Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0670 and B158DRAFT_0671 overlap by 8 nucleotides B158DRAFT_0671 and B158DRAFT_0672 are separated by 18 nucleotides B158DRAFT_0672 and B158DRAFT_0674 are separated by 285 nucleotides
B158DRAFT_0670: B158DRAFT_0670 - Uncharacterized protein required for cytochrome oxidase assembly, at 672,866 to 673,975
_0670
B158DRAFT_0671: B158DRAFT_0671 - protoheme IX farnesyltransferase, at 673,968 to 674,915
_0671
B158DRAFT_0672: B158DRAFT_0672 - Uncharacterized protein SCO1/SenC/PrrC, involved in biogenesis of respiratory and photosynthetic systems, at 674,934 to 675,584
_0672
B158DRAFT_0674: B158DRAFT_0674 - putative efflux protein, MATE family, at 675,870 to 677,246
_0674
Position (kb)
673
674
675 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.998 kb on - strand, within B158DRAFT_0670 at 673.017 kb on + strand, within B158DRAFT_0670 at 673.070 kb on + strand, within B158DRAFT_0670 at 673.104 kb on + strand, within B158DRAFT_0670 at 673.118 kb on + strand, within B158DRAFT_0670 at 673.128 kb on + strand, within B158DRAFT_0670 at 673.169 kb on + strand, within B158DRAFT_0670 at 673.202 kb on - strand, within B158DRAFT_0670 at 673.270 kb on - strand, within B158DRAFT_0670 at 673.299 kb on - strand, within B158DRAFT_0670 at 673.412 kb on - strand, within B158DRAFT_0670 at 673.412 kb on - strand, within B158DRAFT_0670 at 673.412 kb on - strand, within B158DRAFT_0670 at 673.412 kb on - strand, within B158DRAFT_0670 at 673.412 kb on - strand, within B158DRAFT_0670 at 673.550 kb on + strand, within B158DRAFT_0670 at 673.558 kb on - strand, within B158DRAFT_0670 at 673.567 kb on + strand, within B158DRAFT_0670 at 673.575 kb on - strand, within B158DRAFT_0670 at 673.606 kb on + strand, within B158DRAFT_0670 at 673.606 kb on + strand, within B158DRAFT_0670 at 673.618 kb on + strand, within B158DRAFT_0670 at 673.621 kb on - strand, within B158DRAFT_0670 at 673.725 kb on + strand, within B158DRAFT_0670 at 673.771 kb on - strand, within B158DRAFT_0670 at 673.771 kb on - strand, within B158DRAFT_0670 at 673.771 kb on - strand, within B158DRAFT_0670 at 673.771 kb on - strand, within B158DRAFT_0670 at 673.771 kb on - strand, within B158DRAFT_0670 at 673.771 kb on - strand, within B158DRAFT_0670 at 673.789 kb on - strand, within B158DRAFT_0670 at 673.798 kb on + strand, within B158DRAFT_0670 at 673.798 kb on + strand, within B158DRAFT_0670 at 673.798 kb on + strand, within B158DRAFT_0670 at 673.806 kb on - strand, within B158DRAFT_0670 at 673.806 kb on - strand, within B158DRAFT_0670 at 673.806 kb on - strand, within B158DRAFT_0670 at 673.806 kb on - strand, within B158DRAFT_0670 at 673.959 kb on + strand at 674.024 kb on - strand at 674.063 kb on + strand, within B158DRAFT_0671 at 674.100 kb on - strand, within B158DRAFT_0671 at 674.116 kb on - strand, within B158DRAFT_0671 at 674.162 kb on - strand, within B158DRAFT_0671 at 674.169 kb on + strand, within B158DRAFT_0671 at 674.177 kb on - strand, within B158DRAFT_0671 at 674.214 kb on + strand, within B158DRAFT_0671 at 674.231 kb on + strand, within B158DRAFT_0671 at 674.254 kb on - strand, within B158DRAFT_0671 at 674.373 kb on + strand, within B158DRAFT_0671 at 674.374 kb on + strand, within B158DRAFT_0671 at 674.397 kb on + strand, within B158DRAFT_0671 at 674.527 kb on - strand, within B158DRAFT_0671 at 674.527 kb on - strand, within B158DRAFT_0671 at 674.766 kb on - strand, within B158DRAFT_0671 at 674.830 kb on + strand at 674.838 kb on - strand at 674.838 kb on - strand at 675.105 kb on + strand, within B158DRAFT_0672 at 675.172 kb on + strand, within B158DRAFT_0672 at 675.180 kb on - strand, within