF1386 and GFF1387 are separated by 51 nucleotides
GFF1382 - Putative inner membrane protein, at 29,500 to 29,709
GFF1382
GFF1383 - FIG00639790: hypothetical protein, at 29,709 to 30,662
GFF1383
GFF1384 - Phage tail length tape-measure protein, at 30,662 to 33,016
GFF1384
GFF1385 - hypothetical protein, at 33,113 to 33,241
GFF1385
GFF1386 - FIG00639134: hypothetical protein, at 33,201 to 33,518
GFF1386
GFF1387 - Putative phage tail core protein, at 33,570 to 34,094
GFF1387
Position (kb) 30
31
32
33
34 Strain fitness (log2 ratio) -1
0
1 at 29.703 kb on - strand at 29.724 kb on - strand at 30.162 kb on - strand, within GFF1383 at 30.584 kb on - strand at 30.600 kb on - strand at 30.663 kb on + strand at 30.664 kb on - strand at 30.842 kb on - strand at 30.842 kb on - strand at 30.843 kb on + strand at 30.843 kb on + strand at 31.162 kb on + strand, within GFF1384 at 31.217 kb on + strand, within GFF1384 at 31.363 kb on - strand, within GFF1384 at 31.367 kb on + strand, within GFF1384 at 31.820 kb on - strand, within GFF1384 at 31.925 kb on + strand, within GFF1384 at 32.427 kb on + strand, within GFF1384 at 32.428 kb on - strand, within GFF1384 at 32.428 kb on - strand, within GFF1384 at 32.434 kb on + strand, within GFF1384 at 32.559 kb on + strand, within GFF1384 at 33.001 kb on + strand at 33.019 kb on + strand at 33.173 kb on - strand, within GFF1385 at 33.314 kb on - strand, within GFF1386 at 33.447 kb on + strand, within GFF1386 at 33.448 kb on - strand, within GFF1386 at 33.485 kb on + strand, within GFF1386 at 33.522 kb on - strand at 33.582 kb on - strand at 33.813 kb on - strand, within GFF1387 at 33.872 kb on + strand, within GFF1387 at 33.984 kb on - strand, within GFF1387 at 34.008 kb on - strand, within GFF1387
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 29,703 - -0.3 29,724 - -0.1 30,162 - GFF1383 0.47 +1.5 30,584 - +0.8 30,600 - -0.4 30,663 + -1.0 30,664 - +1.0 30,842 - -0.1 30,842 - +0.0 30,843 + -0.4 30,843 + -0.2 31,162 + GFF1384 0.21 +0.0 31,217 + GFF1384 0.24 +0.0 31,363 - GFF1384 0.30 +0.2 31,367 + GFF1384 0.30 +0.2 31,820 - GFF1384 0.49 +0.1 31,925 + GFF1384 0.54 +0.1 32,427 + GFF1384 0.75 +0.2 32,428 - GFF1384 0.75 -0.1 32,428 - GFF1384 0.75 -0.2 32,434 + GFF1384 0.75 +0.3 32,559 + GFF1384 0.81 -1.6 33,001 + -0.1 33,019 + -0.4 33,173 - GFF1385 0.47 +0.3 33,314 - GFF1386 0.36 -0.3 33,447 + GFF1386 0.77 -0.6 33,448 - GFF1386 0.78 -0.0 33,485 + GFF1386 0.89 +0.2 33,522 - +0.7 33,582 - -0.2 33,813 - GFF1387 0.46 +1.0 33,872 + GFF1387 0.58 -0.2 33,984 - GFF1387 0.79 +0.2 34,008 - GFF1387 0.83 +0.3
Or see this region's nucleotide sequence