Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1905 and B158DRAFT_1906 are separated by 14 nucleotides B158DRAFT_1906 and B158DRAFT_1907 are separated by 118 nucleotides B158DRAFT_1907 and B158DRAFT_1908 are separated by 138 nucleotides
B158DRAFT_1905: B158DRAFT_1905 - cation diffusion facilitator family transporter, at 41,396 to 42,322
_1905
B158DRAFT_1906: B158DRAFT_1906 - Response regulator containing a CheY-like receiver domain and a GGDEF domain, at 42,337 to 42,894
_1906
B158DRAFT_1907: B158DRAFT_1907 - Uncharacterized protein conserved in bacteria, at 43,013 to 43,621
_1907
B158DRAFT_1908: B158DRAFT_1908 - Glycine/serine hydroxymethyltransferase, at 43,760 to 45,013
_1908
Position (kb)
43
44 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 42.057 kb on - strand, within B158DRAFT_1905 at 42.057 kb on - strand, within B158DRAFT_1905 at 42.089 kb on + strand, within B158DRAFT_1905 at 42.097 kb on - strand, within B158DRAFT_1905 at 42.097 kb on - strand, within B158DRAFT_1905 at 42.097 kb on - strand, within B158DRAFT_1905 at 42.097 kb on - strand, within B158DRAFT_1905 at 42.097 kb on - strand, within B158DRAFT_1905 at 42.109 kb on + strand, within B158DRAFT_1905 at 42.109 kb on + strand, within B158DRAFT_1905 at 42.109 kb on + strand, within B158DRAFT_1905 at 42.117 kb on - strand, within B158DRAFT_1905 at 42.135 kb on + strand, within B158DRAFT_1905 at 42.143 kb on - strand, within B158DRAFT_1905 at 42.153 kb on - strand, within B158DRAFT_1905 at 42.181 kb on + strand, within B158DRAFT_1905 at 42.189 kb on - strand, within B158DRAFT_1905 at 42.304 kb on - strand at 42.306 kb on + strand at 42.314 kb on - strand at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.425 kb on + strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.433 kb on - strand, within B158DRAFT_1906 at 42.454 kb on - strand, within B158DRAFT_1906 at 42.470 kb on + strand, within B158DRAFT_1906 at 42.493 kb on + strand, within B158DRAFT_1906 at 42.549 kb on + strand, within B158DRAFT_1906 at 42.550 kb on - strand, within B158DRAFT_1906 at 42.557 kb on - strand, within B158DRAFT_1906 at 42.567 kb on + strand, within B158DRAFT_1906 at 42.588 kb on + strand, within B158DRAFT_1906 at 42.596 kb on - strand, within B158DRAFT_1906 at 42.608 kb on - strand, within B158DRAFT_1906 at 42.639 kb on + strand, within B158DRAFT_1906 at 42.642 kb on - strand, within B158DRAFT_1906 at 42.642 kb on - strand, within B158DRAFT_1906 at 42.730 kb on - strand, within B158DRAFT_1906 at 42.827 kb on + strand, within B158DRAFT_1906 at 42.835 kb on + strand, within B158DRAFT_1906 at 42.835 kb on + strand, within B158DRAFT_1906 at 42.835 kb on + strand, within B158DRAFT_1906 at 42.840 kb on + strand at 42.843 kb on - strand at 42.843 kb on - strand at 42.890 kb on - strand at 43.011 kb on - strand at 43.064 kb on + strand at 43.064 kb on + strand at 43.064 kb on + strand at 43.064 kb on + strand at 43.064 kb on + strand at 43.072 kb on - strand at 43.072 kb on - strand at 43.082 kb on - strand, within B158DRAFT_1907 at 43.113 kb on - strand, within B158DRAFT_1907 at 43.129 kb on + strand, within B158DRAFT_1907 at 43.160 kb on - strand, within B158DRAFT_1907 at 43.170 kb on - strand, within B158DRAFT_1907 at 43.236 kb on - strand, within B158DRAFT_1907 at 43.287 kb on - strand, within B158DRAFT_1907 at 43.324 kb on - strand, within B158DRAFT_1907 at 43.429 kb on - strand, within B158DRAFT_1907 at 43.454 kb on - strand, within B158DRAFT_1907 at 43.458 kb on + strand, within B158DRAFT_1907 at 43.461 kb on - strand, within B158DRAFT_1907 at 43.483 kb on - strand, within B158DRAFT_1907 at 43.507 kb on + strand, within B158DRAFT_1907 at 43.513 kb on + strand, within B158DRAFT_1907 at 43.516 kb on - strand, within B158DRAFT_1907 at 43.539 kb on + strand, within B158DRAFT_1907 at 43.548 kb on - strand, within B158DRAFT_1907 at 43.549 kb on - strand, within B158DRAFT_1907 at 43.554 kb on - strand, within B158DRAFT_1907 at 43.563 kb on + strand at 43.568 kb on + strand at 43.568 kb on + strand at 43.576 kb on - strand at 43.576 kb on - strand at 43.615 kb on + strand at 43.623 kb on - strand at 43.643 kb on - strand at 43.690 kb on + strand at 43.690 kb on + strand at 43.698 kb on - strand at 43.698 kb on - strand at 43.750 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.773 kb on - strand at 43.773 kb on - strand at 43.845 kb on + strand at 43.853 kb on - strand at 43.895 kb on + strand, within B158DRAFT_1908 at 43.902 kb on + strand, within B158DRAFT_1908 at 43.902 kb on + strand, within B158DRAFT_1908 at 43.902 kb on + strand, within B158DRAFT_1908 at 43.907 kb on + strand, within B158DRAFT_1908 at 43.910 kb on - strand, within B158DRAFT_1908 at 43.915 kb on - strand, within B158DRAFT_1908 at 43.930 kb on + strand, within B158DRAFT_1908 at 43.945 kb on + strand, within B158DRAFT_1908 at 43.975 kb on - strand, within B158DRAFT_1908 at 44.022 kb on - strand, within B158DRAFT_1908 at 44.032 kb on - strand, within B158DRAFT_1908 at 44.043 kb on - strand, within B158DRAFT_1908 at 44.055 kb on - strand, within B158DRAFT_1908 at 44.069 kb on + strand, within B158DRAFT_1908 at 44.080 kb on - strand, within B158DRAFT_1908 at 44.080 kb on - strand, within B158DRAFT_1908 at 44.081 kb on + strand, within B158DRAFT_1908 at 44.086 kb on + strand, within B158DRAFT_1908 at 44.086 kb on + strand, within B158DRAFT_1908 at 44.086 kb on + strand, within B158DRAFT_1908 at 44.094 kb on - strand, within B158DRAFT_1908 at 44.094 kb on - strand, within B158DRAFT_1908 at 44.122 kb on + strand, within B158DRAFT_1908 at 44.124 kb on - strand, within B158DRAFT_1908 at 44.130 kb on - strand, within B158DRAFT_1908 at 44.130 kb on - strand, within B158DRAFT_1908 at 44.201 kb on + strand, within B158DRAFT_1908 at 44.215 kb on + strand, within B158DRAFT_1908 at 44.231 kb on + strand, within B158DRAFT_1908 at 44.238 kb on + strand, within B158DRAFT_1908 at 44.239 kb on - strand, within B158DRAFT_1908 at 44.239 kb on - strand, within B158DRAFT_1908 at 44.285 kb on + strand, within B158DRAFT_1908 at 44.292 kb on + strand, within B158DRAFT_1908 at 44.375 kb on + strand, within B158DRAFT_1908 at 44.375 kb on + strand, within B158DRAFT_1908 at 44.383 kb on - strand, within B158DRAFT_1908 at 44.397 kb on + strand, within B158DRAFT_1908 at 44.499 kb on - strand, within B158DRAFT_1908 at 44.517 kb on - strand, within B158DRAFT_1908 at 44.542 kb on - strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.605 kb on + strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908 at 44.613 kb on - strand, within B158DRAFT_1908
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 42,057 - B158DRAFT_1905 0.71 -0.7 42,057 - B158DRAFT_1905 0.71 -0.1 42,089 + B158DRAFT_1905 0.75 -0.1 42,097 - B158DRAFT_1905 0.76 -1.0 42,097 - B158DRAFT_1905 0.76 +0.1 42,097 - B158DRAFT_1905 0.76 +0.3 42,097 - B158DRAFT_1905 0.76 -0.6 42,097 - B158DRAFT_1905 0.76 +0.3 42,109 + B158DRAFT_1905 0.77 +0.1 42,109 + B158DRAFT_1905 0.77 +0.3 42,109 + B158DRAFT_1905 0.77 +0.6 42,117 - B158DRAFT_1905 0.78 +0.2 42,135 + B158DRAFT_1905 0.80 -0.1 42,143 - B158DRAFT_1905 0.81 -0.4 42,153 - B158DRAFT_1905 0.82 -0.2 42,181 + B158DRAFT_1905 0.85 +0.1 42,189 - B158DRAFT_1905 0.86 -0.5 42,304 - -0.9 42,306 + +0.7 42,314 - +0.2 42,425 + B158DRAFT_1906 0.16 -0.1 42,425 + B158DRAFT_1906 0.16 +0.2 42,425 + B158DRAFT_1906 0.16 +0.2 42,425 + B158DRAFT_1906 0.16 +0.2 42,425 + B158DRAFT_1906 0.16 -0.8 42,425 + B158DRAFT_1906 0.16 -0.1 42,425 + B158DRAFT_1906 0.16 +0.1 42,425 + B158DRAFT_1906 0.16 +0.3 42,425 + B158DRAFT_1906 0.16 +0.3 42,425 + B158DRAFT_1906 0.16 -0.4 42,425 + B158DRAFT_1906 0.16 -0.1 42,425 + B158DRAFT_1906 0.16 +0.6 42,433 - B158DRAFT_1906 0.17 +0.3 42,433 - B158DRAFT_1906 0.17 +0.4 42,433 - B158DRAFT_1906 0.17 +0.1 42,433 - B158DRAFT_1906 0.17 -0.1 42,433 - B158DRAFT_1906 0.17 +0.1 42,433 - B158DRAFT_1906 0.17 -0.7 42,433 - B158DRAFT_1906 0.17 -0.2 42,433 - B158DRAFT_1906 0.17 -0.4 42,454 - B158DRAFT_1906 0.21 +0.3 42,470 + B158DRAFT_1906 0.24 -0.2 42,493 + B158DRAFT_1906 0.28 -0.3 42,549 + B158DRAFT_1906 0.38 -0.1 42,550 - B158DRAFT_1906 0.38 -0.2 42,557 - B158DRAFT_1906 0.39 -1.0 42,567 + B158DRAFT_1906 0.41 -0.9 42,588 + B158DRAFT_1906 0.45 -0.1 42,596 - B158DRAFT_1906 0.46 +0.2 42,608 - B158DRAFT_1906 0.49 +0.3 42,639 + B158DRAFT_1906 0.54 +0.0 42,642 - B158DRAFT_1906 0.55 +0.7 42,642 - B158DRAFT_1906 0.55 +0.6 42,730 - B158DRAFT_1906 0.70 -0.4 42,827 + B158DRAFT_1906 0.88 +0.2 42,835 + B158DRAFT_1906 0.89 -0.3 42,835 + B158DRAFT_1906 0.89 +0.2 42,835 + B158DRAFT_1906 0.89 +0.4 42,840 + -1.6 42,843 - -0.2 42,843 - -0.2 42,890 - +0.3 43,011 - -0.8 43,064 + -0.1 43,064 + -0.7 43,064 + -0.5 43,064 + +0.4 43,064 + +0.1 43,072 - +0.3 43,072 - -0.5 43,082 - B158DRAFT_1907 0.11 +0.1 43,113 - B158DRAFT_1907 0.16 -0.4 43,129 + B158DRAFT_1907 0.19 -0.4 43,160 - B158DRAFT_1907 0.24 -0.3 43,170 - B158DRAFT_1907 0.26 -0.1 43,236 - B158DRAFT_1907 0.37 -0.0 43,287 - B158DRAFT_1907 0.45 -0.7 43,324 - B158DRAFT_1907 0.51 -0.3 43,429 - B158DRAFT_1907 0.68 -0.2 43,454 - B158DRAFT_1907 0.72 -0.3 43,458 + B158DRAFT_1907 0.73 +0.6 43,461 - B158DRAFT_1907 0.74 -0.8 43,483 - B158DRAFT_1907 0.77 -0.1 43,507 + B158DRAFT_1907 0.81 +0.5 43,513 + B158DRAFT_1907 0.82 +0.1 43,516 - B158DRAFT_1907 0.83 -0.2 43,539 + B158DRAFT_1907 0.86 -0.2 43,548 - B158DRAFT_1907 0.88 -0.1 43,549 - B158DRAFT_1907 0.88 +0.0 43,554 - B158DRAFT_1907 0.89 +0.1 43,563 + +0.2 43,568 + -0.5 43,568 + +1.5 43,576 - +0.1 43,576 - -0.9 43,615 + +1.0 43,623 - -0.2 43,643 - +2.1 43,690 + -0.1 43,690 + -0.0 43,698 - +0.5 43,698 - +0.6 43,750 + +1.1 43,765 + -0.2 43,765 + +2.6 43,765 + +0.6 43,773 - +0.9 43,773 - +2.6 43,845 + -0.0 43,853 - +1.9 43,895 + B158DRAFT_1908 0.11 +1.9 43,902 + B158DRAFT_1908 0.11 -1.0 43,902 + B158DRAFT_1908 0.11 +1.2 43,902 + B158DRAFT_1908 0.11 -0.3 43,907 + B158DRAFT_1908 0.12 +1.4 43,910 - B158DRAFT_1908 0.12 +0.5 43,915 - B158DRAFT_1908 0.12 +1.4 43,930 + B158DRAFT_1908 0.14 +0.2 43,945 + B158DRAFT_1908 0.15 +0.5 43,975 - B158DRAFT_1908 0.17 -1.9 44,022 - B158DRAFT_1908 0.21 -0.1 44,032 - B158DRAFT_1908 0.22 +0.9 44,043 - B158DRAFT_1908 0.23 +1.9 44,055 - B158DRAFT_1908 0.24 +0.3 44,069 + B158DRAFT_1908 0.25 +0.7 44,080 - B158DRAFT_1908 0.26 +0.9 44,080 - B158DRAFT_1908 0.26 +1.6 44,081 + B158DRAFT_1908 0.26 +0.9 44,086 + B158DRAFT_1908 0.26 +1.6 44,086 + B158DRAFT_1908 0.26 +2.9 44,086 + B158DRAFT_1908 0.26 +0.9 44,094 - B158DRAFT_1908 0.27 +1.3 44,094 - B158DRAFT_1908 0.27 +0.5 44,122 + B158DRAFT_1908 0.29 +0.1 44,124 - B158DRAFT_1908 0.29 +0.9 44,130 - B158DRAFT_1908 0.30 +0.9 44,130 - B158DRAFT_1908 0.30 +2.5 44,201 + B158DRAFT_1908 0.35 +1.1 44,215 + B158DRAFT_1908 0.36 +0.1 44,231 + B158DRAFT_1908 0.38 +1.5 44,238 + B158DRAFT_1908 0.38 +2.2 44,239 - B158DRAFT_1908 0.38 +0.4 44,239 - B158DRAFT_1908 0.38 -0.1 44,285 + B158DRAFT_1908 0.42 +1.2 44,292 + B158DRAFT_1908 0.42 -0.2 44,375 + B158DRAFT_1908 0.49 +0.9 44,375 + B158DRAFT_1908 0.49 +1.0 44,383 - B158DRAFT_1908 0.50 +2.4 44,397 + B158DRAFT_1908 0.51 -0.2 44,499 - B158DRAFT_1908 0.59 +2.2 44,517 - B158DRAFT_1908 0.60 +2.9 44,542 - B158DRAFT_1908 0.62 +0.9 44,605 + B158DRAFT_1908 0.67 +0.3 44,605 + B158DRAFT_1908 0.67 -0.8 44,605 + B158DRAFT_1908 0.67 +0.3 44,605 + B158DRAFT_1908 0.67 +0.3 44,605 + B158DRAFT_1908 0.67 +0.1 44,605 + B158DRAFT_1908 0.67 +0.2 44,605 + B158DRAFT_1908 0.67 +1.3 44,605 + B158DRAFT_1908 0.67 +0.0 44,605 + B158DRAFT_1908 0.67 +1.3 44,605 + B158DRAFT_1908 0.67 +1.3 44,605 + B158DRAFT_1908 0.67 +0.3 44,605 + B158DRAFT_1908 0.67 +0.7 44,605 + B158DRAFT_1908 0.67 +0.1 44,613 - B158DRAFT_1908 0.68 +1.3 44,613 - B158DRAFT_1908 0.68 +0.9 44,613 - B158DRAFT_1908 0.68 +1.6 44,613 - B158DRAFT_1908 0.68 +2.1 44,613 - B158DRAFT_1908 0.68 +1.8 44,613 - B158DRAFT_1908 0.68 +0.6 44,613 - B158DRAFT_1908 0.68 +0.3 44,613 - B158DRAFT_1908 0.68 +1.1 44,613 - B158DRAFT_1908 0.68 +1.1 44,613 - B158DRAFT_1908 0.68 +0.4
Or see this region's nucleotide sequence