Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1069 and GFF1070 are separated by 40 nucleotides GFF1070 and GFF1071 are separated by 17 nucleotides
GFF1069 - Putative major fimbrial subunit, at 251,163 to 252,248
GFF1069
GFF1070 - putative fimbrial subunit, at 252,289 to 252,846
GFF1070
GFF1071 - type 1 fimbriae anchoring protein FimD, at 252,864 to 255,293
GFF1071
Position (kb) 252
253 Strain fitness (log2 ratio) -1
0
1
2 at 251.403 kb on + strand, within GFF1069 at 251.463 kb on - strand, within GFF1069 at 251.510 kb on + strand, within GFF1069 at 251.536 kb on - strand, within GFF1069 at 251.664 kb on - strand, within GFF1069 at 251.834 kb on + strand, within GFF1069 at 251.835 kb on - strand, within GFF1069 at 251.850 kb on - strand, within GFF1069 at 251.854 kb on + strand, within GFF1069 at 251.858 kb on + strand, within GFF1069 at 251.859 kb on - strand, within GFF1069 at 251.884 kb on + strand, within GFF1069 at 251.991 kb on - strand, within GFF1069 at 251.991 kb on - strand, within GFF1069 at 252.016 kb on + strand, within GFF1069 at 252.017 kb on - strand, within GFF1069 at 252.018 kb on + strand, within GFF1069 at 252.019 kb on - strand, within GFF1069 at 252.019 kb on - strand, within GFF1069 at 252.032 kb on + strand, within GFF1069 at 252.032 kb on + strand, within GFF1069 at 252.044 kb on + strand, within GFF1069 at 252.170 kb on + strand at 252.193 kb on + strand at 252.194 kb on - strand at 252.198 kb on - strand at 252.209 kb on - strand at 252.236 kb on - strand at 252.236 kb on - strand at 252.357 kb on + strand, within GFF1070 at 252.359 kb on + strand, within GFF1070 at 252.359 kb on + strand, within GFF1070 at 252.359 kb on + strand, within GFF1070 at 252.359 kb on + strand, within GFF1070 at 252.360 kb on - strand, within GFF1070 at 252.420 kb on - strand, within GFF1070 at 252.515 kb on + strand, within GFF1070 at 252.550 kb on + strand, within GFF1070 at 252.550 kb on + strand, within GFF1070 at 252.666 kb on - strand, within GFF1070 at 252.716 kb on + strand, within GFF1070 at 252.721 kb on + strand, within GFF1070 at 252.722 kb on - strand, within GFF1070 at 252.761 kb on + strand, within GFF1070 at 252.761 kb on + strand, within GFF1070 at 252.841 kb on - strand at 252.851 kb on + strand at 252.882 kb on + strand at 252.918 kb on + strand at 252.920 kb on + strand at 252.920 kb on + strand at 253.140 kb on - strand, within GFF1071 at 253.140 kb on - strand, within GFF1071 at 253.191 kb on - strand, within GFF1071 at 253.401 kb on + strand, within GFF1071 at 253.401 kb on + strand, within GFF1071 at 253.407 kb on + strand, within GFF1071 at 253.443 kb on - strand, within GFF1071 at 253.454 kb on - strand, within GFF1071 at 253.458 kb on - strand, within GFF1071 at 253.458 kb on - strand, within GFF1071 at 253.480 kb on + strand, within GFF1071 at 253.480 kb on + strand, within GFF1071 at 253.481 kb on - strand, within GFF1071 