Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0666 and B158DRAFT_0667 overlap by 44 nucleotides B158DRAFT_0667 and B158DRAFT_0668 are separated by 131 nucleotides B158DRAFT_0668 and B158DRAFT_0669 overlap by 20 nucleotides B158DRAFT_0669 and B158DRAFT_0670 are separated by 13 nucleotides
B158DRAFT_0666: B158DRAFT_0666 - Protein of unknown function (DUF3667)., at 670,050 to 671,102
_0666
B158DRAFT_0667: B158DRAFT_0667 - Protein of unknown function (DUF2909)., at 671,059 to 671,343
_0667
B158DRAFT_0668: B158DRAFT_0668 - Uncharacterized conserved protein, at 671,475 to 672,260
_0668
B158DRAFT_0669: B158DRAFT_0669 - hypothetical protein, at 672,241 to 672,852
_0669
B158DRAFT_0670: B158DRAFT_0670 - Uncharacterized protein required for cytochrome oxidase assembly, at 672,866 to 673,975
_0670
Position (kb) 671
672
673 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 670.478 kb on + strand, within B158DRAFT_0666 at 670.550 kb on + strand, within B158DRAFT_0666 at 670.594 kb on + strand, within B158DRAFT_0666 at 670.608 kb on + strand, within B158DRAFT_0666 at 670.642 kb on + strand, within B158DRAFT_0666 at 670.642 kb on + strand, within B158DRAFT_0666 at 670.642 kb on + strand, within B158DRAFT_0666 at 670.642 kb on + strand, within B158DRAFT_0666 at 670.642 kb on + strand, within B158DRAFT_0666 at 670.650 kb on - strand, within B158DRAFT_0666 at 670.650 kb on - strand, within B158DRAFT_0666 at 670.650 kb on - strand, within B158DRAFT_0666 at 670.650 kb on - strand, within B158DRAFT_0666 at 670.650 kb on - strand, within B158DRAFT_0666 at 670.676 kb on + strand, within B158DRAFT_0666 at 670.676 kb on + strand, within B158DRAFT_0666 at 670.676 kb on + strand, within B158DRAFT_0666 at 670.676 kb on + strand, within B158DRAFT_0666 at 670.684 kb on - strand, within B158DRAFT_0666 at 670.684 kb on - strand, within B158DRAFT_0666 at 670.684 kb on - strand, within B158DRAFT_0666 at 670.691 kb on - strand, within B158DRAFT_0666 at 670.696 kb on - strand, within B158DRAFT_0666 at 670.715 kb on + strand, within B158DRAFT_0666 at 670.722 kb on + strand, within B158DRAFT_0666 at 670.730 kb on - strand, within B158DRAFT_0666 at 670.731 kb on + strand, within B158DRAFT_0666 at 670.844 kb on + strand, within B158DRAFT_0666 at 670.852 kb on - strand, within B158DRAFT_0666 at 670.915 kb on - strand, within B158DRAFT_0666 at 670.920 kb on - strand, within B158DRAFT_0666 at 670.989 kb on - strand, within B158DRAFT_0666 at 671.025 kb on + strand at 671.074 kb on - strand at 671.084 kb on - strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.086 kb on + strand at 671.094 kb on - strand at 671.094 kb on - strand at 671.094 kb on - strand at 671.110 kb on + strand, within B158DRAFT_0667 at 671.131 kb on + strand, within B158DRAFT_0667 at 671.144 kb on - strand, within B158DRAFT_0667 at 671.160 kb on + strand, within B158DRAFT_0667 at 671.160 kb on + strand, within B158DRAFT_0667 at 671.202 kb on - strand, within B158DRAFT_0667 at 671.229 kb on - strand, within B158DRAFT_0667 at 