Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF587 and GFF588 are separated by 198 nucleotides GFF588 and GFF589 are separated by 75 nucleotides GFF589 and GFF590 are separated by 93 nucleotides GFF590 and GFF591 overlap by 4 nucleotides
GFF587 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 322,840 to 324,348
GFF587
GFF588 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 324,547 to 324,873
GFF588
GFF589 - GlpG protein (membrane protein of glp regulon), at 324,949 to 325,779
GFF589
GFF590 - Glycerol-3-phosphate regulon repressor, DeoR family, at 325,873 to 326,631
GFF590
GFF591 - Transcriptional regulatory protein RtcR, at 326,628 to 328,211
GFF591
Position (kb) 324
325
326 Strain fitness (log2 ratio) -1
0
1
2
3 at 324.027 kb on - strand, within GFF587 at 324.191 kb on - strand, within GFF587 at 324.451 kb on + strand at 324.452 kb on - strand at 324.455 kb on + strand at 324.455 kb on + strand at 324.456 kb on - strand at 324.456 kb on - strand at 324.457 kb on + strand at 324.476 kb on + strand at 324.489 kb on + strand at 324.490 kb on - strand at 324.512 kb on - strand at 324.564 kb on + strand at 324.564 kb on + strand at 324.564 kb on + strand at 324.565 kb on - strand at 324.584 kb on - strand, within GFF588 at 324.735 kb on - strand, within GFF588 at 324.743 kb on - strand, within GFF588 at 324.756 kb on + strand, within GFF588 at 324.776 kb on - strand, within GFF588 at 324.793 kb on + strand, within GFF588 at 324.909 kb on - strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.912 kb on + strand at 324.912 kb on + strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.927 kb on + strand at 325.004 kb on - strand at 325.029 kb on + strand at 325.056 kb on + strand, within GFF589 at 325.270 kb on + strand, within GFF589 at 325.270 kb on + strand, within GFF589 at 325.271 kb on - strand, within GFF589 at 325.399 kb on - strand, within GFF589 at 325.427 kb on - strand, within GFF589 at 325.469 kb on + strand, within GFF589 at 325.508 kb on - strand, within GFF589 at 325.508 kb on - strand, within GFF589 at 325.512 kb on - strand, within GFF589 at 325.562 kb on - strand, within GFF589 at 325.692 kb on + strand, within GFF589 at 325.927 kb on + strand at 326.160 kb on + strand, within GFF590 at 326.350 kb on - strand, within GFF590 at 326.388 kb on + strand, within GFF590 at 326.400 kb on + strand, within GFF590 at 326.428 kb on + strand, within GFF590 at 326.522 kb on + strand, within GFF590 at 326.538 kb on + strand, within GFF590 at 326.539 kb on - strand, within GFF590 at 326.557 kb on + strand at 326.610 kb on + strand at 326.675 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 324,027 - GFF587 0.79 +0.6 324,191 - GFF587 0.90 -0.6 324,451 + -0.2 324,452 - -0.2 324,455 + -0.4 324,455 + -1.7 324,456 - -0.2 324,456 - +1.9 324,457 + +1.5 324,476 + -0.1 324,489 + -0.1 324,490 - +2.2 324,512 - -0.1 324,564 + +0.0 324,564 + +0.3 324,564 + +0.0 324,565 - -0.5 324,584 - GFF588 0.11 -0.3 324,735 - GFF588 0.57 -0.7 324,743 - GFF588 0.60 +0.4 324,756 + GFF588 0.64 -0.3 324,776 - GFF588 0.70 -0.2 324,793 + GFF588 0.75 +1.1 324,909 - -1.5 324,910 + +0.3 324,910 + +0.8 324,910 + +0.6 324,910 + +0.1 324,910 + +0.7 324,910 + +2.8 324,910 + -0.5 324,910 + +0.1 324,910 + +0.3 324,911 - +0.8 324,911 - +0.3 324,911 - -0.4 324,911 - +1.8 324,911 - -0.4 324,911 - -0.0 324,911 - +0.0 324,911 - +0.5 324,911 - -0.0 324,912 + +0.2 324,912 + -0.7 324,913 - +0.6 324,913 - -0.6 324,913 - -1.2 324,913 - +0.5 324,927 + +0.3 325,004 - +0.2 325,029 + -0.2 325,056 + GFF589 0.13 +0.8 325,270 + GFF589 0.39 +0.3 325,270 + GFF589 0.39 +1.3 325,271 - GFF589 0.39 +0.3 325,399 - GFF589 0.54 +1.8 325,427 - GFF589 0.58 -1.7 325,469 + GFF589 0.63 +1.9 325,508 - GFF589 0.67 -0.5 325,508 - GFF589 0.67 +0.1 325,512 - GFF589 0.68 +0.5 325,562 - GFF589 0.74 +1.3 325,692 + GFF589 0.89 -0.3 325,927 + -0.6 326,160 + GFF590 0.38 +0.4 326,350 - GFF590 0.63 +0.5 326,388 + GFF590 0.68 +0.4 326,400 + GFF590 0.69 +0.2 326,428 + GFF590 0.73 +0.2 326,522 + GFF590 0.86 +0.0 326,538 + GFF590 0.88 +0.4 326,539 - GFF590 0.88 +0.8 326,557 + +0.2 326,610 + -0.0 326,675 + +0.1
Or see this region's nucleotide sequence