Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF79 and GFF80 overlap by 1 nucleotides GFF80 and GFF81 are separated by 48 nucleotides GFF81 and GFF82 are separated by 91 nucleotides GFF82 and GFF83 are separated by 138 nucleotides
GFF79 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), periplasmic substrate binding protein FhuD, at 61,426 to 62,316
GFF79
GFF80 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), ATP-binding protein FhuC, at 62,316 to 63,113
GFF80
GFF81 - Ferric hydroxamate outer membrane receptor FhuA, at 63,162 to 65,273
GFF81
GFF82 - hypothetical protein, at 65,365 to 65,505
GFF82
GFF83 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 65,644 to 68,166
GFF83
Position (kb) 63
64
65
66 Strain fitness (log2 ratio) -1
0
1
2
3 at 62.281 kb on + strand at 62.295 kb on + strand at 62.304 kb on - strand at 62.317 kb on + strand at 62.318 kb on - strand at 62.318 kb on - strand at 62.320 kb on + strand at 62.320 kb on + strand at 62.381 kb on - strand at 62.464 kb on + strand, within GFF80 at 62.704 kb on - strand, within GFF80 at 62.730 kb on + strand, within GFF80 at 62.848 kb on + strand, within GFF80 at 62.966 kb on + strand, within GFF80 at 62.987 kb on + strand, within GFF80 at 62.988 kb on - strand, within GFF80 at 63.087 kb on - strand at 63.220 kb on + strand at 63.263 kb on + strand at 63.316 kb on + strand at 63.316 kb on + strand at 63.351 kb on + strand at 63.448 kb on + strand, within GFF81 at 63.536 kb on + strand, within GFF81 at 63.537 kb on - strand, within GFF81 at 63.537 kb on - strand, within GFF81 at 63.537 kb on - strand, within GFF81 at 63.538 kb on + strand, within GFF81 at 63.538 kb on + strand, within GFF81 at 63.539 kb on - strand, within GFF81 at 63.539 kb on - strand, within GFF81 at 63.539 kb on - strand, within GFF81 at 63.557 kb on + strand, within GFF81 at 63.558 kb on - strand, within GFF81 at 63.598 kb on - strand, within GFF81 at 63.746 kb on - strand, within GFF81 at 63.826 kb on + strand, within GFF81 at 63.826 kb on + strand, within GFF81 at 63.827 kb on - strand, within GFF81 at 63.835 kb on + strand, within GFF81 at 63.863 kb on - strand, within GFF81 at 63.900 kb on - strand, within GFF81 at 63.908 kb on + strand, within GFF81 at 63.909 kb on - strand, within GFF81 at 64.085 kb on - strand, within GFF81 at 64.097 kb on - strand, within GFF81 at 64.187 kb on + strand, within GFF81 at 64.230 kb on - strand, within GFF81 at 64.403 kb on - strand, within GFF81 at 64.429 kb on + strand, within GFF81 at 64.552 kb on + strand, within GFF81 at 64.566 kb on - strand, within GFF81 at 64.782 kb on - strand, within GFF81 at 64.997 kb on + strand, within GFF81 at 65.026 kb on - strand, within GFF81 at 65.132 kb on + strand at 65.133 kb on - strand at 65.296 kb on - strand at 65.355 kb on + strand at 65.356 kb on - strand at 65.357 kb on + strand at 65.396 kb on - strand, within GFF82 at 65.528 kb on + strand at 65.572 kb on + strand at 65.809 kb on - strand at 65.874 kb on - strand at 65.917 kb on + strand, within GFF83 at 65.982 kb on - strand, within GFF83 at 66.179 kb on + strand, within GFF83 at 66.228 kb on - strand, within GFF83 at 66.238 kb on - strand, within GFF83
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 62,281 + +0.7 62,295 + +0.3 62,304 - -0.2 62,317 + +3.5 62,318 - -0.1 62,318 - -0.7 62,320 + -0.4 62,320 + +0.3 62,381 - +1.5 62,464 + GFF80 0.19 -0.3 62,704 - GFF80 0.49 +0.3 62,730 + GFF80 0.52 -0.3 62,848 + GFF80 0.67 -0.9 62,966 + GFF80 0.81 +1.5 62,987 + GFF80 0.84 +0.5 62,988 - GFF80 0.84 -1.2 63,087 - -0.5 63,220 + -0.3 63,263 + +0.0 63,316 + +0.8 63,316 + +0.7 63,351 + +0.6 63,448 + GFF81 0.14 -0.9 63,536 + GFF81 0.18 +1.4 63,537 - GFF81 0.18 -0.2 63,537 - GFF81 0.18 +0.9 63,537 - GFF81 0.18 -1.5 63,538 + GFF81 0.18 +0.9 63,538 + GFF81 0.18 +0.9 63,539 - GFF81 0.18 +1.1 63,539 - GFF81 0.18 +1.0 63,539 - GFF81 0.18 +0.3 63,557 + GFF81 0.19 +0.6 63,558 - GFF81 0.19 +0.9 63,598 - GFF81 0.21 -0.0 63,746 - GFF81 0.28 +0.1 63,826 + GFF81 0.31 +0.6 63,826 + GFF81 0.31 +0.7 63,827 - GFF81 0.31 -0.3 63,835 + GFF81 0.32 -0.3 63,863 - GFF81 0.33 -0.2 63,900 - GFF81 0.35 -1.0 63,908 + GFF81 0.35 +0.8 63,909 - GFF81 0.35 -0.6 64,085 - GFF81 0.44 -1.1 64,097 - GFF81 0.44 -0.0 64,187 + GFF81 0.49 +0.6 64,230 - GFF81 0.51 +0.3 64,403 - GFF81 0.59 +0.2 64,429 + GFF81 0.60 -0.3 64,552 + GFF81 0.66 -0.4 64,566 - GFF81 0.66 +1.8 64,782 - GFF81 0.77 +1.8 64,997 + GFF81 0.87 +0.0 65,026 - GFF81 0.88 -0.9 65,132 + -0.6 65,133 - +1.4 65,296 - +0.2 65,355 + +0.4 65,356 - +0.2 65,357 + -0.1 65,396 - GFF82 0.22 +1.5 65,528 + -0.7 65,572 + -0.1 65,809 - +0.9 65,874 - +0.0 65,917 + GFF83 0.11 +0.0 65,982 - GFF83 0.13 +0.5 66,179 + GFF83 0.21 +0.2 66,228 - GFF83 0.23 -1.2 66,238 - GFF83 0.24 +0.1
Or see this region's nucleotide sequence