Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF758 and GFF759 are separated by 87 nucleotides GFF759 and GFF760 are separated by 9 nucleotides GFF760 and GFF761 overlap by 4 nucleotides
GFF758 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 115,446 to 116,561
GFF758
GFF759 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 116,649 to 117,782
GFF759
GFF760 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 117,792 to 118,745
GFF760
GFF761 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 118,742 to 119,587
GFF761
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 115.664 kb on - strand, within GFF758 at 115.833 kb on + strand, within GFF758 at 115.833 kb on + strand, within GFF758 at 115.833 kb on + strand, within GFF758 at 115.846 kb on - strand, within GFF758 at 115.847 kb on + strand, within GFF758 at 115.848 kb on - strand, within GFF758 at 115.874 kb on + strand, within GFF758 at 115.875 kb on - strand, within GFF758 at 116.099 kb on - strand, within GFF758 at 116.134 kb on - strand, within GFF758 at 116.337 kb on - strand, within GFF758 at 116.337 kb on - strand, within GFF758 at 116.389 kb on - strand, within GFF758 at 116.556 kb on - strand at 116.564 kb on + strand at 116.565 kb on - strand at 116.583 kb on + strand at 116.771 kb on + strand, within GFF759 at 116.775 kb on + strand, within GFF759 at 116.775 kb on + strand, within GFF759 at 116.776 kb on - strand, within GFF759 at 116.776 kb on - strand, within GFF759 at 116.930 kb on + strand, within GFF759 at 116.930 kb on + strand, within GFF759 at 117.084 kb on - strand, within GFF759 at 117.152 kb on + strand, within GFF759 at 117.152 kb on + strand, within GFF759 at 117.528 kb on + strand, within GFF759 at 117.528 kb on + strand, within GFF759 at 117.528 kb on + strand, within GFF759 at 117.529 kb on - strand, within GFF759 at 117.538 kb on + strand, within GFF759 at 117.615 kb on - strand, within GFF759 at 117.621 kb on + strand, within GFF759 at 117.638 kb on + strand, within GFF759 at 117.643 kb on - strand, within GFF759 at 117.778 kb on + strand at 117.778 kb on + strand at 117.780 kb on + strand at 117.901 kb on - strand, within GFF760 at 118.235 kb on - strand, within GFF760 at 118.354 kb on - strand, within GFF760 at 118.355 kb on + strand, within GFF760 at 118.369 kb on - strand, within GFF760 at 118.391 kb on - strand, within GFF760 at 118.401 kb on + strand, within GFF760 at 118.593 kb on - strand, within GFF760 at 118.668 kb on - strand at 118.670 kb on + strand at 118.670 kb on + strand at 118.671 kb on - strand at 118.671 kb on - strand at 118.687 kb on + strand at 118.688 kb on - strand at 118.710 kb on - strand at 118.747 kb on + strand at 118.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 115,664 - GFF758 0.20 -0.8 115,833 + GFF758 0.35 -0.0 115,833 + GFF758 0.35 +0.3 115,833 + GFF758 0.35 +1.2 115,846 - GFF758 0.36 +0.3 115,847 + GFF758 0.36 -0.1 115,848 - GFF758 0.36 +0.9 115,874 + GFF758 0.38 -0.1 115,875 - GFF758 0.38 -0.7 116,099 - GFF758 0.59 -0.2 116,134 - GFF758 0.62 -0.3 116,337 - GFF758 0.80 -0.3 116,337 - GFF758 0.80 +0.4 116,389 - GFF758 0.84 -0.5 116,556 - -0.0 116,564 + -0.8 116,565 - -0.4 116,583 + -0.8 116,771 + GFF759 0.11 +1.0 116,775 + GFF759 0.11 -0.5 116,775 + GFF759 0.11 -0.4 116,776 - GFF759 0.11 +0.4 116,776 - GFF759 0.11 +1.0 116,930 + GFF759 0.25 -0.1 116,930 + GFF759 0.25 -0.2 117,084 - GFF759 0.38 +0.1 117,152 + GFF759 0.44 -0.5 117,152 + GFF759 0.44 -0.2 117,528 + GFF759 0.78 -2.1 117,528 + GFF759 0.78 -0.3 117,528 + GFF759 0.78 +0.7 117,529 - GFF759 0.78 -0.3 117,538 + GFF759 0.78 -0.0 117,615 - GFF759 0.85 -0.2 117,621 + GFF759 0.86 +0.4 117,638 + GFF759 0.87 -0.7 117,643 - GFF759 0.88 -0.1 117,778 + -0.1 117,778 + -0.3 117,780 + -0.8 117,901 - GFF760 0.11 -0.1 118,235 - GFF760 0.46 -0.2 118,354 - GFF760 0.59 +1.4 118,355 + GFF760 0.59 +1.1 118,369 - GFF760 0.60 -0.4 118,391 - GFF760 0.63 -1.7 118,401 + GFF760 0.64 +1.8 118,593 - GFF760 0.84 +0.2 118,668 - +2.4 118,670 + -0.2 118,670 + -0.5 118,671 - -1.0 118,671 - -0.0 118,687 + -0.6 118,688 - +0.2 118,710 - +2.0 118,747 + -0.6 118,748 - -0.7
Or see this region's nucleotide sequence