Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2150 and B158DRAFT_2152 are separated by 416 nucleotides B158DRAFT_2152 and B158DRAFT_2153 are separated by 212 nucleotides B158DRAFT_2153 and B158DRAFT_2154 overlap by 1 nucleotides
B158DRAFT_2150: B158DRAFT_2150 - Glucose/sorbosone dehydrogenases, at 288,586 to 289,671
_2150
B158DRAFT_2152: B158DRAFT_2152 - Predicted metalloendopeptidase, at 290,088 to 292,181
_2152
B158DRAFT_2153: B158DRAFT_2153 - Predicted thioesterase, at 292,394 to 292,846
_2153
B158DRAFT_2154: B158DRAFT_2154 - tRNA s(2)C-32 sulfurtransferase (EC 2.8.1.4), at 292,846 to 293,769
_2154
Position (kb)
290
291
292
293 Strain fitness (log2 ratio)
-2
-1
0
1 at 289.142 kb on - strand, within B158DRAFT_2150 at 289.142 kb on - strand, within B158DRAFT_2150 at 289.156 kb on - strand, within B158DRAFT_2150 at 289.218 kb on + strand, within B158DRAFT_2150 at 289.218 kb on + strand, within B158DRAFT_2150 at 289.286 kb on + strand, within B158DRAFT_2150 at 289.292 kb on - strand, within B158DRAFT_2150 at 289.336 kb on - strand, within B158DRAFT_2150 at 289.336 kb on - strand, within B158DRAFT_2150 at 289.338 kb on + strand, within B158DRAFT_2150 at 289.399 kb on + strand, within B158DRAFT_2150 at 289.442 kb on + strand, within B158DRAFT_2150 at 289.515 kb on + strand, within B158DRAFT_2150 at 289.525 kb on + strand, within B158DRAFT_2150 at 289.533 kb on - strand, within B158DRAFT_2150 at 289.564 kb on + strand at 289.564 kb on + strand at 289.572 kb on - strand at 289.572 kb on - strand at 289.572 kb on - strand at 289.572 kb on - strand at 289.638 kb on + strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.651 kb on - strand at 290.196 kb on + strand at 290.209 kb on - strand at 290.305 kb on + strand, within B158DRAFT_2152 at 290.377 kb on - strand, within B158DRAFT_2152 at 290.416 kb on + strand, within B158DRAFT_2152 at 290.431 kb on + strand, within B158DRAFT_2152 at 290.441 kb on - strand, within B158DRAFT_2152 at 290.605 kb on + strand, within B158DRAFT_2152 at 290.689 kb on + strand, within B158DRAFT_2152 at 290.689 kb on + strand, within B158DRAFT_2152 at 290.689 kb on + strand, within B158DRAFT_2152 at 290.719 kb on + strand, within B158DRAFT_2152 at 290.719 kb on + strand, within B158DRAFT_2152 at 290.727 kb on - strand, within B158DRAFT_2152 at 290.727 kb on - strand, within B158DRAFT_2152 at 290.802 kb on - strand, within B158DRAFT_2152 at 290.839 kb on + strand, within B158DRAFT_2152 at 290.847 kb on - strand, within B158DRAFT_2152 at 290.934 kb on - strand, within B158DRAFT_2152 at 290.939 kb on + strand, within B158DRAFT_2152 at 290.960 kb on - strand, within B158DRAFT_2152 at 290.960 kb on - strand, within B158DRAFT_2152 at 291.038 kb on - strand, within B158DRAFT_2152 at 291.101 kb on + strand, within B158DRAFT_2152 at 291.237 kb on - strand, within B158DRAFT_2152 at 291.269 kb on + strand, within B158DRAFT_2152 at 291.331 kb on + strand, within B158DRAFT_2152 at 291.339 kb on - strand, within B158DRAFT_2152 at 291.578 kb on + strand, within B158DRAFT_2152 at 291.586 kb on - strand, within B158DRAFT_2152 