Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1208 and B158DRAFT_1209 overlap by 1 nucleotides B158DRAFT_1209 and B158DRAFT_1210 are separated by 33 nucleotides B158DRAFT_1210 and B158DRAFT_1211 are separated by 71 nucleotides B158DRAFT_1211 and B158DRAFT_1212 are separated by 116 nucleotides
B158DRAFT_1208: B158DRAFT_1208 - cystathionine beta-synthase (acetylserine-dependent) (EC 4.2.1.-), at 1,248,502 to 1,249,467
_1208
B158DRAFT_1209: B158DRAFT_1209 - hypothetical protein, at 1,249,467 to 1,249,769
_1209
B158DRAFT_1210: B158DRAFT_1210 - Cystathionine beta-lyases/cystathionine gamma-synthases, at 1,249,803 to 1,250,978
_1210
B158DRAFT_1211: B158DRAFT_1211 - Predicted transcriptional regulators, at 1,251,050 to 1,251,475
_1211
B158DRAFT_1212: B158DRAFT_1212 - Spermidine synthase, at 1,251,592 to 1,252,503
_1212
Position (kb)
1249
1250
1251 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1248.853 kb on + strand, within B158DRAFT_1208 at 1248.856 kb on - strand, within B158DRAFT_1208 at 1248.856 kb on - strand, within B158DRAFT_1208 at 1248.891 kb on + strand, within B158DRAFT_1208 at 1248.893 kb on - strand, within B158DRAFT_1208 at 1249.005 kb on + strand, within B158DRAFT_1208 at 1249.228 kb on - strand, within B158DRAFT_1208 at 1249.282 kb on + strand, within B158DRAFT_1208 at 1249.282 kb on + strand, within B158DRAFT_1208 at 1249.329 kb on + strand, within B158DRAFT_1208 at 1249.342 kb on + strand, within B158DRAFT_1208 at 1249.342 kb on + strand, within B158DRAFT_1208 at 1249.350 kb on - strand, within B158DRAFT_1208 at 1249.352 kb on + strand, within B158DRAFT_1208 at 1249.601 kb on + strand, within B158DRAFT_1209 at 1249.601 kb on + strand, within B158DRAFT_1209 at 1249.637 kb on + strand, within B158DRAFT_1209 at 1249.727 kb on + strand, within B158DRAFT_1209 at 1249.727 kb on + strand, within B158DRAFT_1209 at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.742 kb on + strand at 1249.750 kb on - strand at 1249.750 kb on - strand at 1249.783 kb on + strand at 1249.783 kb on + strand at 1249.783 kb on + strand at 1250.018 kb on + strand, within B158DRAFT_1210 at 1250.058 kb on + strand, within B158DRAFT_1210 at 1250.080 kb on + strand, within B158DRAFT_1210 at 1250.095 kb on - strand, within B158DRAFT_1210 at 1250.187 kb on + strand, within B158DRAFT_1210 at 1250.257 kb on - strand, within B158DRAFT_1210 at 1250.353 kb on - strand, within B158DRAFT_1210 at 1250.597 kb on - strand, within B158DRAFT_1210 at 1250.647 kb on - strand, within B158DRAFT_1210 at 1250.723 kb on + strand, within B158DRAFT_1210 at 1250.731 kb on - strand, within B158DRAFT_1210 at 1250.840 kb on + strand, within B158DRAFT_1210 at 1250.895 kb on + strand at 1250.972 kb on + strand at 1250.972 kb on - strand at 1251.097 kb on + strand, within B158DRAFT_1211 at 1251.105 kb on - strand, within B158DRAFT_1211 at 1251.105 kb on - strand, within B158DRAFT_1211 at 1251.176 kb on + strand, within B158DRAFT_1211 at 1251.203 kb on + strand, within B158DRAFT_1211 at 1251.211 kb on - strand, within B158DRAFT_1211 at 1251.211 kb on - strand, within B158DRAFT_1211 at 1251.211 kb on - strand, within B158DRAFT_1211 at 1251.249 kb on + strand, within B158DRAFT_1211 at 1251.254 kb on + strand, within B158DRAFT_1211 at 1251.256 kb on - strand, within B158DRAFT_1211 at 1251.284 kb on - strand, within B158DRAFT_1211 at 1251.324 kb on + strand, within B158DRAFT_1211 at 1251.324 kb on + strand, within B158DRAFT_1211 at 1251.337 kb on + strand, within B158DRAFT_1211 at 1251.346 kb on + strand, within B158DRAFT_1211 at 1251.354 kb on - strand, within B158DRAFT_1211 at 1251.496 kb on + strand at 1251.548 kb on + strand at 1251.611 kb on + strand at 1251.611 kb on + strand at 1251.611 kb on + strand at 1251.619 kb on - strand at 1251.619 kb on - strand at 1251.619 kb on - strand at 1251.626 kb on + strand at 1251.679 kb on - strand at 1251.685 kb on - strand, within B158DRAFT_1212 at 1251.765 kb on + strand, within B158DRAFT_1212 at 1251.773 kb on - strand, within B158DRAFT_1212 at 1251.911 kb on + strand, within B158DRAFT_1212 at 1251.911 kb on + strand, within B158DRAFT_1212
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 1,248,853 + B158DRAFT_1208 0.36 +3.8 1,248,856 - B158DRAFT_1208 0.37 -0.0 1,248,856 - B158DRAFT_1208 0.37 +1.0 1,248,891 + B158DRAFT_1208 0.40 -0.6 1,248,893 - B158DRAFT_1208 0.40 +1.0 1,249,005 + B158DRAFT_1208 0.52 +1.0 1,249,228 - B158DRAFT_1208 0.75 -1.0 1,249,282 + B158DRAFT_1208 0.81 +1.0 1,249,282 + B158DRAFT_1208 0.81 +1.0 1,249,329 + B158DRAFT_1208 0.86 -0.3 1,249,342 + B158DRAFT_1208 0.87 -0.0 1,249,342 + B158DRAFT_1208 0.87 +0.3 1,249,350 - B158DRAFT_1208 0.88 +2.4 1,249,352 + B158DRAFT_1208 0.88 +0.3 1,249,601 + B158DRAFT_1209 0.44 -1.2 1,249,601 + B158DRAFT_1209 0.44 +0.2 1,249,637 + B158DRAFT_1209 0.56 -0.0 1,249,727 + B158DRAFT_1209 0.86 -1.3 1,249,727 + B158DRAFT_1209 0.86 -0.2 1,249,742 + +0.3 1,249,742 + +0.0 1,249,742 + +0.0 1,249,742 + +0.0 1,249,742 + +0.1 1,249,742 + -0.5 1,249,742 + -0.5 1,249,742 + +0.3 1,249,742 + -0.3 1,249,742 + +0.8 1,249,742 + +0.5 1,249,742 + +0.1 1,249,742 + -0.1 1,249,742 + +0.5 1,249,742 + +0.5 1,249,742 + -0.4 1,249,750 - +1.0 1,249,750 - -1.3 1,249,783 + +0.1 1,249,783 + +0.1 1,249,783 + -0.2 1,250,018 + B158DRAFT_1210 0.18 +1.0 1,250,058 + B158DRAFT_1210 0.22 +1.6 1,250,080 + B158DRAFT_1210 0.24 -0.6 1,250,095 - B158DRAFT_1210 0.25 +1.4 1,250,187 + B158DRAFT_1210 0.33 +0.4 1,250,257 - B158DRAFT_1210 0.39 +3.8 1,250,353 - B158DRAFT_1210 0.47 +1.0 1,250,597 - B158DRAFT_1210 0.68 +1.0 1,250,647 - B158DRAFT_1210 0.72 +3.0 1,250,723 + B158DRAFT_1210 0.78 +1.7 1,250,731 - B158DRAFT_1210 0.79 +2.0 1,250,840 + B158DRAFT_1210 0.88 -0.0 1,250,895 + -1.2 1,250,972 + -0.1 1,250,972 - +0.6 1,251,097 + B158DRAFT_1211 0.11 +0.0 1,251,105 - B158DRAFT_1211 0.13 -0.8 1,251,105 - B158DRAFT_1211 0.13 -0.7 1,251,176 + B158DRAFT_1211 0.30 -0.6 1,251,203 + B158DRAFT_1211 0.36 +0.1 1,251,211 - B158DRAFT_1211 0.38 -0.4 1,251,211 - B158DRAFT_1211 0.38 -0.5 1,251,211 - B158DRAFT_1211 0.38 -0.4 1,251,249 + B158DRAFT_1211 0.47 +0.3 1,251,254 + B158DRAFT_1211 0.48 -0.9 1,251,256 - B158DRAFT_1211 0.48 -0.4 1,251,284 - B158DRAFT_1211 0.55 +0.3 1,251,324 + B158DRAFT_1211 0.64 -2.3 1,251,324 + B158DRAFT_1211 0.64 -0.2 1,251,337 + B158DRAFT_1211 0.67 +1.2 1,251,346 + B158DRAFT_1211 0.69 +1.2 1,251,354 - B158DRAFT_1211 0.71 -0.4 1,251,496 + -0.3 1,251,548 + -0.3 1,251,611 + +0.1 1,251,611 + -0.3 1,251,611 + -0.4 1,251,619 - -0.2 1,251,619 - +0.4 1,251,619 - -0.0 1,251,626 + -0.0 1,251,679 - +0.4 1,251,685 - B158DRAFT_1212 0.10 +0.2 1,251,765 + B158DRAFT_1212 0.19 +0.1 1,251,773 - B158DRAFT_1212 0.20 +0.1 1,251,911 + B158DRAFT_1212 0.35 +0.5 1,251,911 + B158DRAFT_1212 0.35 -0.1
Or see this region's nucleotide sequence