Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF703 and GFF704 are separated by 118 nucleotides GFF704 and GFF705 overlap by 4 nucleotides GFF705 and GFF706 are separated by 143 nucleotides
GFF703 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 59,465 to 61,687
GFF703
GFF704 - Glutamate transport ATP-binding protein, at 61,806 to 62,528
GFF704
GFF705 - Glutamate transport membrane-spanning protein, at 62,525 to 63,184
GFF705
GFF706 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 63,328 to 64,074
GFF706
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 60.881 kb on - strand, within GFF703 at 61.064 kb on - strand, within GFF703 at 61.088 kb on + strand, within GFF703 at 61.209 kb on - strand, within GFF703 at 61.726 kb on - strand at 61.727 kb on + strand at 61.727 kb on + strand at 61.900 kb on + strand, within GFF704 at 62.384 kb on + strand, within GFF704 at 62.391 kb on - strand, within GFF704 at 62.391 kb on - strand, within GFF704 at 62.397 kb on + strand, within GFF704 at 62.401 kb on - strand, within GFF704 at 62.474 kb on + strand at 62.475 kb on - strand at 62.475 kb on - strand at 62.478 kb on + strand at 62.479 kb on - strand at 62.689 kb on + strand, within GFF705 at 62.714 kb on - strand, within GFF705 at 62.894 kb on - strand, within GFF705 at 63.184 kb on + strand at 63.212 kb on + strand at 63.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 60,881 - GFF703 0.64 +0.4 61,064 - GFF703 0.72 -0.5 61,088 + GFF703 0.73 +2.3 61,209 - GFF703 0.78 +0.6 61,726 - -0.0 61,727 + +4.8 61,727 + +0.1 61,900 + GFF704 0.13 -0.4 62,384 + GFF704 0.80 +0.2 62,391 - GFF704 0.81 -0.5 62,391 - GFF704 0.81 -0.3 62,397 + GFF704 0.82 -0.5 62,401 - GFF704 0.82 +0.4 62,474 + +1.0 62,475 - -0.7 62,475 - -0.3 62,478 + +1.2 62,479 - -0.7 62,689 + GFF705 0.25 +0.8 62,714 - GFF705 0.29 +0.0 62,894 - GFF705 0.56 -0.2 63,184 + -0.2 63,212 + -0.2 63,371 + -0.2
Or see this region's nucleotide sequence