at 253.481 kb on - strand, within GFF1071 at 253.481 kb on - strand, within GFF1071 at 253.601 kb on + strand, within GFF1071 at 253.671 kb on + strand, within GFF1071 at 253.672 kb on - strand, within GFF1071 at 253.763 kb on - strand, within GFF1071 at 253.772 kb on + strand, within GFF1071 at 253.773 kb on - strand, within GFF1071 at 253.773 kb on - strand, within GFF1071 at 253.785 kb on + strand, within GFF1071 at 253.785 kb on + strand, within GFF1071 at 253.786 kb on - strand, within GFF1071 at 253.786 kb on - strand, within GFF1071 at 253.786 kb on - strand, within GFF1071 at 253.819 kb on - strand, within GFF1071 at 253.819 kb on - strand, within GFF1071 at 253.830 kb on - strand, within GFF1071
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 251,403 + GFF1069 0.22 -1.1 251,463 - GFF1069 0.28 -0.4 251,510 + GFF1069 0.32 +0.5 251,536 - GFF1069 0.34 +0.1 251,664 - GFF1069 0.46 +1.6 251,834 + GFF1069 0.62 -0.1 251,835 - GFF1069 0.62 -1.1 251,850 - GFF1069 0.63 -0.1 251,854 + GFF1069 0.64 -0.2 251,858 + GFF1069 0.64 +0.0 251,859 - GFF1069 0.64 +0.6 251,884 + GFF1069 0.66 +0.4 251,991 - GFF1069 0.76 +0.4 251,991 - GFF1069 0.76 +0.2 252,016 + GFF1069 0.79 +0.2 252,017 - GFF1069 0.79 +0.9 252,018 + GFF1069 0.79 +0.5 252,019 - GFF1069 0.79 +0.5 252,019 - GFF1069 0.79 +0.4 252,032 + GFF1069 0.80 -0.1 252,032 + GFF1069 0.80 +0.2 252,044 + GFF1069 0.81 +0.3 252,170 + +0.1 252,193 + +0.3 252,194 - -0.3 252,198 - +0.8 252,209 - +0.1 252,236 - -0.4 252,236 - +0.3 252,357 + GFF1070 0.12 +0.4 252,359 + GFF1070 0.13 +1.3 252,359 + GFF1070 0.13 -0.0 252,359 + GFF1070 0.13 +1.5 252,359 + GFF1070 0.13 +0.8 252,360 - GFF1070 0.13 +0.2 252,420 - GFF1070 0.23 +0.3 252,515 + GFF1070 0.41 -0.1 252,550 + GFF1070 0.47 +0.1 252,550 + GFF1070 0.47 +0.1 252,666 - GFF1070 0.68 +0.2 252,716 + GFF1070 0.77 -0.3 252,721 + GFF1070 0.77 -0.4 252,722 - GFF1070 0.78 +0.2 252,761 + GFF1070 0.85 +0.3 252,761 + GFF1070 0.85 +0.0 252,841 - +1.9 252,851 + +0.2 252,882 + +0.4 252,918 + +0.3 252,920 + -0.1 252,920 + -0.3 253,140 - GFF1071 0.11 -0.0 253,140 - GFF1071 0.11 -0.0 253,191 - GFF1071 0.13 -0.1 253,401 + GFF1071 0.22 +0.3 253,401 + GFF1071 0.22 +0.4 253,407 + GFF1071 0.22 +0.2 253,443 - GFF1071 0.24 +0.9 253,454 - GFF1071 0.24 -0.5 253,458 - GFF1071 0.24 -0.5 253,458 - GFF1071 0.24 -0.2 253,480 + GFF1071 0.25 +0.7 253,480 + GFF1071 0.25 -0.0 253,481 - GFF1071 0.25 +0.2 253,481 - GFF1071 0.25 -0.1 253,481 - GFF1071 0.25 +0.5 253,601 + GFF1071 0.30 -0.2 253,671 + GFF1071 0.33 +1.5 253,672 - GFF1071 0.33 +0.0 253,763 - GFF1071 0.37 -0.6 253,772 + GFF1071 0.37 +0.1 253,773 - GFF1071 0.37 -0.2 253,773 - GFF1071 0.37 +0.5 253,785 + GFF1071 0.38 -0.0 253,785 + GFF1071 0.38 +0.1 253,786 - GFF1071 0.38 +0.5 253,786 - GFF1071 0.38 -0.2 253,786 - GFF1071 0.38 -0.3 253,819 - GFF1071 0.39 -0.3 253,819 - GFF1071 0.39 -0.3 253,830 - GFF1071 0.40 -0.2
Or see this region's nucleotide sequence