B158DRAFT_0672 at 675.180 kb on - strand, within B158DRAFT_0672 at 675.244 kb on + strand, within B158DRAFT_0672 at 675.341 kb on + strand, within B158DRAFT_0672 at 675.479 kb on - strand, within B158DRAFT_0672 at 675.492 kb on + strand, within B158DRAFT_0672 at 675.492 kb on + strand, within B158DRAFT_0672 at 675.500 kb on - strand, within B158DRAFT_0672 at 675.500 kb on - strand, within B158DRAFT_0672 at 675.500 kb on - strand, within B158DRAFT_0672 at 675.519 kb on + strand, within B158DRAFT_0672 at 675.519 kb on + strand, within B158DRAFT_0672 at 675.527 kb on - strand at 675.527 kb on - strand at 675.535 kb on + strand at 675.550 kb on - strand at 675.586 kb on - strand at 675.785 kb on - strand at 675.850 kb on - strand at 675.869 kb on - strand at 675.883 kb on - strand at 675.903 kb on + strand at 675.913 kb on + strand at 675.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 672,970 - +0.5 672,970 - +0.8 672,970 - +0.4 672,970 - +1.2 672,970 - -0.6 672,970 - +0.6 672,970 - +0.5 672,998 - B158DRAFT_0670 0.12 +0.8 673,017 + B158DRAFT_0670 0.14 +1.4 673,070 + B158DRAFT_0670 0.18 +1.3 673,104 + B158DRAFT_0670 0.21 +1.8 673,118 + B158DRAFT_0670 0.23 +1.7 673,128 + B158DRAFT_0670 0.24 +0.9 673,169 + B158DRAFT_0670 0.27 +1.8 673,202 - B158DRAFT_0670 0.30 +1.0 673,270 - B158DRAFT_0670 0.36 -0.2 673,299 - B158DRAFT_0670 0.39 +0.8 673,412 - B158DRAFT_0670 0.49 -0.2 673,412 - B158DRAFT_0670 0.49 +2.1 673,412 - B158DRAFT_0670 0.49 +1.4 673,412 - B158DRAFT_0670 0.49 +1.8 673,412 - B158DRAFT_0670 0.49 +1.2 673,550 + B158DRAFT_0670 0.62 +1.4 673,558 - B158DRAFT_0670 0.62 +1.7 673,567 + B158DRAFT_0670 0.63 +0.8 673,575 - B158DRAFT_0670 0.64 -0.8 673,606 + B158DRAFT_0670 0.67 +0.7 673,606 + B158DRAFT_0670 0.67 +1.2 673,618 + B158DRAFT_0670 0.68 -0.8 673,621 - B158DRAFT_0670 0.68 +1.9 673,725 + B158DRAFT_0670 0.77 +0.8 673,771 - B158DRAFT_0670 0.82 +0.7 673,771 - B158DRAFT_0670 0.82 +3.4 673,771 - B158DRAFT_0670 0.82 +1.1 673,771 - B158DRAFT_0670 0.82 +2.0 673,771 - B158DRAFT_0670 0.82 +2.3 673,771 - B158DRAFT_0670 0.82 +1.3 673,789 - B158DRAFT_0670 0.83 +1.0 673,798 + B158DRAFT_0670 0.84 -0.2 673,798 + B158DRAFT_0670 0.84 +0.8 673,798 + B158DRAFT_0670 0.84 +1.3 673,806 - B158DRAFT_0670 0.85 +2.8 673,806 - B158DRAFT_0670 0.85 +1.4 673,806 - B158DRAFT_0670 0.85 +1.0 673,806 - B158DRAFT_0670 0.85 +1.4 673,959 + +0.3 674,024 - +0.8 674,063 + B158DRAFT_0671 0.10 -0.2 674,100 - B158DRAFT_0671 0.14 +2.1 674,116 - B158DRAFT_0671 0.16 +1.2 674,162 - B158DRAFT_0671 0.20 +0.2 674,169 + B158DRAFT_0671 0.21 +0.9 674,177 - B158DRAFT_0671 0.22 +1.4 674,214 + B158DRAFT_0671 0.26 +0.5 674,231 + B158DRAFT_0671 0.28 +0.8 674,254 - B158DRAFT_0671 0.30 +0.8 674,373 + B158DRAFT_0671 0.43 +0.6 674,374 + B158DRAFT_0671 0.43 +0.4 674,397 + B158DRAFT_0671 0.45 -0.2 674,527 - B158DRAFT_0671 0.59 +2.3 674,527 - B158DRAFT_0671 0.59 +1.6 674,766 - B158DRAFT_0671 0.84 +1.2 674,830 + +0.0 674,838 - +1.5 674,838 - +1.5 675,105 + B158DRAFT_0672 0.26 +0.2 675,172 + B158DRAFT_0672 0.37 +1.6 675,180 - B158DRAFT_0672 0.38 +2.1 675,180 - B158DRAFT_0672 0.38 +1.2 675,244 + B158DRAFT_0672 0.48 +0.4 675,341 + B158DRAFT_0672 0.63 -1.8 675,479 - B158DRAFT_0672 0.84 +1.8 675,492 + B158DRAFT_0672 0.86 +3.4 675,492 + B158DRAFT_0672 0.86 +2.8 675,500 - B158DRAFT_0672 0.87 -0.2 675,500 - B158DRAFT_0672 0.87 +0.2 675,500 - B158DRAFT_0672 0.87 +0.6 675,519 + B158DRAFT_0672 0.90 +1.4 675,519 + B158DRAFT_0672 0.90 +2.0 675,527 - +0.4 675,527 - +0.1 675,535 + +0.8 675,550 - +1.2 675,586 - -0.0 675,785 - -0.1 675,850 - +2.4 675,869 - -0.7 675,883 - +0.0 675,903 + +0.2 675,913 + -0.3 675,913 + +0.4
Or see this region's nucleotide sequence