Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF662 and GFF663 are separated by 237 nucleotides GFF663 and GFF664 overlap by 4 nucleotides GFF664 and GFF665 are separated by 44 nucleotides
GFF662 - Pectinesterase (EC 3.1.1.11), at 17,384 to 18,667
GFF662
GFF663 - Imidazolonepropionase (EC 3.5.2.7), at 18,905 to 20,128
GFF663
GFF664 - Formiminoglutamase (EC 3.5.3.8), at 20,125 to 21,066
GFF664
GFF665 - Histidine utilization repressor, at 21,111 to 21,836
GFF665
Position (kb) 18
19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 18.084 kb on - strand, within GFF662 at 18.127 kb on + strand, within GFF662 at 18.127 kb on + strand, within GFF662 at 18.129 kb on + strand, within GFF662 at 18.130 kb on - strand, within GFF662 at 18.151 kb on + strand, within GFF662 at 18.152 kb on - strand, within GFF662 at 18.153 kb on + strand, within GFF662 at 18.154 kb on - strand, within GFF662 at 18.289 kb on - strand, within GFF662 at 18.303 kb on + strand, within GFF662 at 18.320 kb on - strand, within GFF662 at 18.320 kb on - strand, within GFF662 at 18.328 kb on - strand, within GFF662 at 18.403 kb on + strand, within GFF662 at 18.602 kb on - strand at 18.602 kb on - strand at 18.602 kb on - strand at 18.661 kb on + strand at 18.662 kb on - strand at 18.726 kb on + strand at 18.726 kb on + strand at 18.726 kb on + strand at 18.804 kb on + strand at 18.863 kb on + strand at 18.871 kb on + strand at 18.872 kb on - strand at 18.873 kb on + strand at 18.997 kb on + strand at 19.027 kb on + strand at 19.444 kb on + strand, within GFF663 at 19.478 kb on + strand, within GFF663 at 19.614 kb on + strand, within GFF663 at 19.713 kb on - strand, within GFF663 at 20.067 kb on + strand at 20.068 kb on - strand at 20.068 kb on - strand at 20.068 kb on - strand at 20.072 kb on + strand at 20.073 kb on - strand at 20.073 kb on - strand at 20.094 kb on + strand at 20.178 kb on + strand at 20.179 kb on - strand at 20.180 kb on + strand at 20.180 kb on + strand at 20.181 kb on - strand at 20.181 kb on - strand at 20.581 kb on - strand, within GFF664 at 20.842 kb on - strand, within GFF664 at 20.855 kb on + strand, within GFF664 at 20.855 kb on + strand, within GFF664 at 21.114 kb on + strand at 21.114 kb on + strand at 21.115 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 18,084 - GFF662 0.55 -2.5 18,127 + GFF662 0.58 +0.9 18,127 + GFF662 0.58 -1.7 18,129 + GFF662 0.58 +1.5 18,130 - GFF662 0.58 -0.4 18,151 + GFF662 0.60 +0.7 18,152 - GFF662 0.60 +2.8 18,153 + GFF662 0.60 +2.1 18,154 - GFF662 0.60 -0.4 18,289 - GFF662 0.70 +1.2 18,303 + GFF662 0.72 +1.6 18,320 - GFF662 0.73 -0.2 18,320 - GFF662 0.73 -0.4 18,328 - GFF662 0.74 -0.2 18,403 + GFF662 0.79 -0.7 18,602 - +2.8 18,602 - -0.5 18,602 - +0.9 18,661 + +0.3 18,662 - -0.5 18,726 + -0.2 18,726 + -0.3 18,726 + -0.1 18,804 + -0.5 18,863 + +0.5 18,871 + +0.3 18,872 - -1.1 18,873 + +0.2 18,997 + +0.6 19,027 + -0.6 19,444 + GFF663 0.44 -0.4 19,478 + GFF663 0.47 -0.8 19,614 + GFF663 0.58 +0.3 19,713 - GFF663 0.66 +3.5 20,067 + +0.8 20,068 - +0.6 20,068 - +0.1 20,068 - -0.6 20,072 + -0.4 20,073 - -0.1 20,073 - +0.1 20,094 + -0.2 20,178 + +0.2 20,179 - -0.1 20,180 + -0.7 20,180 + -0.0 20,181 - -0.7 20,181 - +0.6 20,581 - GFF664 0.48 -0.1 20,842 - GFF664 0.76 -0.0 20,855 + GFF664 0.77 -1.0 20,855 + GFF664 0.77 -0.8 21,114 + +0.9 21,114 + +0.1 21,115 - -0.2
Or see this region's nucleotide sequence