Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhfN and frlC are separated by 49 nucleotides frlC and frlD overlap by 4 nucleotides frlD and yhfR are separated by 99 nucleotides
b3371: yhfN - putative transport protein (VIMSS), at 3,499,290 to 3,500,312
yhfN
b4474: frlC - predicted isomerase (NCBI), at 3,500,362 to 3,501,192
frlC
b3374: frlD - fructoselysine 6-kinase (NCBI), at 3,501,189 to 3,501,974
frlD
b3375: yhfR - putative transcriptional regulator (VIMSS), at 3,502,074 to 3,502,805
yhfR
Position (kb)
3500
3501
3502 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3499.395 kb on + strand, within yhfN at 3499.398 kb on + strand, within yhfN at 3499.398 kb on + strand, within yhfN at 3499.401 kb on - strand, within yhfN at 3499.462 kb on - strand, within yhfN at 3499.508 kb on + strand, within yhfN at 3499.508 kb on + strand, within yhfN at 3499.532 kb on + strand, within yhfN at 3499.645 kb on - strand, within yhfN at 3499.664 kb on + strand, within yhfN at 3499.675 kb on + strand, within yhfN at 3499.762 kb on - strand, within yhfN at 3499.762 kb on - strand, within yhfN at 3499.767 kb on + strand, within yhfN at 3499.767 kb on + strand, within yhfN at 3499.775 kb on - strand, within yhfN at 3499.899 kb on - strand, within yhfN at 3499.914 kb on - strand, within yhfN at 3499.929 kb on - strand, within yhfN at 3499.935 kb on - strand, within yhfN at 3499.950 kb on + strand, within yhfN at 3499.982 kb on + strand, within yhfN at 3500.052 kb on + strand, within yhfN at 3500.136 kb on - strand, within yhfN at 3500.192 kb on + strand, within yhfN at 3500.237 kb on - strand at 3500.237 kb on - strand at 3500.239 kb on + strand at 3500.245 kb on - strand at 3500.318 kb on + strand at 3500.318 kb on + strand at 3500.364 kb on + strand at 3500.400 kb on + strand at 3500.437 kb on + strand at 3500.445 kb on - strand, within frlC at 3500.476 kb on - strand, within frlC at 3500.548 kb on + strand, within frlC at 3500.548 kb on + strand, within frlC at 3500.569 kb on + strand, within frlC at 3500.588 kb on + strand, within frlC at 3500.591 kb on - strand, within frlC at 3500.595 kb on - strand, within frlC at 3500.605 kb on + strand, within frlC at 3500.605 kb on + strand, within frlC at 3500.625 kb on - strand, within frlC at 3500.625 kb on - strand, within frlC at 3500.649 kb on + strand, within frlC at 3500.680 kb on - strand, within frlC at 3500.711 kb on - strand, within frlC at 3500.718 kb on + strand, within frlC at 3500.718 kb on + strand, within frlC at 3500.726 kb on - strand, within frlC at 3500.739 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.763 kb on + strand, within frlC at 3500.767 kb on - strand, within frlC at 3500.789 kb on + strand, within frlC at 3500.815 kb on - strand, within frlC at 3500.830 kb on + strand, within frlC at 3500.833 kb on + strand, within frlC at 