Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0400 and B158DRAFT_0401 are separated by 85 nucleotides B158DRAFT_0401 and B158DRAFT_0402 are separated by 213 nucleotides
B158DRAFT_0400: B158DRAFT_0400 - DNA protecting protein DprA, at 406,069 to 407,160
_0400
B158DRAFT_0401: B158DRAFT_0401 - Uncharacterized protein conserved in bacteria, at 407,246 to 407,725
_0401
B158DRAFT_0402: B158DRAFT_0402 - Thiol-disulfide isomerase and thioredoxins, at 407,939 to 408,781
_0402
Position (kb)
407
408 Strain fitness (log2 ratio)
-2
-1
0
1 at 406.247 kb on + strand, within B158DRAFT_0400 at 406.255 kb on - strand, within B158DRAFT_0400 at 406.261 kb on - strand, within B158DRAFT_0400 at 406.267 kb on + strand, within B158DRAFT_0400 at 406.305 kb on - strand, within B158DRAFT_0400 at 406.330 kb on + strand, within B158DRAFT_0400 at 406.380 kb on + strand, within B158DRAFT_0400 at 406.388 kb on - strand, within B158DRAFT_0400 at 406.388 kb on - strand, within B158DRAFT_0400 at 406.388 kb on - strand, within B158DRAFT_0400 at 406.388 kb on - strand, within B158DRAFT_0400 at 406.388 kb on - strand, within B158DRAFT_0400 at 406.492 kb on - strand, within B158DRAFT_0400 at 406.495 kb on + strand, within B158DRAFT_0400 at 406.495 kb on + strand, within B158DRAFT_0400 at 406.503 kb on - strand, within B158DRAFT_0400 at 406.503 kb on - strand, within B158DRAFT_0400 at 406.521 kb on - strand, within B158DRAFT_0400 at 406.546 kb on + strand, within B158DRAFT_0400 at 406.551 kb on + strand, within B158DRAFT_0400 at 406.551 kb on + strand, within B158DRAFT_0400 at 406.554 kb on - strand, within B158DRAFT_0400 at 406.559 kb on - strand, within B158DRAFT_0400 at 406.559 kb on - strand, within B158DRAFT_0400 at 406.576 kb on - strand, within B158DRAFT_0400 at 406.593 kb on + strand, within B158DRAFT_0400 at 406.642 kb on + strand, within B158DRAFT_0400 at 406.677 kb on - strand, within B158DRAFT_0400 at 406.682 kb on - strand, within B158DRAFT_0400 at 406.682 kb on - strand, within B158DRAFT_0400 at 406.735 kb on - strand, within B158DRAFT_0400 at 406.757 kb on - strand, within B158DRAFT_0400 at 406.806 kb on + strand, within B158DRAFT_0400 at 406.808 kb on + strand, within B158DRAFT_0400 at 406.808 kb on + strand, within B158DRAFT_0400 at 406.808 kb on + strand, within B158DRAFT_0400 at 406.816 kb on - strand, within B158DRAFT_0400 at 406.816 kb on - strand, within B158DRAFT_0400 at 406.816 kb on - strand, within B158DRAFT_0400 at 406.832 kb on + strand, within B158DRAFT_0400 at 406.851 kb on + strand, within B158DRAFT_0400 at 406.851 kb on + strand, within B158DRAFT_0400 at 406.851 kb on + strand, within B158DRAFT_0400 at 406.851 kb on + strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 406.859 kb on - strand, within B158DRAFT_0400 at 407.048 kb on + strand, within B158DRAFT_0400 at 407.048 kb on + strand, within B158DRAFT_0400 at 407.056 kb on - strand at 407.056 kb on - strand at 407.056 kb on - strand at 407.056 kb