671.261 kb on + strand, within B158DRAFT_0667 at 671.485 kb on - strand at 671.510 kb on + strand at 671.515 kb on + strand at 671.523 kb on - strand at 671.526 kb on + strand at 671.602 kb on - strand, within B158DRAFT_0668 at 671.651 kb on + strand, within B158DRAFT_0668 at 671.656 kb on + strand, within B158DRAFT_0668 at 671.688 kb on - strand, within B158DRAFT_0668 at 671.694 kb on + strand, within B158DRAFT_0668 at 671.704 kb on + strand, within B158DRAFT_0668 at 671.704 kb on + strand, within B158DRAFT_0668 at 671.712 kb on - strand, within B158DRAFT_0668 at 671.712 kb on - strand, within B158DRAFT_0668 at 671.712 kb on - strand, within B158DRAFT_0668 at 671.712 kb on - strand, within B158DRAFT_0668 at 671.713 kb on - strand, within B158DRAFT_0668 at 671.727 kb on + strand, within B158DRAFT_0668 at 671.764 kb on + strand, within B158DRAFT_0668 at 671.771 kb on - strand, within B158DRAFT_0668 at 671.772 kb on + strand, within B158DRAFT_0668 at 671.777 kb on + strand, within B158DRAFT_0668 at 671.802 kb on - strand, within B158DRAFT_0668 at 671.846 kb on + strand, within B158DRAFT_0668 at 671.913 kb on + strand, within B158DRAFT_0668 at 671.921 kb on - strand, within B158DRAFT_0668 at 671.921 kb on - strand, within B158DRAFT_0668 at 671.928 kb on + strand, within B158DRAFT_0668 at 671.928 kb on - strand, within B158DRAFT_0668 at 671.950 kb on + strand, within B158DRAFT_0668 at 672.057 kb on - strand, within B158DRAFT_0668 at 672.098 kb on + strand, within B158DRAFT_0668 at 672.100 kb on - strand, within B158DRAFT_0668 at 672.104 kb on - strand, within B158DRAFT_0668 at 672.141 kb on + strand, within B158DRAFT_0668 at 672.168 kb on + strand, within B158DRAFT_0668 at 672.235 kb on + strand at 672.312 kb on - strand, within B158DRAFT_0669 at 672.348 kb on + strand, within B158DRAFT_0669 at 672.368 kb on - strand, within B158DRAFT_0669 at 672.386 kb on + strand, within B158DRAFT_0669 at 672.409 kb on - strand, within B158DRAFT_0669 at 672.466 kb on - strand, within B158DRAFT_0669 at 672.478 kb on + strand, within B158DRAFT_0669 at 672.545 kb on + strand, within B158DRAFT_0669 at 672.545 kb on + strand, within B158DRAFT_0669 at 672.550 kb on + strand, within B158DRAFT_0669 at 672.550 kb on + strand, within B158DRAFT_0669 at 672.550 kb on + strand, within B158DRAFT_0669 at 672.550 kb on + strand, within B158DRAFT_0669 at 672.551 kb on + strand, within B158DRAFT_0669 at 672.553 kb on - strand, within B158DRAFT_0669 at 672.553 kb on - strand, within B158DRAFT_0669 at 672.553 kb on - strand, within B158DRAFT_0669 at 672.558 kb on - strand, within B158DRAFT_0669 at 672.558 kb on - strand, within B158DRAFT_0669 at 672.637 kb on + strand, within B158DRAFT_0669 at 672.637 kb on + strand, within B158DRAFT_0669 at 672.637 kb on + strand, within B158DRAFT_0669 at 672.637 kb on + strand, within B158DRAFT_0669 at 672.637 kb on + strand, within B158DRAFT_0669 at 672.637 kb on + strand, within B158DRAFT_0669 at 672.645 kb on - strand, within B158DRAFT_0669 at 672.645 kb on - strand, within B158DRAFT_0669 at 672.645 kb on - strand, within B158DRAFT_0669 at 672.645 kb on - strand, within B158DRAFT_0669 at 672.645 kb on - strand, within B158DRAFT_0669 at 672.645 kb on - strand, within B158DRAFT_0669 at 672.685 kb on + strand, within B158DRAFT_0669 at 672.688 kb on - strand, within B158DRAFT_0669 at 672.690 kb on + strand, within B158DRAFT_0669 at 672.705 kb on + strand, within B158DRAFT_0669 at 672.798 kb on + strand at 672.806 kb on - strand at 672.808 kb on - strand at 672.813 kb on - strand at 672.832 kb on + strand at 672.837 kb on + strand at 672.837 kb on + strand at 672.837 kb on + strand at 672.837 kb on + strand at 672.947 kb on + strand at 672.962 kb on + strand at 672.962 kb on + strand at 672.962 kb on + strand at 672.962 kb on + strand at 672.962 kb on + strand at 672.962 kb on + strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.970 kb on - strand at 672.998 kb on - strand, within B158DRAFT_0670 at 673.017 kb on + strand, within B158DRAFT_0670 at 673.070 kb on + strand, within B158DRAFT_0670 at 673.104 kb on + strand, within B158DRAFT_0670 at 673.118 kb on + strand, within B158DRAFT_0670 at 673.128 kb on + strand, within B158DRAFT_0670 at 673.169 kb on + strand, within B158DRAFT_0670 at 673.202 kb on - strand, within B158DRAFT_0670
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 670,478 + B158DRAFT_0666 0.41 -0.2 670,550 + B158DRAFT_0666 0.47 -0.4 670,594 + B158DRAFT_0666 0.52 -0.7 670,608 + B158DRAFT_0666 0.53 +0.6 670,642 + B158DRAFT_0666 0.56 +0.7 670,642 + B158DRAFT_0666 0.56 +0.4 670,642 + B158DRAFT_0666 0.56 -0.3 670,642 + B158DRAFT_0666 0.56 -0.7 670,642 + B158DRAFT_0666 0.56 -0.0 670,650 - B158DRAFT_0666 0.57 +0.6 670,650 - B158DRAFT_0666 0.57 -0.1 670,650 - B158DRAFT_0666 0.57 -2.4 670,650 - B158DRAFT_0666 0.57 -0.7 670,650 - B158DRAFT_0666 0.57 -0.5 670,676 + B158DRAFT_0666 0.59 +0.1 670,676 + B158DRAFT_0666 0.59 +0.1 670,676 + B158DRAFT_0666 0.59 +0.0 670,676 + B158DRAFT_0666 0.59 +0.4 670,684 - B158DRAFT_0666 0.60 -0.1 670,684 - B158DRAFT_0666 0.60 -0.0 670,684 - B158DRAFT_0666 0.60 +0.0 670,691 - B158DRAFT_0666 0.61 -0.7 670,696 - B158DRAFT_0666 0.61 +0.0 670,715 + B158DRAFT_0666 0.63 -0.2 670,722 + B158DRAFT_0666 0.64 -0.3 670,730 - B158DRAFT_0666 0.65 -0.4 670,731 + B158DRAFT_0666 0.65 -0.2 670,844 + B158DRAFT_0666 0.75 -0.0 670,852 - B158DRAFT_0666 0.76 -0.4 670,915 - B158DRAFT_0666 0.82 -0.5 670,920 - B158DRAFT_0666 0.83 -0.3 670,989 - B158DRAFT_0666 0.89 -0.1 671,025 + -0.1 671,074 - -1.9 671,084 - -0.1 671,086 + +0.4 671,086 + +0.0 671,086 + -0.3 671,086 + +0.1 671,086 + -0.0 671,086 + +0.2 671,086 + -0.5 671,086 + -0.2 671,086 + +0.0 671,094 - +0.3 671,094 - +0.2 671,094 - -0.1 671,110 + B158DRAFT_0667 0.18 -0.5 671,131 + B158DRAFT_0667 0.25 -0.4 671,144 - B158DRAFT_0667 0.30 -0.2 671,160 + B158DRAFT_0667 0.35 +0.2 671,160 + B158DRAFT_0667 0.35 -0.1 671,202 - B158DRAFT_0667 0.50 +0.0 671,229 - B158DRAFT_0667 0.60 -0.1 671,261 + B158DRAFT_0667 0.71 -0.7 671,485 - +0.8 671,510 + -2.5 671,515 + +0.2 