Strain Fitness in Escherichia coli BL21 around ECD_00004

Experiment: Mixed culture, Halobacteriovorax-1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00002 and ECD_00003 are separated by 1 nucleotidesECD_00003 and ECD_00004 are separated by 0 nucleotidesECD_00004 and ECD_00005 are separated by 212 nucleotidesECD_00005 and ECD_00006 are separated by 152 nucleotides ECD_00002: ECD_00002 - Bifunctional aspartokinase/homoserine dehydrogenase 1, at 336 to 2,798 _00002 ECD_00003: ECD_00003 - homoserine kinase, at 2,800 to 3,732 _00003 ECD_00004: ECD_00004 - L-threonine synthase, at 3,733 to 5,019 _00004 ECD_00005: ECD_00005 - DUF2502 family putative periplasmic protein, at 5,232 to 5,528 _00005 ECD_00006: ECD_00006 - peroxide resistance protein, lowers intracellular iron, at 5,681 to 6,457 _00006 Position (kb) 3 4 5 6Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2.767 kb on + strandat 2.767 kb on + strandat 2.768 kb on - strandat 2.768 kb on - strandat 2.771 kb on + strandat 2.788 kb on + strandat 2.789 kb on - strandat 2.805 kb on + strandat 2.903 kb on + strand, within ECD_00003at 2.903 kb on + strand, within ECD_00003at 2.903 kb on + strand, within ECD_00003at 2.904 kb on - strand, within ECD_00003at 3.154 kb on - strand, within ECD_00003at 3.218 kb on - strand, within ECD_00003at 3.249 kb on + strand, within ECD_00003at 3.250 kb on - strand, within ECD_00003at 3.339 kb on + strand, within ECD_00003at 3.443 kb on - strand, within ECD_00003at 3.443 kb on - strand, within ECD_00003at 3.651 kb on + strandat 3.659 kb on - strandat 3.659 kb on - strandat 3.659 kb on - strandat 3.659 kb on - strandat 3.687 kb on + strandat 3.687 kb on + strandat 3.688 kb on - strandat 3.719 kb on + strandat 3.730 kb on + strandat 3.743 kb on - strandat 3.743 kb on - strandat 4.245 kb on + strand, within ECD_00004at 4.245 kb on + strand, within ECD_00004at 4.245 kb on + strand, within ECD_00004at 4.246 kb on - strand, within ECD_00004at 4.246 kb on - strand, within ECD_00004at 4.409 kb on - strand, within ECD_00004at 4.409 kb on - strand, within ECD_00004at 4.775 kb on - strand, within ECD_00004at 4.805 kb on - strand, within ECD_00004at 4.840 kb on + strand, within ECD_00004at 4.950 kb on - strandat 4.965 kb on + strandat 4.966 kb on - strandat 4.996 kb on - strandat 5.025 kb on - strandat 5.217 kb on + strandat 5.218 kb on - strandat 5.226 kb on + strandat 5.231 kb on + strandat 5.250 kb on - strandat 5.309 kb on + strand, within ECD_00005at 5.351 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.471 kb on + strand, within ECD_00005at 5.472 kb on - strand, within ECD_00005at 5.615 kb on - strandat 5.803 kb on + strand, within ECD_00006at 5.804 kb on - strand, within ECD_00006at 5.804 kb on - strand, within ECD_00006at 5.834 kb on - strand, within ECD_00006at 5.839 kb on - strand, within ECD_00006at 5.855 kb on - strand, within ECD_00006at 5.954 kb on - strand, within ECD_00006at 6.010 kb on + strand, within ECD_00006at 6.011 kb on - strand, within ECD_00006at 6.011 kb on - strand, within ECD_00006at 6.011 kb on - strand, within ECD_00006

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Halobacteriovorax-1
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2,767 + -1.8
2,767 + -0.5
2,768 - -2.1
2,768 - -2.1
2,771 + -1.3
2,788 + -0.6
2,789 - -1.7
2,805 + -1.7
2,903 + ECD_00003 0.11 -2.5
2,903 + ECD_00003 0.11 -1.0
2,903 + ECD_00003 0.11 -2.5
2,904 - ECD_00003 0.11 -1.6
3,154 - ECD_00003 0.38 -2.1
3,218 - ECD_00003 0.45 -0.6
3,249 + ECD_00003 0.48 -1.7
3,250 - ECD_00003 0.48 -1.8
3,339 + ECD_00003 0.58 -2.2
3,443 - ECD_00003 0.69 -1.7
3,443 - ECD_00003 0.69 -1.4
3,651 + -0.9
3,659 - -1.8
3,659 - -1.7
3,659 - -0.9
3,659 - -1.8
3,687 + -2.6
3,687 + -2.3
3,688 - -1.9
3,719 + -0.1
3,730 + +0.4
3,743 - -3.6
3,743 - -2.6
4,245 + ECD_00004 0.40 -4.2
4,245 + ECD_00004 0.40 -2.8
4,245 + ECD_00004 0.40 -1.9
4,246 - ECD_00004 0.40 -1.7
4,246 - ECD_00004 0.40 -2.9
4,409 - ECD_00004 0.53 -2.7
4,409 - ECD_00004 0.53 -0.9
4,775 - ECD_00004 0.81 -3.2
4,805 - ECD_00004 0.83 -1.8
4,840 + ECD_00004 0.86 -0.6
4,950 - -2.1
4,965 + -2.1
4,966 - -2.1
4,996 - -0.7
5,025 - -0.4
5,217 + +0.1
5,218 - -0.5
5,226 + +0.8
5,231 + -0.2
5,250 - -1.3
5,309 + ECD_00005 0.26 -0.8
5,351 + ECD_00005 0.40 +0.1
5,412 + ECD_00005 0.61 +0.2
5,412 + ECD_00005 0.61 +1.0
5,412 + ECD_00005 0.61 -0.4
5,412 + ECD_00005 0.61 +0.3
5,471 + ECD_00005 0.80 +0.8
5,472 - ECD_00005 0.81 -0.3
5,615 - -0.3
5,803 + ECD_00006 0.16 +0.6
5,804 - ECD_00006 0.16 -0.1
5,804 - ECD_00006 0.16 +0.7
5,834 - ECD_00006 0.20 +1.0
5,839 - ECD_00006 0.20 -0.8
5,855 - ECD_00006 0.22 +0.0
5,954 - ECD_00006 0.35 -1.1
6,010 + ECD_00006 0.42 -0.3
6,011 - ECD_00006 0.42 -0.0
6,011 - ECD_00006 0.42 -0.1
6,011 - ECD_00006 0.42 -0.5

Or see this region's nucleotide sequence