on - strand at 407.056 kb on - strand at 407.056 kb on - strand at 407.066 kb on - strand at 407.087 kb on + strand at 407.215 kb on + strand at 407.223 kb on - strand at 407.223 kb on - strand at 407.229 kb on + strand at 407.229 kb on + strand at 407.229 kb on + strand at 407.229 kb on + strand at 407.229 kb on + strand at 407.229 kb on + strand at 407.237 kb on - strand at 407.237 kb on - strand at 407.237 kb on - strand at 407.237 kb on - strand at 407.237 kb on - strand at 407.237 kb on - strand at 407.237 kb on - strand at 407.255 kb on + strand at 407.418 kb on + strand, within B158DRAFT_0401 at 407.453 kb on + strand, within B158DRAFT_0401 at 407.477 kb on + strand, within B158DRAFT_0401 at 407.504 kb on + strand, within B158DRAFT_0401 at 407.510 kb on + strand, within B158DRAFT_0401 at 407.511 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.523 kb on + strand, within B158DRAFT_0401 at 407.526 kb on - strand, within B158DRAFT_0401 at 407.531 kb on - strand, within B158DRAFT_0401 at 407.531 kb on - strand, within B158DRAFT_0401 at 407.531 kb on - strand, within B158DRAFT_0401 at 407.531 kb on - strand, within B158DRAFT_0401 at 407.531 kb on - strand, within B158DRAFT_0401 at 407.712 kb on - strand at 407.742 kb on + strand at 407.745 kb on - strand at 407.755 kb on + strand at 407.755 kb on + strand at 407.755 kb on + strand at 407.755 kb on + strand at 407.763 kb on - strand at 407.763 kb on - strand at 407.777 kb on + strand at 407.777 kb on + strand at 407.785 kb on - strand at 407.786 kb on - strand at 407.859 kb on - strand at 407.859 kb on - strand at 407.895 kb on + strand at 407.957 kb on + strand at 408.012 kb on + strand at 408.012 kb on + strand at 408.106 kb on + strand, within B158DRAFT_0402 at 408.106 kb on + strand, within B158DRAFT_0402 at 408.119 kb on + strand, within B158DRAFT_0402 at 408.119 kb on + strand, within B158DRAFT_0402 at 408.119 kb on + strand, within B158DRAFT_0402 at 408.127 kb on - strand, within B158DRAFT_0402 at 408.127 kb on - strand, within B158DRAFT_0402 at 408.127 kb on - strand, within B158DRAFT_0402 at 408.132 kb on - strand, within B158DRAFT_0402 at 408.132 kb on - strand, within B158DRAFT_0402 at 408.209 kb on + strand, within B158DRAFT_0402 at 408.219 kb on + strand, within B158DRAFT_0402 at 408.260 kb on + strand, within B158DRAFT_0402 at 408.268 kb on - strand, within B158DRAFT_0402 at 408.289 kb on - strand, within B158DRAFT_0402 at 408.299 kb on - strand, within B158DRAFT_0402 at 408.328 kb on + strand, within B158DRAFT_0402 at 408.328 kb on + strand, within B158DRAFT_0402 at 408.328 kb on + strand, within B158DRAFT_0402 at 408.328 kb on + strand, within B158DRAFT_0402 at 408.336 kb on - strand, within B158DRAFT_0402 at 408.336 kb on - strand, within B158DRAFT_0402 at 408.336 kb on - strand, within B158DRAFT_0402 at 408.338 kb on + strand, within B158DRAFT_0402 at 408.338 kb on + strand, within B158DRAFT_0402 at 408.346 kb on - strand, within