Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2289 and B158DRAFT_2290 overlap by 4 nucleotides B158DRAFT_2290 and B158DRAFT_2291 overlap by 13 nucleotides B158DRAFT_2291 and B158DRAFT_2292 are separated by 12 nucleotides
B158DRAFT_2289: B158DRAFT_2289 - imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 454,537 to 455,133
_2289
B158DRAFT_2290: B158DRAFT_2290 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 455,130 to 455,738
_2290
B158DRAFT_2291: B158DRAFT_2291 - imidazoleglycerol phosphate synthase, cyclase subunit, at 455,726 to 456,529
_2291
B158DRAFT_2292: B158DRAFT_2292 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16)/phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 456,542 to 457,780
_2292
Position (kb)
455
456
457 Strain fitness (log2 ratio)
-1
0
1 at 454.797 kb on + strand, within B158DRAFT_2289 at 454.797 kb on + strand, within B158DRAFT_2289 at 454.805 kb on - strand, within B158DRAFT_2289 at 454.858 kb on + strand, within B158DRAFT_2289 at 454.863 kb on + strand, within B158DRAFT_2289 at 454.866 kb on - strand, within B158DRAFT_2289 at 454.874 kb on + strand, within B158DRAFT_2289 at 454.876 kb on + strand, within B158DRAFT_2289 at 454.968 kb on + strand, within B158DRAFT_2289 at 454.969 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.044 kb on + strand, within B158DRAFT_2289 at 455.069 kb on + strand, within B158DRAFT_2289 at 455.077 kb on - strand at 455.093 kb on + strand at 455.122 kb on - strand at 455.129 kb on - strand at 455.137 kb on + strand at 455.137 kb on + strand at 455.224 kb on + strand, within B158DRAFT_2290 at 455.230 kb on + strand, within B158DRAFT_2290 at 455.246 kb on - strand, within B158DRAFT_2290 at 455.276 kb on + strand, within B158DRAFT_2290 at 455.279 kb on - strand, within B158DRAFT_2290 at 455.349 kb on - strand, within B158DRAFT_2290 at 455.391 kb on + strand, within B158DRAFT_2290 at 455.398 kb on + strand, within B158DRAFT_2290 at 455.409 kb on + strand, within B158DRAFT_2290 at 455.412 kb on - strand, within B158DRAFT_2290 at 455.412 kb on - strand, within B158DRAFT_2290 at 455.420 kb on - strand, within B158DRAFT_2290 at 455.591 kb on + strand, within B158DRAFT_2290 at 455.606 kb on + strand, within B158DRAFT_2290 at 455.614 kb on - strand, within B158DRAFT_2290 at 455.727 kb on + strand at 455.792 kb on + strand at 455.795 kb on - strand at 455.799 kb on + strand at 455.828 kb on + strand, within B158DRAFT_2291 at 455.836 kb on + strand, within B158DRAFT_2291 at 455.836 kb on + strand, within B158DRAFT_2291 at 455.836 kb on - strand, within B158DRAFT_2291 at 455.836 kb on - strand, within B158DRAFT_2291 at 455.844 kb on - strand, within B158DRAFT_2291 at 455.844 kb on - strand, within B158DRAFT_2291 at 455.844 kb on - strand, within B158DRAFT_2291 at 455.845 kb on - strand, within B158DRAFT_2291 at 455.927 kb on - strand, within B158DRAFT_2291 at 455.949 kb on + strand, within B158DRAFT_2291 at 455.957 kb on - strand, within B158DRAFT_2291 at 456.064 kb on - strand, within B158DRAFT_2291 at 456.126 kb on + strand, within B158DRAFT_2291 at 456.257 kb on + strand, within B158DRAFT_2291 at 456.257 kb on + strand, within B158DRAFT_2291 at 456.263 kb on + strand, within B158DRAFT_2291 at 456.314 kb on + strand, within B158DRAFT_2291 at 456.322 kb on + strand, within B158DRAFT_2291 at 456.493 kb on + strand at 456.493 kb on + strand at 456.503 kb on - strand at 456.503 kb on - strand at 456.555 kb on + strand at 456.571 kb on + strand at 456.595 kb on + strand at 456.603 kb on - strand at 456.700 kb on + strand, within B158DRAFT_2292 at 456.703 kb on - strand, within B158DRAFT_2292 at 456.708 kb on - strand, within B158DRAFT_2292 at 456.708 kb on - strand, within B158DRAFT_2292 at 456.709 kb on - strand, within B158DRAFT_2292 at 456.796 kb on - strand, within B158DRAFT_2292 