Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF990 and GFF991 are separated by 56 nucleotides GFF991 and GFF992 are separated by 69 nucleotides GFF992 and GFF993 are separated by 71 nucleotides GFF993 and GFF994 overlap by 4 nucleotides GFF994 and GFF995 are separated by 70 nucleotides
GFF990 - FIG00637933: hypothetical protein, at 175,394 to 176,635
GFF990
GFF991 - hypothetical protein, at 176,692 to 176,835
GFF991
GFF992 - hypothetical protein, at 176,905 to 177,042
GFF992
GFF993 - Transposase STY4875, at 177,114 to 177,629
GFF993
GFF994 - hypothetical protein, at 177,626 to 177,739
GFF994
GFF995 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 177,810 to 179,180
GFF995
Position (kb) 176
177
178 Strain fitness (log2 ratio) -2
-1
0
1
2 at 175.944 kb on + strand, within GFF990 at 176.203 kb on + strand, within GFF990 at 176.204 kb on - strand, within GFF990 at 176.515 kb on + strand at 176.751 kb on - strand, within GFF991 at 176.831 kb on - strand at 176.878 kb on - strand at 176.909 kb on + strand at 176.934 kb on + strand, within GFF992 at 176.934 kb on + strand, within GFF992 at 176.934 kb on + strand, within GFF992 at 177.076 kb on + strand at 177.076 kb on + strand at 177.271 kb on + strand, within GFF993 at 177.272 kb on - strand, within GFF993 at 177.276 kb on - strand, within GFF993 at 177.314 kb on + strand, within GFF993 at 177.318 kb on + strand, within GFF993 at 177.318 kb on + strand, within GFF993 at 177.324 kb on - strand, within GFF993 at 177.395 kb on + strand, within GFF993 at 177.419 kb on - strand, within GFF993 at 177.684 kb on + strand, within GFF994 at 177.685 kb on - strand, within GFF994 at 177.689 kb on - strand, within GFF994 at 177.744 kb on - strand at 177.753 kb on - strand at 177.772 kb on - strand at 177.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 175,944 + GFF990 0.44 -0.1 176,203 + GFF990 0.65 +1.0 176,204 - GFF990 0.65 -0.2 176,515 + -0.2 176,751 - GFF991 0.41 -0.6 176,831 - +0.1 176,878 - -0.2 176,909 + -0.7 176,934 + GFF992 0.21 -0.6 176,934 + GFF992 0.21 -1.2 176,934 + GFF992 0.21 -0.6 177,076 + -0.8 177,076 + +1.1 177,271 + GFF993 0.30 -1.9 177,272 - GFF993 0.31 +2.3 177,276 - GFF993 0.31 +0.5 177,314 + GFF993 0.39 -0.1 177,318 + GFF993 0.40 -0.7 177,318 + GFF993 0.40 -0.8 177,324 - GFF993 0.41 -0.2 177,395 + GFF993 0.54 +0.5 177,419 - GFF993 0.59 -0.6 177,684 + GFF994 0.51 -0.6 177,685 - GFF994 0.52 -0.7 177,689 - GFF994 0.55 +0.4 177,744 - -0.7 177,753 - +1.2 177,772 - -0.7 177,772 - -0.2
Or see this region's nucleotide sequence