Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF821

Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF820 and GFF821 overlap by 26 nucleotidesGFF821 and GFF822 are separated by 16 nucleotides GFF820 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 1,720 to 3,264 GFF820 GFF821 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 3,239 to 3,697 GFF821 GFF822 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 3,714 to 5,033 GFF822 Position (kb) 3 4Strain fitness (log2 ratio) -1 0 1 2at 2.348 kb on + strand, within GFF820at 2.467 kb on - strand, within GFF820at 2.576 kb on + strand, within GFF820at 2.633 kb on + strand, within GFF820at 2.634 kb on - strand, within GFF820at 2.763 kb on + strand, within GFF820at 2.763 kb on + strand, within GFF820at 2.763 kb on + strand, within GFF820at 2.763 kb on + strand, within GFF820at 2.901 kb on - strand, within GFF820at 2.922 kb on - strand, within GFF820at 3.230 kb on + strandat 3.699 kb on + strandat 3.699 kb on + strandat 3.700 kb on - strandat 3.742 kb on + strandat 3.751 kb on + strandat 3.752 kb on - strandat 3.830 kb on + strandat 3.865 kb on - strand, within GFF822at 4.068 kb on + strand, within GFF822at 4.069 kb on - strand, within GFF822at 4.069 kb on - strand, within GFF822at 4.372 kb on + strand, within GFF822at 4.382 kb on - strand, within GFF822at 4.382 kb on - strand, within GFF822at 4.389 kb on - strand, within GFF822at 4.403 kb on + strand, within GFF822at 4.621 kb on + strand, within GFF822at 4.666 kb on - strand, within GFF822

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
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2,348 + GFF820 0.41 -0.1
2,467 - GFF820 0.48 +1.1
2,576 + GFF820 0.55 -0.6
2,633 + GFF820 0.59 -0.2
2,634 - GFF820 0.59 +1.9
2,763 + GFF820 0.68 +0.9
2,763 + GFF820 0.68 +1.0
2,763 + GFF820 0.68 -0.6
2,763 + GFF820 0.68 -0.8
2,901 - GFF820 0.76 +0.1
2,922 - GFF820 0.78 -0.2
3,230 + -0.0
3,699 + -0.4
3,699 + -0.5
3,700 - +0.3
3,742 + -1.6
3,751 + -0.2
3,752 - +2.5
3,830 + +0.8
3,865 - GFF822 0.11 +0.6
4,068 + GFF822 0.27 -0.3
4,069 - GFF822 0.27 +0.6
4,069 - GFF822 0.27 +2.7
4,372 + GFF822 0.50 +1.1
4,382 - GFF822 0.51 -0.0
4,382 - GFF822 0.51 -1.6
4,389 - GFF822 0.51 -0.5
4,403 + GFF822 0.52 -0.5
4,621 + GFF822 0.69 -0.5
4,666 - GFF822 0.72 -0.7

Or see this region's nucleotide sequence