B158DRAFT_0402 at 408.381 kb on - strand, within B158DRAFT_0402 at 408.399 kb on + strand, within B158DRAFT_0402 at 408.404 kb on + strand, within B158DRAFT_0402 at 408.407 kb on - strand, within B158DRAFT_0402 at 408.414 kb on + strand, within B158DRAFT_0402 at 408.422 kb on - strand, within B158DRAFT_0402 at 408.448 kb on - strand, within B158DRAFT_0402 at 408.530 kb on + strand, within B158DRAFT_0402 at 408.537 kb on + strand, within B158DRAFT_0402 at 408.583 kb on + strand, within B158DRAFT_0402 at 408.589 kb on + strand, within B158DRAFT_0402 at 408.591 kb on - strand, within B158DRAFT_0402 at 408.597 kb on - strand, within B158DRAFT_0402 at 408.611 kb on + strand, within B158DRAFT_0402 at 408.611 kb on + strand, within B158DRAFT_0402 at 408.619 kb on - strand, within B158DRAFT_0402 at 408.619 kb on - strand, within B158DRAFT_0402 at 408.619 kb on - strand, within B158DRAFT_0402
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 406,247 + B158DRAFT_0400 0.16 -0.4 406,255 - B158DRAFT_0400 0.17 +0.2 406,261 - B158DRAFT_0400 0.18 +0.1 406,267 + B158DRAFT_0400 0.18 -0.5 406,305 - B158DRAFT_0400 0.22 -0.5 406,330 + B158DRAFT_0400 0.24 -0.3 406,380 + B158DRAFT_0400 0.28 +0.0 406,388 - B158DRAFT_0400 0.29 -2.3 406,388 - B158DRAFT_0400 0.29 -0.7 406,388 - B158DRAFT_0400 0.29 -0.1 406,388 - B158DRAFT_0400 0.29 +0.4 406,388 - B158DRAFT_0400 0.29 -0.1 406,492 - B158DRAFT_0400 0.39 -0.4 406,495 + B158DRAFT_0400 0.39 -0.2 406,495 + B158DRAFT_0400 0.39 +0.0 406,503 - B158DRAFT_0400 0.40 +0.1 406,503 - B158DRAFT_0400 0.40 +0.1 406,521 - B158DRAFT_0400 0.41 -0.1 406,546 + B158DRAFT_0400 0.44 -0.1 406,551 + B158DRAFT_0400 0.44 -1.0 406,551 + B158DRAFT_0400 0.44 +0.0 406,554 - B158DRAFT_0400 0.44 +0.0 406,559 - B158DRAFT_0400 0.45 +0.1 406,559 - B158DRAFT_0400 0.45 -0.0 406,576 - B158DRAFT_0400 0.46 -0.1 406,593 + B158DRAFT_0400 0.48 -0.8 406,642 + B158DRAFT_0400 0.52 -0.4 406,677 - B158DRAFT_0400 0.56 -0.2 406,682 - B158DRAFT_0400 0.56 -0.3 406,682 - B158DRAFT_0400 0.56 -0.4 406,735 - B158DRAFT_0400 0.61 -0.5 406,757 - B158DRAFT_0400 0.63 -0.9 406,806 + B158DRAFT_0400 0.67 -0.1 406,808 + B158DRAFT_0400 0.68 -0.9 406,808 + B158DRAFT_0400 0.68 -0.2 406,808 + B158DRAFT_0400 0.68 -0.2 406,816 - B158DRAFT_0400 0.68 -0.2 406,816 - B158DRAFT_0400 0.68 -0.8 406,816 - B158DRAFT_0400 0.68 -0.4 406,832 + B158DRAFT_0400 0.70 +0.5 406,851 + B158DRAFT_0400 0.72 -1.7 406,851 + B158DRAFT_0400 0.72 +1.2 406,851 + B158DRAFT_0400 0.72 -1.4 406,851 + B158DRAFT_0400 0.72 +0.1 406,859 - B158DRAFT_0400 0.72 -0.4 406,859 - B158DRAFT_0400 0.72 -2.7 406,859 - B158DRAFT_0400 0.72 -0.5 406,859 - B158DRAFT_0400 0.72 +0.9 406,859 - B158DRAFT_0400 0.72 -0.3 406,859 - B158DRAFT_0400 0.72 -0.5 406,859 - B158DRAFT_0400 0.72 -0.9 406,859 - B158DRAFT_0400 0.72 +0.2 406,859 - B158DRAFT_0400 0.72 -0.3 406,859 - B158DRAFT_0400 0.72 +0.0 406,859 - B158DRAFT_0400 0.72 +0.3 406,859 - B158DRAFT_0400 0.72 -0.3 406,859 - B158DRAFT_0400 0.72 -0.2 407,048 + B158DRAFT_0400 0.90 -0.1 407,048 + B158DRAFT_0400 0.90 -0.1 407,056 - +0.3 407,056 - -0.4 407,056 - +1.0 407,056 - +0.0 407,056 - +0.5 407,056 - -0.3 407,066 - -0.0 407,087 + -0.5 407,215 + -0.7 407,223 - -0.2 407,223 - +0.1 407,229 + -0.2 407,229 + -0.7 407,229 + -0.1 407,229 + -0.6 407,229 + -0.3 407,229 + -0.1 407,237 - -0.0 407,237 - +0.0 407,237 - +0.6 407,237 - +0.4 407,237 - -0.4 407,237 - -0.0 407,237 - +0.7 407,255 + +0.3 407,418 + B158DRAFT_0401 0.36 -1.2 407,453 + B158DRAFT_0401 0.43 -1.0 407,477 + B158DRAFT_0401 0.48 -0.5 407,504 + B158DRAFT_0401 0.54 -0.4 407,510 + B158DRAFT_0401 0.55 -0.7 407,511 + B158DRAFT_0401 0.55 -0.5 407,523 + B158DRAFT_0401 0.58 -0.2 407,523 + B158DRAFT_0401 0.58 +0.1 407,523 + B158DRAFT_0401 0.58 -0.2 407,523 + B158DRAFT_0401 0.58 -0.0 407,523 + B158DRAFT_0401 0.58 +0.0 407,523 + B158DRAFT_0401 0.58 -0.2 407,523 + B158DRAFT_0401 0.58 +0.2 407,526 - B158DRAFT_0401 0.58 +0.1 407,531 - B158DRAFT_0401 0.59 -0.2 407,531 - B158DRAFT_0401 0.59 +0.0 407,531 - B158DRAFT_0401 0.59 -0.5 407,531 - B158DRAFT_0401 0.59 +0.3 407,531 - B158DRAFT_0401 0.59 -0.4 407,712 - -0.4 407,742 + +0.1 407,745 - -0.0 407,755 + -0.1 407,755 + +0.1 407,755 + -0.2 407,755 + -1.2 407,763 - +0.1 407,763 - +0.9 407,777 + -0.4 407,777 + -0.3 407,785 - -0.1 407,786 - +0.3 407,859 - -0.1 407,859 - +0.1 407,895 + -2.2 407,957 + -0.6 408,012 + +0.0 408,012 + -0.9 408,106 + B158DRAFT_0402 0.20 +0.2 408,106 + B158DRAFT_0402 0.20 +0.4 408,119 + B158DRAFT_0402 0.21 -0.4 408,119 + B158DRAFT_0402 0.21 -0.6 408,119 + B158DRAFT_0402 0.21 -0.6 408,127 - B158DRAFT_0402 0.22 -0.3 408,127 - B158DRAFT_0402 0.22 -0.4 408,127 - B158DRAFT_0402 0.22 -1.1 408,132 - B158DRAFT_0402 0.23 -0.0 408,132 - B158DRAFT_0402 0.23 +0.2 408,209 + B158DRAFT_0402 0.32 +0.3 408,219 + B158DRAFT_0402 0.33 -0.1 408,260 + B158DRAFT_0402 0.38 -0.3 408,268 - B158DRAFT_0402 0.39 -0.6 408,289 - B158DRAFT_0402 0.42 +0.3 408,299 - B158DRAFT_0402 0.43 -0.8 408,328 + B158DRAFT_0402 0.46 -0.2 408,328 + B158DRAFT_0402 0.46 -0.1 408,328 + B158DRAFT_0402 0.46 +0.1 408,328 + B158DRAFT_0402 0.46 -0.3 408,336 - B158DRAFT_0402 0.47 -0.5 408,336 - B158DRAFT_0402 0.47 -0.1 408,336 - B158DRAFT_0402 0.47 -0.1 408,338 + B158DRAFT_0402 0.47 -0.1 408,338 + B158DRAFT_0402 0.47 -0.8 408,346 - B158DRAFT_0402 0.48 -0.5 408,381 - B158DRAFT_0402 0.52 -0.5 408,399 + B158DRAFT_0402 0.55 -0.0 408,404 + B158DRAFT_0402 0.55 -0.7 408,407 - B158DRAFT_0402 0.56 -2.1 408,414 + B158DRAFT_0402 0.56 +0.1 408,422 - B158DRAFT_0402 0.57 +0.6 408,448 - B158DRAFT_0402 0.60 -0.3 408,530 + B158DRAFT_0402 0.70 +0.2 408,537 + B158DRAFT_0402 0.71 -0.2 408,583 + B158DRAFT_0402 0.76 +0.3 408,589 + B158DRAFT_0402 0.77 -0.2 408,591 - B158DRAFT_0402 0.77 +0.4 408,597 - B158DRAFT_0402 0.78 -0.0 408,611 + B158DRAFT_0402 0.80 -1.1 408,611 + B158DRAFT_0402 0.80 +0.2 408,619 - B158DRAFT_0402 0.81 +0.2 408,619 - B158DRAFT_0402 0.81 -0.3 408,619 - B158DRAFT_0402 0.81 -0.1
Or see this region's nucleotide sequence