Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF55 and GFF56 are separated by 29 nucleotides GFF56 and GFF57 are separated by 36 nucleotides GFF57 and GFF58 are separated by 246 nucleotides GFF58 and GFF59 are separated by 88 nucleotides
GFF55 - [Protein-PII] uridylyltransferase (EC 2.7.7.59), at 36,274 to 38,946
GFF55
GFF56 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 38,976 to 39,800
GFF56
GFF57 - Permeases of the major facilitator superfamily, at 39,837 to 41,138
GFF57
GFF58 - UPF0325 protein YaeH, at 41,385 to 41,771
GFF58
GFF59 - Sugar diacid utilization regulator SdaR, at 41,860 to 43,017
GFF59
Position (kb)
39
40
41
42 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 38.877 kb on + strand at 38.878 kb on - strand at 38.880 kb on + strand at 38.957 kb on + strand at 38.959 kb on + strand at 39.792 kb on - strand at 39.938 kb on + strand at 39.944 kb on - strand at 40.030 kb on + strand, within GFF57 at 40.035 kb on + strand, within GFF57 at 40.036 kb on - strand, within GFF57 at 40.106 kb on + strand, within GFF57 at 40.249 kb on - strand, within GFF57 at 40.249 kb on - strand, within GFF57 at 40.287 kb on + strand, within GFF57 at 40.472 kb on + strand, within GFF57 at 40.532 kb on + strand, within GFF57 at 40.532 kb on + strand, within GFF57 at 40.532 kb on + strand, within GFF57 at 40.534 kb on + strand, within GFF57 at 40.534 kb on + strand, within GFF57 at 40.534 kb on + strand, within GFF57 at 40.535 kb on - strand, within GFF57 at 40.535 kb on - strand, within GFF57 at 40.535 kb on - strand, within GFF57 at 40.536 kb on + strand, within GFF57 at 40.537 kb on - strand, within GFF57 at 40.537 kb on - strand, within GFF57 at 40.626 kb on + strand, within GFF57 at 40.629 kb on - strand, within GFF57 at 40.632 kb on + strand, within GFF57 at 40.714 kb on + strand, within GFF57 at 40.815 kb on - strand, within GFF57 at 40.875 kb on - strand, within GFF57 at 40.878 kb on + strand, within GFF57 at 40.904 kb on + strand, within GFF57 at 40.949 kb on + strand, within GFF57 at 40.950 kb on - strand, within GFF57 at 40.981 kb on - strand, within GFF57 at 41.053 kb on - strand at 41.085 kb on + strand at 41.118 kb on - strand at 41.124 kb on - strand at 41.126 kb on - strand at 41.139 kb on - strand at 41.234 kb on + strand at 41.238 kb on + strand at 41.239 kb on - strand at 41.239 kb on - strand at 41.496 kb on - strand, within GFF58 at 41.528 kb on + strand, within GFF58 at 41.529 kb on - strand, within GFF58 at 41.533 kb on - strand, within GFF58 at 41.581 kb on - strand, within GFF58 at 41.582 kb on + strand, within GFF58 at 41.582 kb on + strand, within GFF58 at 41.723 kb on + strand, within GFF58 at 41.766 kb on - strand at 41.819 kb on + strand at 41.821 kb on + strand at 41.821 kb on + strand at 41.822 kb on - strand at 41.823 kb on + strand at 41.841 kb on - strand at 41.892 kb on - strand at 41.892 kb on - strand at 41.994 kb on + strand, within GFF59 at 41.995 kb on - strand, within GFF59 at 42.049 kb on + strand, within GFF59 at 42.050 kb on - strand, within GFF59 at 42.050 kb on - strand, within GFF59 at 42.052 kb on + strand, within GFF59
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 38,877 + -2.3 38,878 - -1.2 38,880 + +1.9 38,957 + +0.2 38,959 + -0.2 39,792 - -2.1 39,938 + -0.1 39,944 - -0.5 40,030 + GFF57 0.15 -1.8 40,035 + GFF57 0.15 +0.5 40,036 - GFF57 0.15 +0.4 40,106 + GFF57 0.21 +0.8 40,249 - GFF57 0.32 +0.9 40,249 - GFF57 0.32 +0.7 40,287 + GFF57 0.35 +0.4 40,472 + GFF57 0.49 +0.5 40,532 + GFF57 0.53 -0.1 40,532 + GFF57 0.53 -0.3 40,532 + GFF57 0.53 -0.1 40,534 + GFF57 0.54 +0.2 40,534 + GFF57 0.54 +0.4 40,534 + GFF57 0.54 -0.2 40,535 - GFF57 0.54 +1.5 40,535 - GFF57 0.54 -0.2 40,535 - GFF57 0.54 +0.1 40,536 + GFF57 0.54 +4.3 40,537 - GFF57 0.54 -1.8 40,537 - GFF57 0.54 -0.1 40,626 + GFF57 0.61 -0.5 40,629 - GFF57 0.61 +0.2 40,632 + GFF57 0.61 +0.0 40,714 + GFF57 0.67 +0.8 40,815 - GFF57 0.75 +1.2 40,875 - GFF57 0.80 +1.2 40,878 + GFF57 0.80 +0.8 40,904 + GFF57 0.82 +0.3 40,949 + GFF57 0.85 +2.1 40,950 - GFF57 0.85 +0.3 40,981 - GFF57 0.88 +0.5 41,053 - -0.1 41,085 + +2.1 41,118 - -0.1 41,124 - +0.5 41,126 - +0.2 41,139 - +0.2 41,234 + -0.8 41,238 + +0.0 41,239 - -0.6 41,239 - -0.1 41,496 - GFF58 0.29 +1.2 41,528 + GFF58 0.37 -0.4 41,529 - GFF58 0.37 -0.3 41,533 - GFF58 0.38 +1.0 41,581 - GFF58 0.51 +0.1 41,582 + GFF58 0.51 -0.6 41,582 + GFF58 0.51 -0.4 41,723 + GFF58 0.87 -1.5 41,766 - -0.2 41,819 + -0.5 41,821 + -0.7 41,821 + +0.8 41,822 - -0.0 41,823 + -0.4 41,841 - +0.1 41,892 - +0.2 41,892 - -0.5 41,994 + GFF59 0.12 -2.1 41,995 - GFF59 0.12 -0.1 42,049 + GFF59 0.16 -0.1 42,050 - GFF59 0.16 +0.6 42,050 - GFF59 0.16 -0.7 42,052 + GFF59 0.17 +0.7
Or see this region's nucleotide sequence