Strain Fitness in Escherichia coli BW25113 around b2542

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthcaC and hcaB overlap by 4 nucleotideshcaB and hcaD are separated by 9 nucleotideshcaD and yphA are separated by 96 nucleotidesyphA and yphB are separated by 47 nucleotides b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250 hcaC b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059 hcaB b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271 hcaD b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790 yphA b2544: yphB - hypothetical protein (NCBI), at 2,671,838 to 2,672,710 yphB Position (kb) 2670 2671 2672Strain fitness (log2 ratio) -2 -1 0 1at 2669.083 kb on + strand, within hcaCat 2669.112 kb on - strand, within hcaCat 2669.166 kb on + strand, within hcaCat 2669.295 kb on + strandat 2669.442 kb on - strand, within hcaBat 2669.469 kb on - strandat 2669.662 kb on + strand, within hcaBat 2669.678 kb on - strand, within hcaBat 2669.678 kb on - strand, within hcaBat 2669.716 kb on - strand, within hcaBat 2669.716 kb on - strand, within hcaBat 2669.827 kb on + strand, within hcaBat 2669.827 kb on + strand, within hcaBat 2669.835 kb on - strand, within hcaBat 2669.835 kb on - strand, within hcaBat 2669.946 kb on + strand, within hcaBat 2669.947 kb on - strand, within hcaBat 2669.951 kb on + strand, within hcaBat 2669.951 kb on + strand, within hcaBat 2670.153 kb on + strandat 2670.199 kb on + strand, within hcaDat 2670.427 kb on - strand, within hcaDat 2670.427 kb on - strand, within hcaDat 2670.447 kb on + strand, within hcaDat 2670.450 kb on - strand, within hcaDat 2670.450 kb on - strand, within hcaDat 2670.576 kb on - strand, within hcaDat 2670.576 kb on - strand, within hcaDat 2670.576 kb on - strand, within hcaDat 2670.603 kb on + strand, within hcaDat 2670.836 kb on - strand, within hcaDat 2670.927 kb on - strand, within hcaDat 2670.994 kb on - strand, within hcaDat 2670.994 kb on - strand, within hcaDat 2671.037 kb on + strand, within hcaDat 2671.203 kb on + strandat 2671.315 kb on + strandat 2671.315 kb on + strandat 2671.315 kb on + strandat 2671.323 kb on - strandat 2671.373 kb on - strandat 2671.373 kb on - strandat 2671.494 kb on + strand, within yphAat 2671.494 kb on + strand, within yphAat 2671.496 kb on + strand, within yphAat 2671.497 kb on - strand, within yphAat 2671.502 kb on - strand, within yphAat 2671.502 kb on - strand, within yphAat 2671.509 kb on + strand, within yphAat 2671.603 kb on + strand, within yphAat 2671.641 kb on - strand, within yphAat 2671.645 kb on - strand, within yphAat 2671.713 kb on + strand, within yphAat 2671.736 kb on + strand, within yphAat 2671.736 kb on + strand, within yphAat 2671.789 kb on + strandat 2671.890 kb on - strandat 2671.959 kb on - strand, within yphBat 2672.031 kb on - strand, within yphBat 2672.031 kb on - strand, within yphBat 2672.053 kb on + strand, within yphBat 2672.081 kb on + strand, within yphBat 2672.084 kb on - strand, within yphBat 2672.165 kb on - strand, within yphBat 2672.165 kb on - strand, within yphB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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2,669,083 + hcaC b2540 0.48 -0.2
2,669,112 - hcaC b2540 0.57 -0.3
2,669,166 + hcaC b2540 0.74 +0.8
2,669,295 + +0.1
2,669,442 - hcaB b2541 0.24 -1.3
2,669,469 - +1.5
2,669,662 + hcaB b2541 0.51 +0.1
2,669,678 - hcaB b2541 0.53 +0.1
2,669,678 - hcaB b2541 0.53 -0.5
2,669,716 - hcaB b2541 0.58 -0.2
2,669,716 - hcaB b2541 0.58 +0.8
2,669,827 + hcaB b2541 0.71 -1.9
2,669,827 + hcaB b2541 0.71 +0.4
2,669,835 - hcaB b2541 0.72 +1.6
2,669,835 - hcaB b2541 0.72 +0.6
2,669,946 + hcaB b2541 0.86 +1.4
2,669,947 - hcaB b2541 0.86 +0.2
2,669,951 + hcaB b2541 0.87 +0.4
2,669,951 + hcaB b2541 0.87 -1.5
2,670,153 + +0.0
2,670,199 + hcaD b2542 0.11 +0.1
2,670,427 - hcaD b2542 0.30 +0.3
2,670,427 - hcaD b2542 0.30 -0.7
2,670,447 + hcaD b2542 0.31 +0.3
2,670,450 - hcaD b2542 0.32 +1.5
2,670,450 - hcaD b2542 0.32 -1.1
2,670,576 - hcaD b2542 0.42 +0.6
2,670,576 - hcaD b2542 0.42 +0.3
2,670,576 - hcaD b2542 0.42 -0.5
2,670,603 + hcaD b2542 0.44 +0.4
2,670,836 - hcaD b2542 0.64 -0.3
2,670,927 - hcaD b2542 0.71 +0.0
2,670,994 - hcaD b2542 0.77 -0.3
2,670,994 - hcaD b2542 0.77 +1.4
2,671,037 + hcaD b2542 0.80 -0.5
2,671,203 + +1.1
2,671,315 + +0.5
2,671,315 + +0.1
2,671,315 + +0.4
2,671,323 - +0.6
2,671,373 - +0.2
2,671,373 - -0.3
2,671,494 + yphA b2543 0.30 -0.2
2,671,494 + yphA b2543 0.30 +0.3
2,671,496 + yphA b2543 0.30 +0.3
2,671,497 - yphA b2543 0.30 +0.1
2,671,502 - yphA b2543 0.32 +0.3
2,671,502 - yphA b2543 0.32 +0.9
2,671,509 + yphA b2543 0.33 +0.9
2,671,603 + yphA b2543 0.56 -0.2
2,671,641 - yphA b2543 0.65 -0.3
2,671,645 - yphA b2543 0.65 +1.1
2,671,713 + yphA b2543 0.82 +0.5
2,671,736 + yphA b2543 0.87 -0.2
2,671,736 + yphA b2543 0.87 +1.0
2,671,789 + +0.7
2,671,890 - -0.1
2,671,959 - yphB b2544 0.14 -0.7
2,672,031 - yphB b2544 0.22 +1.2
2,672,031 - yphB b2544 0.22 -0.2
2,672,053 + yphB b2544 0.25 -0.0
2,672,081 + yphB b2544 0.28 +0.1
2,672,084 - yphB b2544 0.28 +0.1
2,672,165 - yphB b2544 0.37 +0.7
2,672,165 - yphB b2544 0.37 -0.2

Or see this region's nucleotide sequence