at 291.586 kb on - strand, within B158DRAFT_2152 at 291.634 kb on + strand, within B158DRAFT_2152 at 291.641 kb on + strand, within B158DRAFT_2152 at 291.641 kb on + strand, within B158DRAFT_2152 at 291.656 kb on + strand, within B158DRAFT_2152 at 291.666 kb on + strand, within B158DRAFT_2152 at 291.698 kb on + strand, within B158DRAFT_2152 at 291.698 kb on + strand, within B158DRAFT_2152 at 291.754 kb on + strand, within B158DRAFT_2152 at 291.754 kb on + strand, within B158DRAFT_2152 at 291.762 kb on - strand, within B158DRAFT_2152 at 291.826 kb on - strand, within B158DRAFT_2152 at 291.882 kb on + strand, within B158DRAFT_2152 at 291.882 kb on + strand, within B158DRAFT_2152 at 291.882 kb on + strand, within B158DRAFT_2152 at 291.887 kb on + strand, within B158DRAFT_2152 at 291.887 kb on + strand, within B158DRAFT_2152 at 291.983 kb on + strand at 292.240 kb on + strand at 292.273 kb on + strand at 292.281 kb on - strand at 292.302 kb on + strand at 292.310 kb on - strand at 292.363 kb on - strand at 292.367 kb on + strand at 292.367 kb on - strand at 292.372 kb on + strand at 292.375 kb on - strand at 292.380 kb on - strand at 292.380 kb on - strand at 292.380 kb on - strand at 292.392 kb on + strand at 292.392 kb on + strand at 292.474 kb on - strand, within B158DRAFT_2153 at 292.510 kb on - strand, within B158DRAFT_2153 at 292.522 kb on - strand, within B158DRAFT_2153 at 292.522 kb on - strand, within B158DRAFT_2153 at 292.596 kb on - strand, within B158DRAFT_2153 at 292.626 kb on + strand, within B158DRAFT_2153 at 292.626 kb on + strand, within B158DRAFT_2153 at 292.645 kb on + strand, within B158DRAFT_2153 at 292.645 kb on + strand, within B158DRAFT_2153 at 292.645 kb on + strand, within B158DRAFT_2153 at 292.651 kb on + strand, within B158DRAFT_2153 at 292.653 kb on - strand, within B158DRAFT_2153 at 292.653 kb on - strand, within B158DRAFT_2153 at 292.653 kb on - strand, within B158DRAFT_2153 at 292.659 kb on - strand, within B158DRAFT_2153 at 292.692 kb on - strand, within B158DRAFT_2153 at 292.778 kb on + strand, within B158DRAFT_2153 at 292.795 kb on - strand, within B158DRAFT_2153 at 292.844 kb on + strand at 292.934 kb on + strand at 292.947 kb on - strand, within B158DRAFT_2154 at 292.959 kb on + strand, within B158DRAFT_2154 at 292.959 kb on + strand, within B158DRAFT_2154 at 292.967 kb on - strand, within B158DRAFT_2154 at 293.025 kb on - strand, within B158DRAFT_2154 at 293.063 kb on + strand, within B158DRAFT_2154 at 293.063 kb on + strand, within B158DRAFT_2154 at 293.181 kb on - strand, within B158DRAFT_2154
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 289,142 - B158DRAFT_2150 0.51 -0.6 289,142 - B158DRAFT_2150 0.51 -0.0 289,156 - B158DRAFT_2150 0.52 +0.4 289,218 + B158DRAFT_2150 0.58 +0.7 289,218 + B158DRAFT_2150 0.58 +0.5 289,286 + B158DRAFT_2150 0.64 -0.3 289,292 - B158DRAFT_2150 0.65 -0.7 289,336 - B158DRAFT_2150 0.69 -0.3 289,336 - B158DRAFT_2150 0.69 +0.2 289,338 + B158DRAFT_2150 0.69 +0.2 289,399 + B158DRAFT_2150 0.75 +0.5 289,442 + B158DRAFT_2150 0.79 -0.4 289,515 + B158DRAFT_2150 0.86 +0.5 