Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hofB and ppdD are separated by 9 nucleotides ppdD and nadC are separated by 202 nucleotides nadC and ampD are separated by 87 nucleotides ampD and ampE overlap by 4 nucleotides
b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099
hofB
b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549
ppdD
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 116.760 kb on - strand, within hofB at 116.777 kb on + strand, within hofB at 116.777 kb on + strand, within hofB at 116.872 kb on + strand, within hofB at 116.882 kb on + strand, within hofB at 116.957 kb on - strand, within hofB at 116.964 kb on - strand at 117.046 kb on + strand at 117.056 kb on + strand at 117.069 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.081 kb on - strand at 117.081 kb on - strand at 117.084 kb on + strand at 117.087 kb on + strand at 117.227 kb on - strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.293 kb on - strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.385 kb on + strand, within ppdD at 117.387 kb on + strand, within ppdD at 117.508 kb on - strand at 117.508 kb on - strand at 117.511 kb on - strand at 117.511 kb on - strand at 117.525 kb on - strand at 117.525 kb on - strand at 117.603 kb on + strand at 117.603 kb on + strand at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 118.107 kb on - strand, within nadC at 118.201 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.296 kb on + strand, within nadC at 118.355 kb on - strand, within nadC at 118.355 kb on - strand, within nadC at 118.364 kb on + strand, within nadC at 118.382 kb on - strand, within nadC at 118.538 kb on + strand, within nadC at 118.623 kb on + strand at 118.631 kb on - strand at 118.737 kb on + strand at 118.737 kb on + strand at 118.781 kb on + strand at 118.836 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.904 kb on - strand, within ampD at 119.033 kb on - strand, within ampD at 119.101 kb on + strand, within ampD at 119.109 kb on - strand, within ampD at 119.109 kb on - strand, within ampD at 119.117 kb on - strand, within ampD at 119.119 kb on + strand, within ampD at 119.150 kb on - strand, within ampD at 119.150 kb on - strand, within ampD at 119.163 kb on + strand, within ampD at 119.168 kb on - strand, within ampD at 119.191 kb on + strand, within ampD at 119.191 kb on + strand, within ampD at 119.274 kb on + strand at 119.339 kb on + strand at 119.339 kb on + strand at 119.499 kb on - strand, within ampE at 119.499 kb on - strand, within ampE at 119.522 kb on + strand, within ampE at 119.528 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.558 kb on - strand, within ampE at 119.567 kb on + strand, within ampE at 119.567 kb on + strand, within ampE
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 116,760 - hofB b0107 0.75 +0.1 116,777 + hofB b0107 0.77 -1.1 116,777 + hofB b0107 0.77 -0.2 116,872 + hofB b0107 0.84 -0.1 116,882 + hofB b0107 0.84 +0.1 116,957 - hofB b0107 0.90 +0.9 116,964 - -0.5 117,046 + -0.2 117,056 + +0.0 117,069 + -0.1 117,075 + +1.6 117,075 + -1.8 117,075 + +0.2 117,081 - +0.4 117,081 - -0.6 117,084 + +0.3 117,087 + -1.1 117,227 - ppdD b0108 0.27 +0.3 117,281 + ppdD b0108 0.39 -0.7 117,281 + ppdD b0108 0.39 +0.2 117,293 - ppdD b0108 0.42 +0.2 117,302 + ppdD b0108 0.44 +0.3 117,302 + ppdD b0108 0.44 -0.6 117,385 + ppdD b0108 0.63 -0.8 117,387 + ppdD b0108 0.63 -0.2 117,508 - -0.4 117,508 - +0.9 117,511 - -0.3 117,511 - -0.0 117,525 - +0.6 117,525 - -0.4 117,603 + +1.2 117,603 + +0.2 117,839 + +1.1 117,856 - nadC b0109 0.12 -0.9 117,856 - nadC b0109 0.12 +3.6 117,879 - nadC b0109 0.14 -1.8 117,879 - nadC b0109 0.14 -0.5 117,881 - nadC b0109 0.14 +0.4 117,922 - nadC b0109 0.19 +0.1 117,922 - nadC b0109 0.19 +1.1 118,107 - nadC b0109 0.40 -0.2 118,201 + nadC b0109 0.50 +0.2 118,230 + nadC b0109 0.53 +1.1 118,230 + nadC b0109 0.53 -0.4 118,296 + nadC b0109 0.61 -0.4 118,355 - nadC b0109 0.67 -0.1 118,355 - nadC b0109 0.67 +1.3 118,364 + nadC b0109 0.68 +0.3 118,382 - nadC b0109 0.70 -1.5 118,538 + nadC b0109 0.88 -0.2 118,623 + -0.1 118,631 - -0.6 118,737 + -0.1 118,737 + -0.5 118,781 + +0.2 118,836 + ampD b0110 0.19 -0.6 118,868 + ampD b0110 0.24 -0.4 118,868 + ampD b0110 0.24 -1.1 118,904 - ampD b0110 0.31 +0.0 119,033 - ampD b0110 0.54 +0.3 119,101 + ampD b0110 0.67 +0.2 119,109 - ampD b0110 0.68 +0.0 119,109 - ampD b0110 0.68 -0.3 119,117 - ampD b0110 0.70 +1.1 119,119 + ampD b0110 0.70 -0.6 119,150 - ampD b0110 0.76 +0.6 119,150 - ampD b0110 0.76 -0.6 119,163 + ampD b0110 0.78 +0.9 119,168 - ampD b0110 0.79 +0.1 119,191 + ampD b0110 0.83 +0.2 119,191 + ampD b0110 0.83 -0.9 119,274 + -0.4 119,339 + +0.8 119,339 + +0.5 119,499 - ampE b0111 0.25 -0.2 119,499 - ampE b0111 0.25 -0.1 119,522 + ampE b0111 0.28 -0.9 119,528 - ampE b0111 0.29 -1.0 119,555 - ampE b0111 0.32 -0.9 119,555 - ampE b0111 0.32 -0.4 119,558 - ampE b0111 0.32 +0.1 119,567 + ampE b0111 0.33 +0.8 119,567 + ampE b0111 0.33 +0.2
Or see this region's nucleotide sequence