Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1363

Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1361 and GFF1362 are separated by 46 nucleotidesGFF1362 and GFF1363 overlap by 4 nucleotidesGFF1363 and GFF1364 are separated by 97 nucleotides GFF1361 - Nucleoprotein/polynucleotide-associated enzyme, at 15,021 to 15,560 GFF1361 GFF1362 - Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70), at 15,607 to 16,476 GFF1362 GFF1363 - hypothetical protein, at 16,473 to 16,745 GFF1363 GFF1364 - Sodium-dependent transporter, at 16,843 to 17,784 GFF1364 Position (kb) 16 17Strain fitness (log2 ratio) -1 0 1 2at 15.559 kb on - strandat 15.559 kb on - strandat 15.583 kb on - strandat 15.606 kb on + strandat 15.640 kb on + strandat 15.640 kb on + strandat 15.640 kb on + strandat 15.642 kb on + strandat 15.806 kb on + strand, within GFF1362at 15.806 kb on + strand, within GFF1362at 15.948 kb on - strand, within GFF1362at 16.107 kb on + strand, within GFF1362at 16.180 kb on - strand, within GFF1362at 16.537 kb on + strand, within GFF1363at 16.601 kb on + strand, within GFF1363at 16.610 kb on - strand, within GFF1363at 17.107 kb on - strand, within GFF1364at 17.151 kb on + strand, within GFF1364at 17.152 kb on - strand, within GFF1364at 17.455 kb on + strand, within GFF1364at 17.456 kb on - strand, within GFF1364at 17.563 kb on + strand, within GFF1364at 17.661 kb on + strand, within GFF1364at 17.662 kb on - strand, within GFF1364at 17.720 kb on + strandat 17.723 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI
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15,559 - +0.4
15,559 - +2.0
15,583 - +1.8
15,606 + +0.3
15,640 + +1.2
15,640 + -0.2
15,640 + +1.0
15,642 + +0.3
15,806 + GFF1362 0.23 +0.1
15,806 + GFF1362 0.23 +0.4
15,948 - GFF1362 0.39 +0.2
16,107 + GFF1362 0.57 -0.2
16,180 - GFF1362 0.66 -0.0
16,537 + GFF1363 0.23 -0.9
16,601 + GFF1363 0.47 -0.3
16,610 - GFF1363 0.50 -0.8
17,107 - GFF1364 0.28 -0.2
17,151 + GFF1364 0.33 +0.4
17,152 - GFF1364 0.33 -0.6
17,455 + GFF1364 0.65 -0.4
17,456 - GFF1364 0.65 +0.3
17,563 + GFF1364 0.76 -0.3
17,661 + GFF1364 0.87 +0.5
17,662 - GFF1364 0.87 +0.0
17,720 + +0.7
17,723 + -0.1

Or see this region's nucleotide sequence