Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2145 and B158DRAFT_2146 are separated by 137 nucleotides B158DRAFT_2146 and B158DRAFT_2147 are separated by 287 nucleotides B158DRAFT_2147 and B158DRAFT_2148 are separated by 256 nucleotides
B158DRAFT_2145: B158DRAFT_2145 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form, at 281,196 to 282,854
_2145
B158DRAFT_2146: B158DRAFT_2146 - dihydrolipoamide dehydrogenase, at 282,992 to 284,410
_2146
B158DRAFT_2147: B158DRAFT_2147 - hypothetical protein, at 284,698 to 284,814
_2147
B158DRAFT_2148: B158DRAFT_2148 - Predicted permeases, at 285,071 to 286,009
_2148
Position (kb)
282
283
284
285 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 282.030 kb on - strand, within B158DRAFT_2145 at 282.057 kb on + strand, within B158DRAFT_2145 at 282.065 kb on - strand, within B158DRAFT_2145 at 282.083 kb on + strand, within B158DRAFT_2145 at 282.320 kb on + strand, within B158DRAFT_2145 at 282.320 kb on + strand, within B158DRAFT_2145 at 282.389 kb on + strand, within B158DRAFT_2145 at 282.396 kb on - strand, within B158DRAFT_2145 at 282.483 kb on - strand, within B158DRAFT_2145 at 282.516 kb on + strand, within B158DRAFT_2145 at 282.588 kb on + strand, within B158DRAFT_2145 at 282.609 kb on - strand, within B158DRAFT_2145 at 282.626 kb on - strand, within B158DRAFT_2145 at 282.640 kb on + strand, within B158DRAFT_2145 at 282.640 kb on + strand, within B158DRAFT_2145 at 282.648 kb on - strand, within B158DRAFT_2145 at 282.661 kb on + strand, within B158DRAFT_2145 at 282.698 kb on + strand at 282.735 kb on + strand at 282.752 kb on + strand at 282.760 kb on - strand at 282.841 kb on - strand at 284.383 kb on + strand at 284.494 kb on + strand at 284.494 kb on + strand at 284.494 kb on + strand at 284.494 kb on + strand at 284.502 kb on - strand at 284.580 kb on + strand at 284.681 kb on - strand at 284.695 kb on + strand at 284.696 kb on + strand at 284.848 kb on - strand at 284.852 kb on + strand at 284.862 kb on - strand at 284.978 kb on - strand at 284.978 kb on - strand at 284.988 kb on + strand at 284.996 kb on - strand at 285.044 kb on - strand at 285.068 kb on + strand at 285.068 kb on + strand at 285.068 kb on + strand at 285.068 kb on + strand at 285.076 kb on - strand at 285.076 kb on - strand at 285.076 kb on - strand at 285.076 kb on - strand at 285.076 kb on - strand at 285.106 kb on + strand at 285.142 kb on - strand at 285.176 kb on + strand, within B158DRAFT_2148 at 285.189 kb on + strand, within B158DRAFT_2148 at 285.346 kb on + strand, within B158DRAFT_2148 at 285.346 kb on + strand, within B158DRAFT_2148 at 285.368 kb on + strand, within B158DRAFT_2148 at 285.376 kb on - strand, within B158DRAFT_2148 at 285.378 kb on + strand, within B158DRAFT_2148 at 285.390 kb on + strand, within B158DRAFT_2148 at 285.393 kb on - strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.395 kb on + strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148 at 285.403 kb on - strand, within B158DRAFT_2148
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 282,030 - B158DRAFT_2145 0.50 +2.5 282,057 + B158DRAFT_2145 0.52 +1.5 282,065 - B158DRAFT_2145 0.52 +0.2 282,083 + B158DRAFT_2145 0.53 +2.2 282,320 + B158DRAFT_2145 0.68 +3.0 282,320 + B158DRAFT_2145 0.68 -0.1 282,389 + B158DRAFT_2145 0.72 +0.8 282,396 - B158DRAFT_2145 0.72 +0.7 282,483 - B158DRAFT_2145 0.78 +3.2 282,516 + B158DRAFT_2145 0.80 +1.5 282,588 + B158DRAFT_2145 0.84 -0.1 282,609 - B158DRAFT_2145 0.85 +0.9 282,626 - B158DRAFT_2145 0.86 +3.1 282,640 + B158DRAFT_2145 0.87 +2.1 282,640 + B158DRAFT_2145 0.87 +2.9 282,648 - B158DRAFT_2145 0.88 +2.5 282,661 + B158DRAFT_2145 0.88 +0.9 282,698 + -0.7 282,735 + +2.6 282,752 + +2.1 282,760 - +1.8 282,841 - +1.5 284,383 + +1.4 284,494 + +0.1 284,494 + +0.1 284,494 + -0.1 284,494 + -0.1 284,502 - -0.3 284,580 + -0.0 284,681 - -0.0 284,695 + +0.2 284,696 + -0.3 284,848 - -0.6 284,852 + -1.0 284,862 - +0.1 284,978 - +0.0 284,978 - -0.1 284,988 + -0.4 284,996 - -0.3 285,044 - -0.6 285,068 + -0.1 285,068 + +0.7 285,068 + -1.2 285,068 + -0.3 285,076 - -0.5 285,076 - -0.6 285,076 - -0.6 285,076 - -0.1 285,076 - +0.1 285,106 + +0.2 285,142 - +0.9 285,176 + B158DRAFT_2148 0.11 -0.2 285,189 + B158DRAFT_2148 0.13 -0.4 285,346 + B158DRAFT_2148 0.29 -0.0 285,346 + B158DRAFT_2148 0.29 +0.7 285,368 + B158DRAFT_2148 0.32 -1.9 285,376 - B158DRAFT_2148 0.32 -0.1 285,378 + B158DRAFT_2148 0.33 +0.2 285,390 + B158DRAFT_2148 0.34 +0.0 285,393 - B158DRAFT_2148 0.34 -0.1 285,395 + B158DRAFT_2148 0.35 -0.2 285,395 + B158DRAFT_2148 0.35 -0.7 285,395 + B158DRAFT_2148 0.35 +0.5 285,395 + B158DRAFT_2148 0.35 -0.6 285,395 + B158DRAFT_2148 0.35 +0.1 285,395 + B158DRAFT_2148 0.35 -0.3 285,395 + B158DRAFT_2148 0.35 -0.3 285,395 + B158DRAFT_2148 0.35 -0.7 285,395 + B158DRAFT_2148 0.35 +0.7 285,395 + B158DRAFT_2148 0.35 -0.6 285,395 + B158DRAFT_2148 0.35 +0.0 285,395 + B158DRAFT_2148 0.35 -1.5 285,395 + B158DRAFT_2148 0.35 -0.4 285,403 - B158DRAFT_2148 0.35 +0.1 285,403 - B158DRAFT_2148 0.35 +0.0 285,403 - B158DRAFT_2148 0.35 -1.1 285,403 - B158DRAFT_2148 0.35 -0.2 285,403 - B158DRAFT_2148 0.35 -1.1 285,403 - B158DRAFT_2148 0.35 -0.5 285,403 - B158DRAFT_2148 0.35 +0.3
Or see this region's nucleotide sequence