3500.861 kb on + strand, within frlC at 3500.861 kb on - strand, within frlC at 3500.861 kb on - strand, within frlC at 3500.899 kb on + strand, within frlC at 3501.008 kb on - strand, within frlC at 3501.020 kb on - strand, within frlC at 3501.028 kb on - strand, within frlC at 3501.035 kb on + strand, within frlC at 3501.035 kb on + strand, within frlC at 3501.062 kb on + strand, within frlC at 3501.062 kb on + strand, within frlC at 3501.090 kb on + strand, within frlC at 3501.098 kb on - strand, within frlC at 3501.100 kb on - strand, within frlC at 3501.105 kb on + strand, within frlC at 3501.114 kb on + strand at 3501.114 kb on + strand at 3501.114 kb on + strand at 3501.123 kb on - strand at 3501.123 kb on - strand at 3501.125 kb on + strand at 3501.169 kb on + strand at 3501.169 kb on + strand at 3501.178 kb on + strand at 3501.239 kb on + strand at 3501.240 kb on - strand at 3501.284 kb on + strand, within frlD at 3501.292 kb on - strand, within frlD at 3501.330 kb on + strand, within frlD at 3501.382 kb on - strand, within frlD at 3501.410 kb on + strand, within frlD at 3501.498 kb on - strand, within frlD at 3501.498 kb on - strand, within frlD at 3501.517 kb on - strand, within frlD at 3501.522 kb on - strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.530 kb on + strand, within frlD at 3501.536 kb on - strand, within frlD at 3501.536 kb on - strand, within frlD at 3501.536 kb on - strand, within frlD at 3501.538 kb on - strand, within frlD at 3501.548 kb on - strand, within frlD at 3501.560 kb on - strand, within frlD at 3501.801 kb on + strand, within frlD at 3501.803 kb on + strand, within frlD at 3501.962 kb on - strand at 3501.964 kb on - strand at 3501.975 kb on + strand at 3501.975 kb on + strand at 3501.980 kb on + strand at 3501.998 kb on - strand at 3501.998 kb on - strand at 3502.109 kb on + strand at 3502.115 kb on - strand at 3502.147 kb on + strand at 3502.157 kb on + strand, within yhfR at 3502.161 kb on - strand, within yhfR at 3502.175 kb on - strand, within yhfR
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,499,395 + yhfN b3371 0.10 +0.9 3,499,398 + yhfN b3371 0.11 +2.0 3,499,398 + yhfN b3371 0.11 -1.0 3,499,401 - yhfN b3371 0.11 +0.6 3,499,462 - yhfN b3371 0.17 -0.2 3,499,508 + yhfN b3371 0.21 -2.0 3,499,508 + yhfN b3371 0.21 -2.4 3,499,532 + yhfN b3371 0.24 -1.4 3,499,645 - yhfN b3371 0.35 -0.7 3,499,664 + yhfN b3371 0.37 -0.3 3,499,675 + yhfN b3371 0.38 +0.3 3,499,762 - yhfN b3371 0.46 -0.0 3,499,762 - yhfN b3371 0.46 +0.2 3,499,767 + yhfN b3371 0.47 +0.1 3,499,767 + yhfN b3371 0.47 -0.4 3,499,775 - yhfN b3371 0.47 +0.4 3,499,899 - yhfN b3371 0.60 +0.0 3,499,914 - yhfN b3371 0.61 -0.6 3,499,929 - yhfN b3371 0.62 -0.7 3,499,935 - yhfN b3371 0.63 +0.4 3,499,950 + yhfN b3371 0.65 -0.7 3,499,982 + yhfN b3371 0.68 -0.6 3,500,052 + yhfN b3371 0.74 +0.3 3,500,136 - yhfN b3371 0.83 +0.2 3,500,192 + yhfN b3371 0.88 -0.2 3,500,237 - -0.6 3,500,237 - -0.6 3,500,239 + +0.4 3,500,245 - -0.1 3,500,318 + +0.6 3,500,318 + -0.3 3,500,364 + +0.3 3,500,400 + +0.3 3,500,437 + +0.9 3,500,445 - frlC b4474 0.10 +0.3 3,500,476 - frlC b4474 0.14 +0.5 3,500,548 + frlC b4474 0.22 -0.3 3,500,548 + frlC b4474 0.22 +0.7 3,500,569 + frlC b4474 0.25 -0.0 3,500,588 + frlC b4474 0.27 +0.7 3,500,591 - frlC b4474 0.28 -1.6 3,500,595 - frlC b4474 0.28 +0.2 3,500,605 + frlC b4474 0.29 -0.0 3,500,605 + frlC b4474 0.29 +0.8 3,500,625 - frlC b4474 0.32 -0.6 3,500,625 - frlC b4474 0.32 -0.6 3,500,649 + frlC b4474 0.35 -0.6 3,500,680 - frlC b4474 0.38 -1.0 3,500,711 - frlC b4474 0.42 -0.3 3,500,718 + frlC b4474 0.43 -0.6 3,500,718 + frlC b4474 0.43 -0.1 3,500,726 - frlC b4474 0.44 -0.3 3,500,739 + frlC b4474 0.45 -0.6 3,500,763 + frlC b4474 0.48 -0.4 3,500,763 + frlC b4474 0.48 -0.5 3,500,763 + frlC b4474 0.48 -0.0 3,500,763 + frlC b4474 0.48 -0.6 3,500,767 - frlC b4474 0.49 +0.1 3,500,789 + frlC b4474 0.51 +0.1 3,500,815 - frlC b4474 0.55 -0.1 3,500,830 + frlC b4474 0.56 +0.6 3,500,833 + frlC b4474 0.57 +0.4 3,500,861 + frlC b4474 0.60 +0.7 3,500,861 - frlC b4474 0.60 +0.8 3,500,861 - frlC b4474 0.60 +0.3 3,500,899 + frlC b4474 0.65 +0.6 3,501,008 - frlC b4474 0.78 +0.3 3,501,020 - frlC b4474 0.79 +0.0 3,501,028 - frlC b4474 0.80 -0.1 3,501,035 + frlC b4474 0.81 -0.9 3,501,035 + frlC b4474 0.81 +0.1 3,501,062 + frlC b4474 0.84 -0.7 3,501,062 + frlC b4474 0.84 +0.1 3,501,090 + frlC b4474 0.88 +1.4 3,501,098 - frlC b4474 0.89 -0.4 3,501,100 - frlC b4474 0.89 +2.1 3,501,105 + frlC b4474 0.89 -0.2 3,501,114 + +0.2 3,501,114 + +0.6 3,501,114 + +2.2 3,501,123 - -1.0 3,501,123 - +0.7 3,501,125 + -0.3 3,501,169 + +0.4 3,501,169 + -0.4 3,501,178 + -0.4 3,501,239 + +0.9 3,501,240 - -0.8 3,501,284 + frlD b3374 0.12 +0.4 3,501,292 - frlD b3374 0.13 -1.1 3,501,330 + frlD b3374 0.18 +0.8 3,501,382 - frlD b3374 0.25 -0.8 3,501,410 + frlD b3374 0.28 -0.3 3,501,498 - frlD b3374 0.39 -0.4 3,501,498 - frlD b3374 0.39 +0.0 3,501,517 - frlD b3374 0.42 -0.5 3,501,522 - frlD b3374 0.42 +0.5 3,501,530 + frlD b3374 0.43 +0.7 3,501,530 + frlD b3374 0.43 +0.3 3,501,530 + frlD b3374 0.43 +0.8 3,501,530 + frlD b3374 0.43 -0.3 3,501,530 + frlD b3374 0.43 +0.3 3,501,536 - frlD b3374 0.44 +0.0 3,501,536 - frlD b3374 0.44 -0.2 3,501,536 - frlD b3374 0.44 +0.5 3,501,538 - frlD b3374 0.44 -0.2 3,501,548 - frlD b3374 0.46 -0.8 3,501,560 - frlD b3374 0.47 +0.5 3,501,801 + frlD b3374 0.78 +0.5 3,501,803 + frlD b3374 0.78 -1.5 3,501,962 - +0.3 3,501,964 - -1.8 3,501,975 + +0.1 3,501,975 + +0.5 3,501,980 + -0.3 3,501,998 - +1.1 3,501,998 - -1.4 3,502,109 + -1.0 3,502,115 - -1.1 3,502,147 + -0.6 3,502,157 + yhfR b3375 0.11 -0.2 3,502,161 - yhfR b3375 0.12 -0.0 3,502,175 - yhfR b3375 0.14 +0.8
Or see this region's nucleotide sequence