671,523 - +1.2 671,526 + -0.2 671,602 - B158DRAFT_0668 0.16 +0.2 671,651 + B158DRAFT_0668 0.22 +1.3 671,656 + B158DRAFT_0668 0.23 +1.8 671,688 - B158DRAFT_0668 0.27 +1.1 671,694 + B158DRAFT_0668 0.28 +0.8 671,704 + B158DRAFT_0668 0.29 +0.4 671,704 + B158DRAFT_0668 0.29 -1.7 671,712 - B158DRAFT_0668 0.30 +1.3 671,712 - B158DRAFT_0668 0.30 -0.2 671,712 - B158DRAFT_0668 0.30 +1.0 671,712 - B158DRAFT_0668 0.30 +1.4 671,713 - B158DRAFT_0668 0.30 +2.4 671,727 + B158DRAFT_0668 0.32 +0.8 671,764 + B158DRAFT_0668 0.37 +0.8 671,771 - B158DRAFT_0668 0.38 +0.1 671,772 + B158DRAFT_0668 0.38 +1.5 671,777 + B158DRAFT_0668 0.38 +0.8 671,802 - B158DRAFT_0668 0.42 +3.0 671,846 + B158DRAFT_0668 0.47 +1.8 671,913 + B158DRAFT_0668 0.56 -0.8 671,921 - B158DRAFT_0668 0.57 +2.2 671,921 - B158DRAFT_0668 0.57 +2.1 671,928 + B158DRAFT_0668 0.58 +0.8 671,928 - B158DRAFT_0668 0.58 +0.2 671,950 + B158DRAFT_0668 0.60 +1.4 672,057 - B158DRAFT_0668 0.74 +0.3 672,098 + B158DRAFT_0668 0.79 +0.8 672,100 - B158DRAFT_0668 0.80 +0.6 672,104 - B158DRAFT_0668 0.80 +2.8 672,141 + B158DRAFT_0668 0.85 +1.4 672,168 + B158DRAFT_0668 0.88 +2.1 672,235 + -0.2 672,312 - B158DRAFT_0669 0.12 +1.6 672,348 + B158DRAFT_0669 0.17 +0.8 672,368 - B158DRAFT_0669 0.21 +0.6 672,386 + B158DRAFT_0669 0.24 +1.5 672,409 - B158DRAFT_0669 0.27 +0.6 672,466 - B158DRAFT_0669 0.37 +0.5 672,478 + B158DRAFT_0669 0.39 +1.8 672,545 + B158DRAFT_0669 0.50 +0.1 672,545 + B158DRAFT_0669 0.50 +0.9 672,550 + B158DRAFT_0669 0.50 +1.8 672,550 + B158DRAFT_0669 0.50 +2.1 672,550 + B158DRAFT_0669 0.50 +1.1 672,550 + B158DRAFT_0669 0.50 +2.2 672,551 + B158DRAFT_0669 0.51 +0.9 672,553 - B158DRAFT_0669 0.51 +0.0 672,553 - B158DRAFT_0669 0.51 -0.2 672,553 - B158DRAFT_0669 0.51 +0.9 672,558 - B158DRAFT_0669 0.52 +0.6 672,558 - B158DRAFT_0669 0.52 +1.3 672,637 + B158DRAFT_0669 0.65 +1.5 672,637 + B158DRAFT_0669 0.65 +0.4 672,637 + B158DRAFT_0669 0.65 +1.7 672,637 + B158DRAFT_0669 0.65 +1.5 672,637 + B158DRAFT_0669 0.65 +0.8 672,637 + B158DRAFT_0669 0.65 +2.8 672,645 - B158DRAFT_0669 0.66 +0.6 672,645 - B158DRAFT_0669 0.66 +0.8 672,645 - B158DRAFT_0669 0.66 +0.5 672,645 - B158DRAFT_0669 0.66 +1.1 672,645 - B158DRAFT_0669 0.66 +0.2 672,645 - B158DRAFT_0669 0.66 +0.8 672,685 + B158DRAFT_0669 0.73 +1.0 672,688 - B158DRAFT_0669 0.73 +1.4 672,690 + B158DRAFT_0669 0.73 +1.8 672,705 + B158DRAFT_0669 0.76 +0.5 672,798 + +1.1 672,806 - +1.4 672,808 - -0.7 672,813 - -0.2 672,832 + -0.1 672,837 + +0.2 672,837 + +0.9 672,837 + +0.2 672,837 + +0.3 672,947 + +1.8 672,962 + +0.8 672,962 + -0.2 672,962 + +1.8 672,962 + +0.4 672,962 + -1.1 672,962 + +0.8 672,970 - +0.5 672,970 - +0.8 672,970 - +0.4 672,970 - +1.2 672,970 - -0.6 672,970 - +0.6 672,970 - +0.5 672,998 - B158DRAFT_0670 0.12 +0.8 673,017 + B158DRAFT_0670 0.14 +1.4 673,070 + B158DRAFT_0670 0.18 +1.3 673,104 + B158DRAFT_0670 0.21 +1.8 673,118 + B158DRAFT_0670 0.23 +1.7 673,128 + B158DRAFT_0670 0.24 +0.9 673,169 + B158DRAFT_0670 0.27 +1.8 673,202 - B158DRAFT_0670 0.30 +1.0
Or see this region's nucleotide sequence