at 456.810 kb on - strand, within B158DRAFT_2292 at 456.926 kb on - strand, within B158DRAFT_2292 at 456.939 kb on - strand, within B158DRAFT_2292 at 456.995 kb on + strand, within B158DRAFT_2292 at 456.997 kb on - strand, within B158DRAFT_2292 at 457.055 kb on - strand, within B158DRAFT_2292 at 457.055 kb on - strand, within B158DRAFT_2292 at 457.106 kb on + strand, within B158DRAFT_2292 at 457.114 kb on - strand, within B158DRAFT_2292 at 457.159 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.255 kb on - strand, within B158DRAFT_2292 at 457.376 kb on - strand, within B158DRAFT_2292 at 457.410 kb on - strand, within B158DRAFT_2292 at 457.467 kb on - strand, within B158DRAFT_2292
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 454,797 + B158DRAFT_2289 0.44 -1.0 454,797 + B158DRAFT_2289 0.44 -0.4 454,805 - B158DRAFT_2289 0.45 -0.3 454,858 + B158DRAFT_2289 0.54 -0.4 454,863 + B158DRAFT_2289 0.55 -0.2 454,866 - B158DRAFT_2289 0.55 +0.2 454,874 + B158DRAFT_2289 0.56 -0.1 454,876 + B158DRAFT_2289 0.57 -0.3 454,968 + B158DRAFT_2289 0.72 +1.0 454,969 + B158DRAFT_2289 0.72 -1.0 455,007 + B158DRAFT_2289 0.79 -0.6 455,007 + B158DRAFT_2289 0.79 +0.0 455,007 + B158DRAFT_2289 0.79 -0.1 455,007 + B158DRAFT_2289 0.79 +0.1 455,015 - B158DRAFT_2289 0.80 +0.0 455,015 - B158DRAFT_2289 0.80 -0.2 455,015 - B158DRAFT_2289 0.80 +0.1 455,015 - B158DRAFT_2289 0.80 -0.5 455,044 + B158DRAFT_2289 0.85 +0.5 455,069 + B158DRAFT_2289 0.89 -0.6 455,077 - -1.4 455,093 + -0.5 455,122 - -0.4 455,129 - +0.0 455,137 + -0.1 455,137 + +0.1 455,224 + B158DRAFT_2290 0.15 +0.5 455,230 + B158DRAFT_2290 0.16 +0.8 455,246 - B158DRAFT_2290 0.19 -0.0 455,276 + B158DRAFT_2290 0.24 +0.3 455,279 - B158DRAFT_2290 0.24 +0.2 455,349 - B158DRAFT_2290 0.36 +0.7 455,391 + B158DRAFT_2290 0.43 -0.7 455,398 + B158DRAFT_2290 0.44 +0.5 455,409 + B158DRAFT_2290 0.46 +1.0 455,412 - B158DRAFT_2290 0.46 -0.2 455,412 - B158DRAFT_2290 0.46 +0.0 455,420 - B158DRAFT_2290 0.48 -0.4 455,591 + B158DRAFT_2290 0.76 -0.2 455,606 + B158DRAFT_2290 0.78 +0.1 455,614 - B158DRAFT_2290 0.79 -0.0 455,727 + -0.2 455,792 + +1.7 455,795 - -0.3 455,799 + -0.6 455,828 + B158DRAFT_2291 0.13 +0.9 455,836 + B158DRAFT_2291 0.14 +0.0 455,836 + B158DRAFT_2291 0.14 -1.0 455,836 - B158DRAFT_2291 0.14 -0.1 455,836 - B158DRAFT_2291 0.14 +0.3 455,844 - B158DRAFT_2291 0.15 +1.0 455,844 - B158DRAFT_2291 0.15 -0.0 455,844 - B158DRAFT_2291 0.15 +0.2 455,845 - B158DRAFT_2291 0.15 -1.1 455,927 - B158DRAFT_2291 0.25 -0.1 455,949 + B158DRAFT_2291 0.28 -0.3 455,957 - B158DRAFT_2291 0.29 -0.2 456,064 - B158DRAFT_2291 0.42 +0.4 456,126 + B158DRAFT_2291 0.50 -0.3 456,257 + B158DRAFT_2291 0.66 +0.3 456,257 + B158DRAFT_2291 0.66 +1.1 456,263 + B158DRAFT_2291 0.67 +0.8 456,314 + B158DRAFT_2291 0.73 +0.6 456,322 + B158DRAFT_2291 0.74 -0.4 456,493 + +0.6 456,493 + -0.4 456,503 - -1.4 456,503 - +0.1 456,555 + -0.0 456,571 + -0.1 456,595 + -1.1 456,603 - +0.1 456,700 + B158DRAFT_2292 0.13 +0.2 456,703 - B158DRAFT_2292 0.13 -0.2 456,708 - B158DRAFT_2292 0.13 -0.7 456,708 - B158DRAFT_2292 0.13 -0.4 456,709 - B158DRAFT_2292 0.13 -0.6 456,796 - B158DRAFT_2292 0.21 +0.7 456,810 - B158DRAFT_2292 0.22 -0.3 456,926 - B158DRAFT_2292 0.31 +0.0 456,939 - B158DRAFT_2292 0.32 -0.5 456,995 + B158DRAFT_2292 0.37 +0.6 456,997 - B158DRAFT_2292 0.37 -1.0 457,055 - B158DRAFT_2292 0.41 +0.8 457,055 - B158DRAFT_2292 0.41 +0.6 457,106 + B158DRAFT_2292 0.46 +0.3 457,114 - B158DRAFT_2292 0.46 +0.6 457,159 - B158DRAFT_2292 0.50 +0.7 457,207 - B158DRAFT_2292 0.54 +0.3 457,207 - B158DRAFT_2292 0.54 -0.0 457,207 - B158DRAFT_2292 0.54 +0.6 457,207 - B158DRAFT_2292 0.54 +0.0 457,207 - B158DRAFT_2292 0.54 +0.3 457,255 - B158DRAFT_2292 0.58 -0.2 457,376 - B158DRAFT_2292 0.67 -0.0 457,410 - B158DRAFT_2292 0.70 -0.3 457,467 - B158DRAFT_2292 0.75 -0.2
Or see this region's nucleotide sequence