289,525 + B158DRAFT_2150 0.86 +0.1 289,533 - B158DRAFT_2150 0.87 +0.1 289,564 + -0.8 289,564 + +0.2 289,572 - +0.1 289,572 - -0.6 289,572 - -0.1 289,572 - +0.1 289,638 + +0.2 289,646 - +0.2 289,646 - -0.1 289,646 - +0.9 289,646 - +0.9 289,646 - +0.8 289,651 - +0.5 290,196 + -0.1 290,209 - +0.0 290,305 + B158DRAFT_2152 0.10 -0.9 290,377 - B158DRAFT_2152 0.14 -0.1 290,416 + B158DRAFT_2152 0.16 +0.9 290,431 + B158DRAFT_2152 0.16 -0.3 290,441 - B158DRAFT_2152 0.17 +0.3 290,605 + B158DRAFT_2152 0.25 +0.2 290,689 + B158DRAFT_2152 0.29 -0.2 290,689 + B158DRAFT_2152 0.29 +0.1 290,689 + B158DRAFT_2152 0.29 -0.1 290,719 + B158DRAFT_2152 0.30 -0.8 290,719 + B158DRAFT_2152 0.30 -1.5 290,727 - B158DRAFT_2152 0.31 +0.1 290,727 - B158DRAFT_2152 0.31 -0.5 290,802 - B158DRAFT_2152 0.34 -0.1 290,839 + B158DRAFT_2152 0.36 -0.9 290,847 - B158DRAFT_2152 0.36 -0.6 290,934 - B158DRAFT_2152 0.40 -0.2 290,939 + B158DRAFT_2152 0.41 +0.1 290,960 - B158DRAFT_2152 0.42 +0.6 290,960 - B158DRAFT_2152 0.42 -0.3 291,038 - B158DRAFT_2152 0.45 -0.3 291,101 + B158DRAFT_2152 0.48 -0.8 291,237 - B158DRAFT_2152 0.55 +0.0 291,269 + B158DRAFT_2152 0.56 +0.5 291,331 + B158DRAFT_2152 0.59 +0.1 291,339 - B158DRAFT_2152 0.60 +0.3 291,578 + B158DRAFT_2152 0.71 -0.1 291,586 - B158DRAFT_2152 0.72 -0.5 291,586 - B158DRAFT_2152 0.72 +0.0 291,634 + B158DRAFT_2152 0.74 -0.3 291,641 + B158DRAFT_2152 0.74 -1.0 291,641 + B158DRAFT_2152 0.74 -0.7 291,656 + B158DRAFT_2152 0.75 +0.1 291,666 + B158DRAFT_2152 0.75 -0.7 291,698 + B158DRAFT_2152 0.77 -0.8 291,698 + B158DRAFT_2152 0.77 +0.2 291,754 + B158DRAFT_2152 0.80 -0.7 291,754 + B158DRAFT_2152 0.80 -0.4 291,762 - B158DRAFT_2152 0.80 +0.1 291,826 - B158DRAFT_2152 0.83 -0.2 291,882 + B158DRAFT_2152 0.86 +0.1 291,882 + B158DRAFT_2152 0.86 -0.1 291,882 + B158DRAFT_2152 0.86 +0.0 291,887 + B158DRAFT_2152 0.86 +0.2 291,887 + B158DRAFT_2152 0.86 -0.2 291,983 + -0.1 292,240 + -0.1 292,273 + -0.1 292,281 - +0.2 292,302 + +0.3 292,310 - +0.0 292,363 - -0.0 292,367 + +0.1 292,367 - -0.1 292,372 + -0.0 292,375 - -0.1 292,380 - -1.0 292,380 - -0.4 292,380 - -0.5 292,392 + -0.9 292,392 + -0.2 292,474 - B158DRAFT_2153 0.18 -2.2 292,510 - B158DRAFT_2153 0.26 +0.9 292,522 - B158DRAFT_2153 0.28 +0.1 292,522 - B158DRAFT_2153 0.28 +0.2 292,596 - B158DRAFT_2153 0.45 -0.8 292,626 + B158DRAFT_2153 0.51 +0.1 292,626 + B158DRAFT_2153 0.51 -0.1 292,645 + B158DRAFT_2153 0.55 +0.4 292,645 + B158DRAFT_2153 0.55 -0.3 292,645 + B158DRAFT_2153 0.55 -0.1 292,651 + B158DRAFT_2153 0.57 -0.2 292,653 - B158DRAFT_2153 0.57 +0.6 292,653 - B158DRAFT_2153 0.57 -0.3 292,653 - B158DRAFT_2153 0.57 +0.5 292,659 - B158DRAFT_2153 0.58 +0.0 292,692 - B158DRAFT_2153 0.66 +0.0 292,778 + B158DRAFT_2153 0.85 +0.1 292,795 - B158DRAFT_2153 0.89 -0.3 292,844 + -0.8 292,934 + -1.0 292,947 - B158DRAFT_2154 0.11 -0.2 292,959 + B158DRAFT_2154 0.12 +1.3 292,959 + B158DRAFT_2154 0.12 -0.0 292,967 - B158DRAFT_2154 0.13 -0.2 293,025 - B158DRAFT_2154 0.19 +0.1 293,063 + B158DRAFT_2154 0.23 +0.3 293,063 + B158DRAFT_2154 0.23 -0.5 293,181 - B158DRAFT_2154 0.36 +0.1
Or see this region's nucleotide sequence