Experiment: LB_plus_SM_buffer with Ffm_phage 18.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1067 and GFF1068 are separated by 57 nucleotides GFF1068 and GFF1069 are separated by 57 nucleotides
GFF1067 - Two-component response regulator CreC, at 248,274 to 249,698
GFF1067
GFF1068 - Inner membrane protein CreD, at 249,756 to 251,105
GFF1068
GFF1069 - Putative major fimbrial subunit, at 251,163 to 252,248
GFF1069
Position (kb) 249
250
251
252 Strain fitness (log2 ratio) -1
0
1
2 at 249.054 kb on + strand, within GFF1067 at 249.055 kb on - strand, within GFF1067 at 249.055 kb on - strand, within GFF1067 at 249.055 kb on - strand, within GFF1067 at 249.163 kb on + strand, within GFF1067 at 249.271 kb on + strand, within GFF1067 at 249.272 kb on - strand, within GFF1067 at 249.276 kb on - strand, within GFF1067 at 249.446 kb on - strand, within GFF1067 at 249.446 kb on - strand, within GFF1067 at 249.544 kb on - strand, within GFF1067 at 249.696 kb on + strand at 249.697 kb on - strand at 249.726 kb on + strand at 249.756 kb on - strand at 249.878 kb on - strand at 249.991 kb on - strand, within GFF1068 at 250.002 kb on + strand, within GFF1068 at 250.003 kb on - strand, within GFF1068 at 250.003 kb on - strand, within GFF1068 at 250.003 kb on - strand, within GFF1068 at 250.069 kb on + strand, within GFF1068 at 250.069 kb on + strand, within GFF1068 at 250.073 kb on + strand, within GFF1068 at 250.074 kb on - strand, within GFF1068 at 250.182 kb on - strand, within GFF1068 at 250.764 kb on + strand, within GFF1068 at 250.764 kb on + strand, within GFF1068 at 250.794 kb on - strand, within GFF1068 at 250.855 kb on - strand, within GFF1068 at 250.955 kb on + strand, within GFF1068 at 250.956 kb on - strand, within GFF1068 at 250.956 kb on - strand, within GFF1068 at 251.089 kb on + strand at 251.165 kb on - strand at 251.168 kb on + strand at 251.168 kb on + strand at 251.170 kb on + strand at 251.170 kb on + strand at 251.170 kb on + strand at 251.171 kb on - strand at 251.171 kb on - strand at 251.171 kb on - strand at 251.171 kb on - strand at 251.182 kb on - strand at 251.267 kb on + strand at 251.403 kb on + strand, within GFF1069 at 251.463 kb on - strand, within GFF1069 at 251.510 kb on + strand, within GFF1069 at 251.536 kb on - strand, within GFF1069 at 251.664 kb on - strand, within GFF1069 at 251.834 kb on + strand, within GFF1069 at 251.835 kb on - strand, within GFF1069 at 251.850 kb on - strand, within GFF1069 at 251.854 kb on + strand, within GFF1069 at 251.858 kb on + strand, within GFF1069 at 251.859 kb on - strand, within GFF1069 at 251.884 kb on + strand, within GFF1069 at 251.991 kb on - strand, within GFF1069 at 251.991 kb on - strand, within GFF1069 at 252.016 kb on + strand, within GFF1069 at 252.017 kb on - strand, within GFF1069 at 252.018 kb on + strand, within GFF1069 at 252.019 kb on - strand, within GFF1069 at 252.019 kb on - strand, within GFF1069 at 252.032 kb on + strand, within GFF1069 at 252.032 kb on + strand, within GFF1069 at 252.044 kb on + strand, within GFF1069
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 18.75 MOI remove 249,054 + GFF1067 0.55 +0.6 249,055 - GFF1067 0.55 +0.6 249,055 - GFF1067 0.55 +1.2 249,055 - GFF1067 0.55 -0.1 249,163 + GFF1067 0.62 +0.0 249,271 + GFF1067 0.70 +0.6 249,272 - GFF1067 0.70 -0.2 249,276 - GFF1067 0.70 -0.3 249,446 - GFF1067 0.82 +0.5 249,446 - GFF1067 0.82 +0.2 249,544 - GFF1067 0.89 -0.4 249,696 + +0.5 249,697 - +0.1 249,726 + +0.5 249,756 - +0.2 249,878 - +0.0 249,991 - GFF1068 0.17 +0.6 250,002 + GFF1068 0.18 +0.4 250,003 - GFF1068 0.18 +0.5 250,003 - GFF1068 0.18 -0.0 250,003 - GFF1068 0.18 +2.1 250,069 + GFF1068 0.23 +0.3 250,069 + GFF1068 0.23 +0.5 250,073 + GFF1068 0.23 -0.3 250,074 - GFF1068 0.24 +0.5 250,182 - GFF1068 0.32 -0.2 250,764 + GFF1068 0.75 +0.4 250,764 + GFF1068 0.75 -0.2 250,794 - GFF1068 0.77 -0.1 250,855 - GFF1068 0.81 +0.3 250,955 + GFF1068 0.89 -0.2 250,956 - GFF1068 0.89 +0.6 250,956 - GFF1068 0.89 +1.1 251,089 + +1.2 251,165 - +0.5 251,168 + +0.5 251,168 + -0.7 251,170 + -0.0 251,170 + -0.2 251,170 + -0.3 251,171 - +0.2 251,171 - +1.5 251,171 - -0.2 251,171 - -0.2 251,182 - -0.1 251,267 + +0.7 251,403 + GFF1069 0.22 -1.1 251,463 - GFF1069 0.28 -0.4 251,510 + GFF1069 0.32 +0.5 251,536 - GFF1069 0.34 +0.1 251,664 - GFF1069 0.46 +1.6 251,834 + GFF1069 0.62 -0.1 251,835 - GFF1069 0.62 -1.1 251,850 - GFF1069 0.63 -0.1 251,854 + GFF1069 0.64 -0.2 251,858 + GFF1069 0.64 +0.0 251,859 - GFF1069 0.64 +0.6 251,884 + GFF1069 0.66 +0.4 251,991 - GFF1069 0.76 +0.4 251,991 - GFF1069 0.76 +0.2 252,016 + GFF1069 0.79 +0.2 252,017 - GFF1069 0.79 +0.9 252,018 + GFF1069 0.79 +0.5 252,019 - GFF1069 0.79 +0.5 252,019 - GFF1069 0.79 +0.4 252,032 + GFF1069 0.80 -0.1 252,032 + GFF1069 0.80 +0.2 252,044 + GFF1069 0.81 +0.3
Or see this region's nucleotide sequence